Search results for "Fungicides"
showing 10 items of 73 documents
Modelling Inoculum Availability ofPlurivorosphaerella nawaein Persimmon Leaf Litter with Bayesian Beta Regression
2019
AbstractCircular leaf spot (CLS), caused byPlurivorosphaerella nawae, is a serious disease of persimmon (Diospyros kaki) inducing necrotic lesions on leaves, defoliation and fruit drop. Under Mediter-ranean conditions,P. nawaeforms pseudothecia in the leaf litter during winter and ascospores are released in spring infecting susceptible leaves. Persimmon growers are advised to apply fungicides for CLS control during the period of inoculum availability, which was defined based on ascospore counts under the microscope. A model of inoculum availability ofP. nawaewas developed and evaluated as an alternative to ascospore counts. Leaf litter samples were collected weekly in L’Alcúdia from 2010 to…
High osmolarity glycerol (HOG) signalling in Magnaporthe oryzae: Identification of MoYPD1 and its role in osmoregulation, fungicide action, and patho…
2015
AbstractThis study comprises a first functional analysis of an YPD1-homologue in filamentous phytopathogenic fungi and its role in the HOG signalling pathway. We generated a gene deletion mutant of the gene MoYPD1 in Magnaporthe oryzae and characterized the resulting mutant strain. We have shown that MoYpd1p is a component of the phosphorelay system acting in the HOG pathway due to its Y2H protein interaction with the HKs MoHik1p and MoSln1p as well as with the response regulator MoSsk1p. Fungicidal activity of fludioxonil was reported to be based on the inhibition of MoHik1p resulting in hyperactivation of the HOG signalling pathway and lethality. Western analysis proved that both, osmotic…
In vitro mammalian metabolism of the mitosis inhibitor zoxamide and the relationship to its in vitro toxicity.
2009
The in vitro mammalian metabolism of the fungicide zoxamide is related to its in vitro mammalian toxicity. After incubation of zoxamide with rat liver microsomes leading to practically 100% metabolism (mostly hydroxylated zoxamide), the cytotoxicity (methyl thiazole tetrazolium (MTT) test) and the mitosis-inhibiting potential (shown by cell count and by cell cycle analysis) for V79 were not distinguishable from those of zoxamide, demonstrating that the hydroxylation of zoxamide did not change the cytotoxicity or mitosis-inhibiting potential as determined by these assays. After incubation of zoxamide with rat liver S9 predominantly leading to conjugation with glutathione, and after incubatio…
Assessment of chronic effects of tebuconazole on survival, reproduction and growth of Daphnia magna after different exposure times
2015
The effect of the fungicide tebuconazole (0.41, 0.52, 0.71 and 1.14mg/L) on survival, reproduction and growth of Daphnia magna organisms was monitored using 14 and 21 days exposure tests. A third experiment was performed by exposing D. magna to the fungicide for 14 days followed by 7 days of recovery (14+7). In order to test fungicide effects on D. magna, parameters as survival, mean whole body length, mean total number of neonates per female, mean number of broods per female, mean brood size per female, time to first brood/reproduction and intrinsic rate of natural increase (r) were used. Reproduction was seriously affected by tebuconazole. All tebuconazole concentrations tested affected t…
Measured and Modeled Residue Dynamics of Famoxadone and Oxathiapiprolin in Tomato Fields
2018
A reliable analytical method for the simultaneous determination of famoxadone and oxathiapiprolin dissipation kinetics as well as the metabolites of oxathiapiprolin (IN-E8S72 and IN-WR791) in tomato and soil was developed. We studied the dissipation of famoxadone and oxathiapiprolin in tomatoes grown using different kinetic curves in the area of Beijing in 2015 and 2016. Our results show that the most suitable model for two fungicides in 2015 and 2016 was first-order kinetic and second-order kinetic with the half-lives of 3.4 to 5.2 and 2.4 to 3.0 days, respectively. In addition, we applied the dynamic plant uptake model dynamiCROP and combined it with results from the field experiments to …
Pesticide contamination in water and sediment of the aquatic systems of the Natural Park of the Albufera of Valencia (Spain) during the rice cultivat…
2021
The Natural Park of the Albufera (Valencia, Spain) is a Mediterranean wetland where rice cultivation dominates the agricultural activity. The purpose of this study was to offer broader information about the current state of the wetland assessing the contamination by pesticides in four aquatic habitats during the rice cultivation period in 2016. Liquid chromatography with tandem mass spectrometer (UHPLC-MS/MS) was used to determine the pesticides in water and sediment samples from the rice fields, Lake Albufera and irrigation and outlet channels. 21 pesticides were detected in our samples (seven already forbidden by European legislation). Higher values than 10000 ng L of accumulated pesticid…
Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts
2015
Cyprodinil is among the most common agrochemical residues found in highly perishable fruits, such as strawberries. In the present study, high-affinity monoclonal antibodies to this anilinopyrimidine fungicide were raised for the first time with the aim to produce valuable immunochemical analytical assays. Cyprodinil bioconjugates and the generated novel monoclonal antibodies were employed for sensitive competitive immunoassay development in two different formats. The limits of detection of the optimized assays were 20 and 30 ng L-1 for the indirect and direct assay, respectively. Influence over assay parameters of different physicochemical factors was studied. Strawberry samples were extrac…
Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing
2014
4 pages, 1 table, 2 figures.
Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer.
2010
Abstract This paper describes the original synthesis of a functionalized derivative of the fungicide picoxystrobin and the generation of the first reported monoclonal antibodies against this strobilurin pesticide. The synthetic hapten was prepared by total synthesis from commercial chemicals and incorporating the spacer arm through a carbon–carbon single bond. Also, to obtain the immunogen, an uncommon hapten activation strategy based on N,N′-disuccinimidyl carbonate was employed, affording high activation yields and clean and reproducible coupling results. With these immunoreagents, two enzyme-linked immunosorbent assays (ELISAs) were developed: a competitive one-step assay using the antib…
Rational design of a fluopyram hapten and preparation of bioconjugates and antibodies for immunoanalysis
2015
A fluopyram hapten was designed in which insignificant electronic and structural modifications were foreseen and all potentially interacting chemical moieties were maintained. This hapten was prepared by total synthesis and three immunologically active bioconjugates were obtained and characterized. High-affinity and specific antibodies to fluopyram were raised.