Search results for "Fluorescent"

Influence of pea genotype on root associated fluorescent pseudomonads, impact on plant iron nutrition

2019

International audience; Pea has a high potential in agroecology because of its ability to fix atmospheric nitrogen and for Humannutrition due to the high amino-acids content of its seeds. However, pea can suffer from a susceptibility toiron deficiency in calcareous soils as expressed by chlorosis symptoms. Previous studies have shown thatsiderophores of model strains of fluorescent pseudomonads (fp), pyoverdines, promote iron nutrition ofarabidopsis and tobacco. We hypothesized that susceptibility to iron deficiency of pea is at least partly dueto its ability to select fluorescent pseudomonad that promote differentially plant nutrition thanks to theirsiderophores.To identify siderophores po…

Impact du cuivre sur les populations de Pseudomonas fluorescents. Variabilité génétique et capacités adaptatives

1999

National audience

Interactions réciproques entre plantes et Pseudomonas fluorescents en relation avec le fer rhizosphèrique

2009

Reciprocal interactions between plants and fluorescent pseudomonads in relation with iron in the rhizosphere

2007

International audience; Iron is an essential element for plants and microbes. However, in most cultivated soils, the concentration of iron available for these living organisms is very low since its solubility is controlled by stable hydroxides, oxyhydroxides and oxides. The high demand for iron by plants and microorganisms in the rhizosphere together with its low availability in soils leads to a strong competition for this nutrient among living organisms. To face this competition, plants and microorganisms have developed active strategies of iron uptake. In non graminaceous plants (strategy I), iron uptake relies on acidification and reduction of Fe+++ in Fe++ which incorporated in the root…

Interactions between Pisum sativum & pseudomonads, consequences on plant iron nutrition and growth and immunity

2019

Pea has a high potential in agroecology, however, it may suffer from a susceptibility to iron deficiency in calcareous soils. This susceptibility varies depending on cultivars. Pseudomonad siderophores, pyoverdines (pvd), were shown to promote iron nutrition in Arabidopsis and tobacco. The hypothesis was therefore raised that variation in iron susceptibility between pea cultivars could be related to differences in their ability to recruit fluorescent pseudomonads (fp) contributing to siderophore-mediated iron nutrition. Our aims were to compare i) the impact of a susceptible (S) and a tolerant (T) cultivar of pea on fp biodiversity with a focus on their pvd, and in return ii) the impact of …

Extraction of copper and iron from soil with siderophores of fluorescent pseudomonads

2009

Due to the application for more than 100 years of copper to French vineyards to combat fungal diseases of vines copper levels have been reported in vineyard soils up to several hundred milligrams of copper/kg of soil. There are now concerns about the ecotoxicity effects of copper accumulation. One potential way to remediate copper accumulation is phytoextraction. Furthermore, there may be potential to enhance the phytoextraction process by augmenting the process using beneficial bacterial species. Our research has involved investigating the potential to use fluorescent pseudomonads, a key group of bacteria that produce siderophores (iron-scavenging compounds), to enhance phytoextraction of …

Influence du système de sécrétion de type III bactérien dans les intéractions plantes-Pseudomonas spp. fluorescents non pathogènes

2012

http://prodinra.inra.fr/record/271693SPEEAEcolDurCT3; L’objectif de cette thèse a été de contribuer à faire progresser les connaissances sur les interactions bénéfiques entre les plantes et les microorganismes en évaluant la contribution des systèmes de sécrétion de type III (SST3). Une synthèse des connaissances disponibles relatives aux SST3 chez les Pseudomonas non pathogènes, saprotrophes ou mutualistes, montre que les SST3 ne sont pas cantonnés aux interactions parasites ou pathogènes avec les plantes. Dans la première étude expérimentale, nous avons utilisé différents génotypes de Medicago truncatula Gaertn. cv. Jemalong capables (Myc+) ou non (Myc-) d’établir une symbiose mycorhizien…

Approche tridimensionnelle de la répartition spatiale du champignon mycorhizien à arbuscule sur racines transparisées

2022

L’imagerie tridimensionnelle d’échantillons volumineux est limitée par les phénomènes de réfraction et d’absorption de la lumière par les composants tissulaires (lipides, pigments...). Afin de limiter ses phénomènes et permettre l’acquisition d’image sur toute l’épaisseur d’une racine mycorhizée, il est nécessaire de rendre transparent les échantillons. Cela est possible en dépigmentant et en homogénéisant les différents indices de réfraction au sein du tissu, via des traitements chimiques. Cette technique, appelée transparisation, permet une analyse tridimensionnelle sur échantillon entier des structures d’intérêt marquées par fluorescence. Une vingtaine de techniques différentes de clarif…

Coumarin-Phosphine-Based Smart Probes for Tracking Biologically Relevant Metal Complexes: From Theoretical to Biological Investigations

2016

International audience; Ten metal-based complexes and associated ligands have been synthesized and characterized. One of the metal ligands is a coumarin-phosphine derivative, which displays tunable fluorescence properties. The fluorescence is quenched in the case of the free ligand and ruthenium and osmium complexes, whereas it is strong for the gold complexes and phosphonium derivatives. These trends were rationalized by theoretical calculations, which revealed non-radiative channels involving a dark state for the free ligands that is lower in energy than the emissive state and is responsible for the quenching of fluorescence. For the Ru-II and Os-II complexes, other non-radiative channels…

A novel homologous model for noninvasive monitoring of endometriosis progression.

2017

To date, several groups have generated homologous models of endometriosis through the implantation of endometrial tissue fluorescently labeled by green fluorescent protein (GFP) or tissue from luciferase-expressing transgenic mice into recipient animals, enabling noninvasive monitoring of lesion signal. These models present an advantage over endpoint models, but some limitations persist; use of transgenic mice is laborious and expensive, and GFP presents poor tissue penetration due to the relatively short emission wavelength. For this reason, a homologous mouse model of endometriosis that allows in vivo monitoring of generated lesions over time and mimics human lesions in recipient mice wou…