Search results for "Forming"
showing 10 items of 1719 documents
Laser speckle imaging for early detection of microbial colony forming units
2021
In this study, an optical contactless laser speckle imaging technique for the early identification of bacterial colony-forming units was tested. The aim of this work is to compare the laser speckle imaging method for the early assessment of microbial activity with standard visual inspection under white light illumination. In presented research, the growth of Vibrio natriegens bacterial colonies on the solid medium was observed and analyzed. Both – visual examination under white light illumination and laser speckle correlation analysis were performed. Based on various experiments and comparisons with the theoretical Gompertz model, colony radius growth curves were obtained. It was shown that…
Laser speckle time-series correlation analysis for bacteria activity detection
2020
The study aims at development and laboratory approbation of non-contact optical technique for early evaluation of microbial activity. Microorganisms’ activity is estimated by laser speckle contrast imaging technique in combination with image processing of obtained time varying speckle patterns. Laser speckle patterns were captured by CMOS sensor during illumination of growing bacteria colonies by low power (<30 mW, 635 nm) stabilized coherent light source. To validate proposed technique and image processing algorithm the vibrio natriegens bacteria are used. After analysis of several different experiments the following results were obtained: In the central part of the colony activity can be …
Behavior of four main dairy pathogenic bacteria during manufacturing and ripening of pecorino siciliano cheese
2020
Background: Consumption of raw cheese may be associated with different diseases. This study aimed to evaluate behavior of four pathogenic bacteria during manufacture and ripening of Protected Designation of Origin (PDO) Pecorino Siciliano cheese.
 Methods: The experimental cheese groups were inoculated with pathogenic bacteria, including Escherichia coli O157, Listeria monocytogenes, Salmonella Enteritidis, and Staphylococcus aureus. The cheese making processes were monitored from milk curdling until 3 months ripened cheeses and the levels of Lactic Acid Bacteria (LAB) and the four dairy pathogens were evaluated by plate counts. Randomly Amplified Polymorphic DNA (RAPD)-Polymerase Chai…
Complex Electromagnetic Fields Reduce Candida albicans Planktonic Growth and Its Adhesion to Titanium Surfaces
2021
This study evaluates the effects of different programs of complex electromagnetic fields (C.M.F.s) on Candida albicans, in planktonic and sessile phase and on human gingival fibroblasts (HGF cells). In vitro cultures of C. albicans ATCC 10231 and HGF cells were exposed to different cycles of C.M.F.s defined as: oxidative stress, oxidative stress/antibacterial, antibacterial, antibacterial/oxidative stress. Colony forming units (CFUs), metabolic activity, cells viability (live/dead), cell morphology, filamentation analysis, and cytotoxicity assay were performed. The broth cultures, exposed to the different C.M.F.s, were grown on titanium discs for 48 h. The quantity comparisons of adhered C.…
A variant of Smurf2 protects mice against colitis-associated colon cancer by inducing transforming growth factor β signaling.
2011
Background & Aims Transforming growth factor (TGF)-β signaling, which is down-regulated by the E3 ubiquitin ligase Smad ubiquitin regulating factor 2 (Smurf2), promotes development of cancer. We identified a splice variant of Smurf2 (ΔE2Smurf2) and investigated its role in colon carcinogenesis in mice. Methods Colitis-associated colon cancer was induced in mice by administration of azoxymethane, followed by 3 cycles of oral administration of dextran sodium sulfate. Messenger RNA levels of Smurf2 in colon tumors and control tissue were measured by quantitative polymerase chain reaction; lymphocyte and cytokine levels were measured in tumor and tissue samples. Results Tumor-infiltrating CD4 +…
TGF-β Dependent T-Cell Regulation in Colitis and Colon Cancer
2008
Transforming growth factor-β (TGF-β) is a potent growth inhibitor endowed with tumor-suppressing activity. Unfortunately, cancers are often resistant to such growth inhibition. This evasion frequently results from a genetic loss of functional TGF-β signaling components. On the other hand, cancer cells often produce high amounts TGF-β1 by themselves and sometimes respond to it with invasion and metastasis. Much effort is being done to develop therapeutic approaches to modulate TGF-β signaling in cancer cells either to inhibit the TGF-β-induced invasive phenotype or to reestablish its growth-inhibitory activities. However, TGF-β is a pleiotropic cytokine with important functions not only in c…
The Relative Importance of Local and Global Structures in Music Perception
2004
Research in experimental psychology has emotion in music is developed by L. B. Meyer.2 shown two paradoxes in music perception. By According to Meyer, listeners are not passive, mere exposure to musical pieces, Western lis- but rather constantly develop perceptual expectteners acquire sensitivity to the regularities ancies about the possible evolution of the underlying tonal music. This implicitly acquired music. Emotions arise from the way the comknowledge allows listeners to perceive subtle poser (or the improvising performer) fulfills relations between musical events and permits or frustrates these expectancies. To some musically untrained listeners to behave as music- extent, music perc…
Exploring relationships between pianists’ body movements, their expressive intentions, and structural elements of the music
2011
Body movements during music performance have been found to be indicative of the performer’s musical intentionality, and contribute to an observer’s perception of expressive playing. This study investigates the effect of structural elements of the score, and the playing of different levels of expression on body movements during a piano performance. Pianists were required to play the same piece in four different performance conditions. Their movements were tracked by an optical motion capture system, and the comparisons that were made between specific parts of the body used, performance condition, and musical score locations were subsequently statistically examined. We found that the head an…
Support vector machines in engineering: an overview
2014
This paper provides an overview of the support vector machine SVM methodology and its applicability to real-world engineering problems. Specifically, the aim of this study is to review the current state of the SVM technique, and to show some of its latest successful results in real-world problems present in different engineering fields. The paper starts by reviewing the main basic concepts of SVMs and kernel methods. Kernel theory, SVMs, support vector regression SVR, and SVM in signal processing and hybridization of SVMs with meta-heuristics are fully described in the first part of this paper. The adoption of SVMs in engineering is nowadays a fact. As we illustrate in this paper, SVMs can …
Recent Developments in the Design and Control of Cold Forming Processes
1999
In the paper some problems of process design and control in metal forming are taken into account. In particular as far as the former aspect is concerned, the problem of the preform design in cold forging is analyzed: the contributions offered by several researchers are presented and an innovative design procedure is proposed. As well, the suitability of closed-loop control systems for metal forming applications is discussed with particular reference to sheet stamping operations; again, after an overview of the state of the art, the advantages offered by the application in this field of Artificial Intelligence techiniques is pointed out.