Search results for "Forensic"

Clinicopathologic and DNA Flow Cytometric Analysis of Eighty-three Renal Cell Carcinomas

1995

The clinical and anatomopathologic features as well as DNA content of 83 renal cell carcinomas were analyzed. The possible interrelationship and prognostic value of these factors were also considered. The DNA study has been performed by flow cytometry using paraffin-embedded tissues. In each case an internal control with nonneoplastic renal tissue obtained in the same nefrectomy was also done in order to calculate the DNA index. Differences in patient age, nuclear grade, and mitotic activity were related with outcome; however, this relationship was not confirmed by the Cox test. Stage (P = .005), cell type (P = .002), and metastatic disease (P = .001) had independent prognostic values. Ren…

Notch signalling is off and is uncoupled from HES1 expression in Ewing's sarcoma

2010

Notch can act as an oncogene or as a tumour suppressor and thus can either promote or inhibit tumour cell growth. To establish Notch status in Ewing's sarcoma family of tumours (ESFT), we investigated the Notch pathway by gene expression profiling meta-analysis or immunohistochemistry in samples obtained from 96 and 24 ESFT patients, respectively. We found that although Notch receptors were highly expressed, Notch did not appear to be active, as evidenced by the absence of Notch receptors in cell nuclei. In contrast, we show that Notch receptors known to be active in colon adenocarcinoma, hepatocarcinoma, and pancreatic carcinoma stain cell nuclei in these tumours. High expression of the No…

Adipose tissue sensitivity to radiation exposure

2008

1525-2191 (Electronic) Journal Article Research Support, Non-U.S. Gov't; Treatment of cancer using radiation can be significantly compromised by the development of severe acute and late damage to normal tissue. Treatments that either reduce the risk and severity of damage or that facilitate the healing of radiation injuries are being developed, including autologous adipose tissue grafts to repair tissue defects or involutional disorders that result from tumor resection. Adipose tissue is specialized in energy storage and contains different cell types, including preadipocytes, which could be used for autologous transplantation. It has long been considered a poorly proliferative connective ti…

Unusual Intranuclear Inclusions in Malignant Fibrous Histiocytoma: Presence in Primary Tumor

1982

We describe previously unreported intranuclear inclusions in 2 cases of malignant fibrous histiocytoma. The inclusions were found in 2-10% of the tumor cells removed from the patients and in 2-10% of the cells examined in tumor tissue xenotransplanted in nude mice. By stereo electron microscopy the inclusions are closely packed undulating fibrils 18-23 nm in diameter. They are sometimes associated with fibrillary bodies. They closely resemble the inclusions reported in some animals inoculated with serum from patients with non-A non-B hepatitis; however, their nature at present is unknown.

Bloodstains on Leather: Examination of False Negatives in Presumptive Test and Human Hemoglobin Test.

2017

Presumptive tests for blood are very simple and sensitive tests used in the search for evidence. They also provide initial information on the nature of stains. A second test can confirm their nature. However, these tests can present false–negative results for different reasons. Some of those reasons have been studied, while others, those caused by the substrate material that contains the stain, are less well known. This work studies the effect of one component of a leather substrate—quebracho extract—on presumptive and human hemoglobin blood tests. Assays were performed using samples of blood dilutions contaminated with quebracho extract and others formed on a substrate containing the conta…

Investigation of bloodstains: false negative results of the benzidine test

1995

Since the discovery of the Benzidine Test, this method has been considered absolutely reliable for the generic diagnosis of bloodstains. The authors reviewed support the existence of false positive results, as the subtract in the coloration reaction the peroxidases may be found in numerous natural products [ 11. There is also general agreement with the false negative results: a negative result provided by the Benzidine Test indicates that the stain is not blood [2]. Given the strong certainty offered by most works of forensic medicine and criminology, most countries Spain included use this technique as a screening method in the investigation of bloodstains: when the result is negative the i…

Genomic profiling to assess the clonal relationship between histologically distinct intracranial tumours

2012

Primary glioblastomas with and without EGFR amplification: Relationship to genetic alterations and clinicopathological features

2009

Glioblastomas express a notable heterogeneity in both the histological and cell patterns with glial astrocytic differentiation. Primary glioblastoma, which is the most frequent presentation (90-95%), occurs mainly in older patients and arises de novo, without any clinical or histological evidence of a less malignant precursor lesion. EGFR amplification has been identified as a genetic hallmark of primary glioblastomas and occurs in 40-60% of cases. However, there exist primary glioblastomas without EGFR amplification/overexpression. The purpose of this study was to stabilize the association between cases with and without EGFR gene amplification with clinical and genetic parameters in 45 cas…

Experimental emetine myopathy: enzyme histochemical, electron microscopic, and immunomorphological studies.

1993

Ipecac, containing emetine hydrochloride, is used by patients with anorexia nervosa to induce vomiting. Its chronic usage may result in a myopathy and a cardiomyopathy, the former marked by cytoplasmic bodies. We studied myopathological changes after daily injections of female Wistar rats with emetine hydrochloride intraperitoneally for periods of 4, 5, 9, and 10 weeks. the extensor digitorum longus muscle and the soleus muscle showed core-like lesions, streaming of the z-discs, nemaline bodies, cytoplasmic bodies, and spheroid cytoplasmic bodies. Immunomorphological studies revealed increased amounts of desmin. During a period of repair, i.e., 2, 4, and 6 weeks after termination of emetine…

Endothelial capillaries chemotactically attract tumour cells.

2001

Directional migration of capillaries towards tumour implants is generally assumed to be regulated by chemotaxis. Preliminary evidence has also been presented for the existence of a reverse chemotactic signalling pathway, with capillaries attracting tumour cells via paracrine factors. By using a variety of endothelial cell types and tumour cell lines, this study has systematically investigated chemotaxis between endothelial cells and tumour cells in two- and three-dimensional systems. Checkerboard analysis revealed faint attraction of human umbilical vein endothelial cells (HUVECs), but not porcine aortic endothelial cells (PAECs), by tumour cells. In reverse, both PAECs and HUVECs potently …