Search results for "Frame"

Complete sequence, expression and evolution of two members of the hexamerin protein family during the larval development of the rice moth, Corcyra ce…

2002

Three distinct types of storage hexamerins are expressed in the "last-instar" larvae of the rice moth, Corcyra cephalonica. A cDNA expression library was constructed from fat body-RNA and screened with a polyclonal antibody raised against purified hexamerin (SP2) of Corcyra cephalonica. Two slightly different "full-length" hexamerin cDNA clones (Hex2a and Hex2b) were isolated and sequenced. Both include open reading frames of 2109 bp which are translated into polypeptides of 703 amino acids with 92.5% identity. Signal peptides of 19 amino acids are present at the N-termini. The 684 amino acids native proteins have a high content of aryl groups (17.6%). According to both the criteria for ami…

A novel cell wall protein specific to the mycelial form of Yarrowia lipolytica.

1996

A cDNA clone specifying a cell wall protein was isolated from a Yarrowia lipolytica cDNA library. The cDNA library was constructed in the expression vector lambda gt 11, with the RNA isolated from actively growing mycelial cells. The deduced amino acid sequence shows that the encoded protein contains an N-terminal hydrophobic signal peptide. We have designated this protein YWP1 for Yarrowia lipolytica cell Wall Protein. Northern hybridization identified YWP1 transcript only when Y. lipolytica was growing in the mycelial form. The encoded protein seems to be covalently bound to the glucan cell wall since it is not released from the cell walls by sodium dodecyl sulphate extraction, but it is …

A human multisystem disorder with autoinflammation, leukoencephalopathy and hepatopathy is caused by mutations in C2orf69

2021

AbstractBackgroundDeciphering the function of the many genes previously classified as uncharacterized “open reading frame” (orf) completes our understanding of cell function and its pathophysiology.MethodsWhole-exome sequencing, yeast 2-hybrid and transcriptome analyses together with molecular characterization are used here to uncover the function of the C2orf69 gene.ResultsWe identify loss-of-function mutations in the uncharacterized C2orf69 gene in eight individuals with brain abnormalities involving hypomyelination and microcephaly, liver dysfunction and recurrent autoinflammation. C2orf69 contains an N-terminal signal peptide that is required and sufficient for mitochondrial localizatio…

F17-like fimbriae from an invasive Escherichia coli strain producing cytotoxic necrotizing factor type 2 toxin

1994

The F17b fimbriae encoded by the transmissible virulence plasmid Vir, also coding for cytotoxic necrotizing factor type 2, were characterized. A 5.7-kb region of Vir mediates in vitro N-acetylglucosamine-sensitive adhesion to calf intestinal villi. Sequence analysis revealed that this region codes for a structural subunit and an adhesin closely related to the F17-A and F17-G proteins encoded by the F17 fimbrial gene cluster. The F17b-A gene presents an open reading frame of 540 bp encoding a polypeptide of 180 amino acids with a putative signal peptide of 21 residues. The mature protein shows an identity of 74% with the F17-A structural subunit. This 20-kDa protein is recognized by antiseru…

Views selection for SIFT based object modeling and recognition

2016

In this paper we focus on automatically learning object models in the framework of keypoint based object recognition. The proposed method uses a collection of views of the objects to build the model. For each object the collection is composed of N×M views obtained rotating the object around its vertical and horizontal axis. As keypoint based object recognition using a complete set of views is computationally expensive, we focused on the definition of a selection method that creates, for each object, a subset of the initial views that visually summarize the characteristics of the object and should be suited for recognition. We select the views by determining maxima and minima of a function, …

Flow and Deformation

1998

A hunter who investigates tracks in muddy ground near a waterhole may be able to reconstruct which animals arrived last, but older tracks will be partly erased or modified. A geologist faces similar problems to reconstruct the changes in shape that a volume of rock underwent in the course of geological time, since the end products, the rocks that are visible in outcrop, are the only direct data source. In many cases it is nevertheless possible to reconstruct at least part of the tectonic history of a rock from this final fabric. This chapter treats the change in shape of rocks and the methods that can be used to investigate and describe this change in shape. This is the field of kinematics,…

La legge sul sindacato militare: se vogliamo che tutto rimanga come è, bisogna che tutto cambi

2023

Il saggio analizza la recente legge sul sindacato militare a seguito della sentenza della Corte costituzionale 120/2018, che ha dichiarato l’incostituzionalità del divieto di costituire associazioni sindacali nelle Forze armate e di polizia a ordinamento militare. Alla luce dell’ordinamento multilivello (costituzione, convenzioni Oil, Carta Sociale e Cedu) l’articolo sottolinea l’irragionevole compressione legislativa della libertà sindacale a favore della coesione interna dell’apparato militare. Il filo rosso dei vari divieti e restrizioni risiede nella persistente adesione della politica agli obsoleti modelli teorici degli ordinamenti interni e dei rapporti di supremazia speciale, che non…

Inside Cover: A SIM-MOF: Three-Dimensional Organisation of Single-Ion Magnets with Anion-Exchange Capabilities (Chem. Eur. J. 34/2014)

2014

Massive presence of insertion sequences in the genome of SOPE, the primary endosymbiont of the rice weevil Sitophilus oryzae

2008

Bacteria that establish an obligate intracellular relationship with eukaryotic hosts undergo an evolutionary genomic reductive process. Recent studies have shown an increase in the number of mobile elements in the first stage of the adaptive process towards intracellular life, although these elements are absent in ancient endosymbionts. Here, the genome of SOPE, the obligate mutualistic endosymbiont of rice weevils, was used as a model to analyze the initial events that occur after symbiotic integration. During the first phases of the SOPE genome project, four different types of insertion sequence (IS) elements, belonging to well-characterized IS families from γ-proteobacteria, were identif…

A review of hydropower plants in Romania: Distribution, current knowledge, and their effects on fish in headwater streams

2021

Abstract Hydropower is currently experiencing a boom in southeast Europe. For Romania, the number of hydropower plants is estimated to be between 545 and 674, but little has been published about their environmental impact. We provide the first overview of the geographical distribution of hydropower plants in Romania, supplemented by a review of current knowledge about their environmental impact, and present case study evidence on the effects of small hydropower plants on fish in headwater streams of the Carpathian Mountains. We show that 49% of the documented 545 hydropower plants in Romania are located in Natura 2000 or other protected areas, 5% are located in water bodies with ‘very good’…