Search results for "Forensic"

showing 10 items of 1701 documents

Fluorescence Microscopy as a Diagnostic Tool for Dermatophytosis.

2016

Dermatophytosis is a superficial fungal infection of keratinized tissues. Dermatophytes can cause discomfort but are not usually life threatening. However, the infection can spread and may lead to systemic fungal infections in immunocompromised patients. Currently available diagnostic methods include potassium hydroxide (KOH) testing and periodic acid-Schiff (PAS) staining. However, most diagnostic techniques cannot be performed rapidly; days to weeks may be required for conclusive results. Certain dermatophytes autofluoresce and can be observed under fluorescence microscopy. The authors examined a series of 24 cases of hematoxylin and eosin-stained dermatophytoses using fluorescence micros…

AdultMalePathologymedicine.medical_specialtyDiagnostic methodsAdolescentH&E stainDermatologyPeriodic acid–Schiff stainPathology and Forensic Medicine030207 dermatology & venereal diseases03 medical and health sciencesYoung Adult0302 clinical medicineTineaMicroscopyFluorescence microscopeMedicineHumans030212 general & internal medicineChildAgedAged 80 and overbusiness.industryGeneral MedicineMiddle AgedFluorescenceStainingClinical PracticeMicroscopy FluorescenceFemalebusinessThe American Journal of dermatopathology
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The Glomerular Lesions in Endotheliotropic Hemolytic Nephroangiopathy (Hemolytic Uremic Syndrome, Malignant Nephrosclerosis, Post Partal Renal Insuff…

1982

The term "Endotheliotropic Hemolytic Nephroangiopathy" (EHN) comprises various clinically or pathomorphologically defined disease states with severe renal lesions (e.g. hemolytic uremic syndrome, malignant nephrosclerosis, post partum renal insufficiency) which, to date, have been considered as different entities. We attempted to assign accompanying glomerular changes based upon light and electron microscopy to the above mentioned clinical pictures and their various stages. The accordingly classified glomerular lesions (G1--G3 and Ga) are of critical importance in pathohistological differential diagnosis. Since it is assumed that fibrin is a causing event in the pathogenesis of the vascular…

AdultMalePathologymedicine.medical_specialtyEndotheliumBiopsyKidney GlomerulusDiseaseFibrinPathology and Forensic MedicinePathogenesisPregnancyGlomerulopathyBiopsymedicineHumansChildAgedFibrinNephrosclerosisbiologymedicine.diagnostic_testMalignant nephrosclerosisbusiness.industryInfantPuerperal DisordersCell BiologyAcute Kidney InjuryMiddle Agedmedicine.diseaseMicroscopy Electronmedicine.anatomical_structureChild PreschoolHemolytic-Uremic Syndromebiology.proteinFemaleDifferential diagnosisbusinessPathology - Research and Practice
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Microvascular injury and repair in acute human bacterial pyelonephritis.

1987

Acute inflammatory cell-capillary endothelial cell interactions, related to injury and repair, were investigated light and electron microscopically in acute human bacterial pyelonephritis. In inflammatory infiltrate-adjacent microvessels, the small capillaries were completely occluded by leukocyte plugs and the large capillaries were densely filled with acute inflammatory cells adhering to the endothelium. Severe damage to small and large capillaries was observed around endothelium adherent, degranulated neutrophil granulocytes containing phagocytosed bacteria. There were spaces in the endothelium, degradation of the vascular basement membrane, of the perivascular interstitial matrix and of…

AdultMalePathologymedicine.medical_specialtyEndotheliumNeutrophilsKidneyFibrinPathology and Forensic MedicineNeovascularizationInterstitial spaceInterstitial matrixmedicineHumansMolecular BiologyAgedBasement membranebiologyNeovascularization PathologicPyelonephritisbusiness.industryCell BiologyGeneral MedicineBacterial InfectionsMiddle AgedCapillariesEndothelial stem cellMicroscopy Electronmedicine.anatomical_structureImmunologyAcute Diseasebiology.proteinFemalemedicine.symptombusinessBlood vesselVirchows Archiv. A, Pathological anatomy and histopathology
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Hepatitis A-like non-A, non-B hepatitis: light and electron microscopic observations of three cases.

1986

To date, three types of NANBH have been distinguished by epidemiological, clinical and experimental data. We examined the liver biopsies of three patients with an acute NANBH resembling hepatitis A from the infection route, incubation period and clinical course. The liver biopsies revealed lesions with a portal and periportal predominance, thus also exhibiting parallels with hepatitis A on the histopathological level.

AdultMalePathologymedicine.medical_specialtyHepatitis Viral HumanBiologyPathology and Forensic MedicineIncubation periodHLA AntigensPregnancymedicineHumansMolecular BiologyElectron microscopicClinical courseHistocompatibility Antigens Class IIHepatitis ACell BiologyGeneral MedicineHLA-DR AntigensHepatitis AMiddle Agedmedicine.diseaseMicroscopy ElectronImmunologic TechniquesNon b hepatitisFemaleViral diseaseVirchows Archiv. A, Pathological anatomy and histopathology
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Expression of Peripheral Node Addressins by Plasmacytic Plaque of Children, APACHE, TRAPP, and Primary Cutaneous Angioplasmacellular Hyperplasia.

2018

High-endothelial venules are a common feature of 3 types of cutaneous pseudolymphomas: pretibial lymphoplasmacytic plaque (PLP) of children, acral pseudolymphomatous angiokeratoma of children (APACHE), and T-cell rich angiomatoid polypoid pseudolymphoma (TRAPP). In addition, primary cutaneous angioplasmacellular hyperplasia (PCAH) overlaps with these other 3 conditions. We intend to study the expression of peripheral node addressins in PLP, APACHE, TRAPP, and PCAH. We studied 1 case of PLP, 2 cases of APACHE, 2 cases of TRAPP, and 2 cases of PCAH. Immunostainings for MECA-79 and WT-1 were obtained in all cases. All cases showed a dense lymphohistiocytic dermal inflammatory infiltrate with a…

AdultMalePathologymedicine.medical_specialtyHistologyAdolescentPlasma CellsImmunoglobulinsPathology and Forensic MedicineDiagnosis Differential030207 dermatology & venereal diseases03 medical and health sciencesYoung Adult0302 clinical medicineImmunophenotypingMucoproteinsPseudolymphomaAddressinmedicinePseudolymphomaHumansSkin pathologyChildAgedSkinAged 80 and overHyperplasiabiologybusiness.industryHyperplasiaMiddle Agedmedicine.diseaseImmunohistochemistryPeripheralAngiokeratomaMedical Laboratory Technology030220 oncology & carcinogenesisChild Preschoolbiology.proteinImmunohistochemistryFemaleLymph NodesbusinessCell Adhesion MoleculesAngiokeratomaApplied immunohistochemistrymolecular morphology : AIMM
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Local reactions to tick bites

2008

A retrospective histological and immunohistochemical study has been carried out in 25 cases of tick bites recorded in our Departments. The samples that included an attached tick showed a cement cone anchoring the mouthparts to the skin and a blood-soaked, spongiform appearance of the superficial dermis, with a mild neutrophilic and eosinophilic infiltration. The vessels displayed a loose multilayered endothelial proliferation, with plump endothelia, permeated with erythrocytes. A few of them were severed, allowing copious blood extravasation. The established lesions included the following: erythema chronicum migrans-like cases, foreign body granulomas-sometimes containing remnants of the mo…

AdultMalePathologymedicine.medical_specialtyHistologyErythemaAdolescentAlopecia AreataT-LymphocytesDermatologyBiologySettore MED/08 - Anatomia PatologicaSkin DiseasesLymphoid hyperplasiaPathology and Forensic MedicineHost-Parasite InteractionsLymphocytic InfiltrateDermisPseudolymphomamedicineSettore MED/35 - Malattie Cutanee E VenereeAnimalsHumansChildAgedRetrospective StudiesAged 80 and overB-LymphocytesIxodeslocal reactionInsect Bites and StingsHistologyGeneral MedicineAnatomyHyperplasiaMiddle Agedmedicine.diseaseHistology; local reactions; tick attacksArthropod mouthpartsExtravasationmedicine.anatomical_structuretick attacksErythema Chronicum MigransFemalemedicine.symptom
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Intrasinusoidal bone marrow infiltration: a possible hallmark of splenic lymphoma.

1996

A particular type of lymphomatous bone marrow infiltration defined as intrasinusoidal and occuring in seven patients with splenomegaly and mild to moderate thrombocytopenia is reported. Immunocytochemistry highlighted the intrasinusoidal pattern and showed a mature B-cell phenotype. Four patients underwent splenectomy and a diagnosis of splenic marginal cell lymphoma was made. The disease course seems to be chronic and quite indolent. All patients are alive and well ; follow-up ranges from 2 to 42 months. The value of bone marrow biopsy as a primary diagnostic tool in splenic marginal zone lymphomas is discussed.

AdultMalePathologymedicine.medical_specialtyHistologyLymphoma B-Cellmedicine.medical_treatmentSplenectomySpleenPathology and Forensic MedicineImmunophenotypingSinusoidBone MarrowBiopsymedicineHumansAgedmedicine.diagnostic_testbusiness.industryLymphoma Non-HodgkinSplenic NeoplasmsBone Marrow ExaminationGeneral MedicineMiddle Agedmedicine.diseaseImmunohistochemistrymedicine.anatomical_structureImmunohistochemistryFemaleBone marrowSplenic diseaseSplenic LymphomabusinessHistopathology
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Dermatofibrosarcoma protuberans: clinical, pathological, and genetic (COL1A1-PDGFB ) study with therapeutic implications.

2009

Aims:  To analyse the presence of collagen type I alpha 1–platelet-derived growth factor beta (COL1A1–PDGFB) transcripts in 20 cases of dermatofibrosarcoma protuberans (DFSP) and to assess the relationship between COL1A1 breakpoints and clinical and histopathological variables. Methods and results:  Multiplex reverse transcriptase-polymerase chain reaction was carried out using frozen tissue. Our series contained 14 men and six women. Histologically, most cases were of conventional type (n = 9), followed by fibrosarcoma (n = 4), Bednar tumour (n = 2), sclerosing (n = 2), myoid (n = 1) and atrophic (n = 1) DFSP, and giant cell fibroblastoma (n = 1). Immunohistochemistry revealed CD34 express…

AdultMalePathologymedicine.medical_specialtyHistologySkin NeoplasmsAdolescentCD34Antineoplastic AgentsBiologyCollagen Type IPiperazinesPathology and Forensic MedicineYoung AdultDermatofibrosarcoma protuberansmedicineHumansAgedDNA PrimersAged 80 and overPDGFBBase SequenceDermatofibrosarcomaGeneral MedicineGiant-cell fibroblastomaMiddle Agedmedicine.diseaseMohs SurgeryCollagen Type I alpha 1 ChainImatinib mesylatePyrimidinesFusion transcriptCOL1A1/PDGFB Fusion GeneBenzamidesImatinib MesylateFemaleGene FusionDermatofibrosarcomaGenes sisHistopathology
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Testicular fusocellular rhabdomyosarcoma as a metastasis of elbow sclerosing rhabdomyosarcoma: A clinicopathologic, immunohistochemical and molecular…

2010

Abstract Sclerosing rhabdomyosarcoma (SRMS) is an infrequent variant of rhabdomyosarcoma characterized by extensive intercellular hyaline fibrosis. We report the case of a 37 year-old male with a 9 × 6 cm SRMS on the right elbow. Histologically, the tumor showed an abundant extracellular hyaline matrix with extratumoral vascular emboli and microscopic foci of fusocellular embryonal rhabdomyosarcoma (FRMS) separated by a fibrotic band from the sclerosing areas. One year later the patient presented with a right intratesticular tumor of 1.2 × 0.8 cm, which was reported as pure FRMS. Immunohistochemically, SRMS was positive only for MyoD1 and Vimentin and negative for Myogenin and Desmin. Both …

AdultMalePathologymedicine.medical_specialtyHistologyTime FactorsVimentinCase ReportSoft Tissue NeoplasmsSclerosing rhabdomyosarcomaBiologyTranslocation GeneticPathology and Forensic MedicineMetastasisFatal OutcomeTesticular Neoplasmslcsh:PathologymedicineBiomarkers TumorElbowHumansRhabdomyosarcoma EmbryonalWhole Body ImagingRhabdomyosarcomaHyalineIn Situ Hybridization FluorescenceSclerosisChromosomes Human Pair 13Forkhead Box Protein O1MediastinumForkhead Transcription FactorsGeneral Medicinemedicine.diseasemusculoskeletal systemFibrosisImmunohistochemistrymedicine.anatomical_structureTreatment OutcomeChemotherapy Adjuvantbiology.proteinDesminEmbryonal rhabdomyosarcomaTomography X-Ray ComputedOrchiectomylcsh:RB1-214
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CD27 distinguishes two phases in bone marrow infiltration of splenic marginal zone lymphoma.

2004

Aims:  To investigate CD27 expression in splenic marginal zone lymphoma (SMZL), an indolent low-grade B-cell lymphoma with constant involvement of the bone marrow, especially with an intrasinusoidal pattern. It is not clear if the neoplastic clone is composed of virgin or somatically mutated B cells. CD27 is reported to be a hallmark of memory B cells. Methods and results:  We evaluated 64 bone marrow biopsy specimens (BMBs) from 36 patients with SMZL for the expression of CD27. For comparison, splenectomy specimens of patients with traumatic splenic rupture or with SMZL were used. All BMBs showed lymphomatous infiltration. When located in the marrow sinusoids, neoplastic cells were CD27– i…

AdultMalePathologymedicine.medical_specialtyHistologybone marrowLymphomaBiopsyNaive B cellsplenic marginal zone lymphomaSplenic NeoplasmSpleenPathology and Forensic MedicineBiopsyintrasinusoidal infiltrationmedicineHumansSplenic marginal zone lymphomaCD27naive and memory B cellsAgedAged 80 and overmedicine.diagnostic_testbusiness.industrySplenic NeoplasmsGeneral MedicineMiddle Agedmedicine.diseaseBone Marrow NeoplasmImmunohistochemistryTumor Necrosis Factor Receptor Superfamily Member 7bone marrow; CD27; intrasinusoidal infiltration; naive and memory B cells; splenic marginal zone lymphomamedicine.anatomical_structureBone marrow neoplasmFemaleBone marrowbusinessBone Marrow NeoplasmsInfiltration (medical)
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