Search results for "GD"
showing 10 items of 1142 documents
How school nurses experience and understand everyday pain among adolescents
2017
Abstract Background Pain problems are a rapidly growing health problem found among both children and adolescent, and about 15–30% have reported chronic pain problems. School nurses in Norway meet adolescents with various ailments, including pain. Yet research on how school nurses perceive the pain experienced by adolescents is limited. The aim of the present study was to explore how school nurses explain and experience the everyday pain of adolescents. Method A qualitative study with an explorative design comprising five focus group interviews. Each group consisted of three to five school nurses. Seventeen female school nurses in five junior high schools in Norway, age range 29–65 years par…
αv-Class integrin binding to fibronectin is solely mediated by RGD and unaffected by an RGE mutation.
2020
Fibronectin (FN) is an essential glycoprotein of the extracellular matrix; binds integrins, syndecans, collagens, and growth factors; and is assembled by cells into complex fibrillar networks. The RGD motif in FN facilitates cell binding and fibrillogenesis through binding to α5β1 and αv-class integrins. However, whether RGD is the sole binding site for αv-class integrins is unclear. Most notably, substituting aspartate with glutamate (RGE) was shown to eliminate integrin binding in vitro, while mouse genetics revealed that FNRGE preserves αv-class integrin binding and fibrillogenesis. To address this conflict, we employed single-cell force spectroscopy, engineered cells, and RGD motif–defi…
Amygdalo-hypothalamic projections in the lizardPodarcis hispanica: A combined anterograde and retrograde tracing study
1997
The cells of origin and terminal fields of the amygdalo-hypothalamic projections in the lizard Podarcis hispanica were determined by using the anterograde and retrograde transport of the tracers, biotinylated dextran amine and horseradish peroxidase. The resulting labeling indicated that there was a small projection to the preoptic hypothalamus, that arose from the vomeronasal amygdaloid nuclei (nucleus sphericus and nucleus of the accessory olfactory tract), and an important projection to the rest of the hypothalamus, that was formed by three components: medial, lateral, and ventral. The medial projection originated mainly in the dorsal amygdaloid division (posterior dorsal ventricular rid…
Microremains from El Mirón Cave human dental calculus suggest a mixed plant/animal subsistence economy during the Magdalenian in Northern Iberia
2015
Abstract Despite more than a century of detailed investigation of the Magdalenian period in Northern Iberia, our understanding of the diets during this period is limited. Methodologies for the reconstruction of Late Glacial subsistence strategies have overwhelmingly targeted animal exploitation, thus revealing only a portion of the dietary spectrum. Retrieving food debris from calculus offers a means to provide missing information on other components of diet. We undertook analysis of human dental calculus samples from Magdalenian individuals (including the “Red Lady”) at El Miron Cave (Cantabria, Spain), as well as several control samples, to better understand the less visible dietary compo…
Borrelia burgdorferi ārējās virsmas proteīnu BB0689 un BBA73 funkcionālie pētījumi
2015
Maģistra darbs veidots ar mērķi noskaidrot B. burgdorferi ārējās virsmas proteīnu BB0689 un BBA73 potenciālo lomu patogēna un saimniekorganisma mijiedarbībā, kas varētu paplašināt mūsu zināšanas par Laimboreliozes patoģenēzi. Borēlijas proteīnu un zīdītāju vai artropodu proteīnu iespējamā mijiedarbība ir noteikta, izmantojot Ni-NTA afinitātes hromatogrāfiju, proteīnu kompleksu imūnprecipitāciju (Co-Ip) un attālināto Western blotu. Konstatēto mijiedarbības partneru identifikācijai ir izmantota MALDI-TOF masas spektrometrija. Pētījuma rezultātā ir konstatēta BB0689 iespējamā saistība ar vairākiem zīdītāju proteīniem, savukārt BBA73 gadījumā ir novērota tā mijiedarbība ar Ixodes ērču proteīnie…
Laimas slimības ierosinātāja Borrelia burgdorferi ārējās virsmas proteīna BB0663 iegūšana un struktūras analīze
2019
Darba mērķis bija iegūt Laimas slimības izraisītāja Borrelia burgdorferi (B. burgdorgeri) celma B31 ārējās virsmas proteīna BB0663 struktūru un veikt šīs struktūras analīzi, lai iegūtu plašākas zināšanas par baktērijas patoģenēzes mehānismiem. Darba rezultātā BB0663 proteīns tika uzproducēts Escherichia coli šūnās, attīrīts un kristalizēts. Ar rengenstruktūranalīzes palīdzību tika iegūti šī kristāla difrakcijas dati. Izmantojot iegūtos datus, tika noteikta BB0663 proteīna 3D struktūra. Struktūras analīze parādīja, ka B. burgdorferi BB0663 proteīns ir līdzīgs baktērijas Novosphingobium aromaticivorans (N. aromaticivorans) proteīnam, kuram ir noteikta struktūra, bet nav zināma funkcija. Pētīj…
Unraveling the pleomorphic forms of Borrelia burgdorferi
2014
Borrelia burgdorferi bakteeri on puutiaisten välittämän borrelioosin taudinaiheuttaja. Tämä tavanomaisesti spirokeettana esiintyvä bakteeri kykenee vaihtamaan muotoaan ympäristöolosuhteiden muuttuessa epäsuotuisiksi. Borrelia-bakteerin eri esiintymismuotojen uskotaan olevan borrelioosin pitkittyneen taudinkuvan takana, minkä vuoksi näiden muotojen tutkiminen on tärkeää. Tämän pro gradu –työn tavoitteena oli tutkia B. burgdorferin pleomorfisia muotoja ja löytää muotoja erottavia morfologisia tekijöitä sekä eroja proteiiniekspressiossa ja borrelioosipotilasseerumivasteessa spirokeettojen ja pyöreän esiintymismuodon välillä. Olettamuksena oli, että konfokaalimikroskopialla muotojen väliltä löy…
Bipolar disorder: A neural network perspective on a disorder of emotion and motivation
2013
Bipolar disorder (BD) is a severe, chronic disease with a heritability of 60-80%. BD is frequently misdiagnosed due to phenomenological overlap with other psychopathologies, an important issue that calls for the identification of biological and psychological vulnerability and disease markers. Altered structural and functional connectivity, mainly between limbic and prefrontal brain areas, have been proposed to underlie emotional and motivational dysregulation in BD and might represent relevant vulnerability and disease markers. In the present laboratory review we discuss functional and structural neuroimaging findings on emotional and motivational dysregulation from our research group in BD…
CARATTERIZZAZIONE FENOTIPICA E FUNZIONALE DELLE CELLULE B (CD19+), PLASMACELLULE (CD38+++) DOPPIO NEGATIVE (IgD-CD27-) SU SANGUE MIDOLLARE NEI PAZIEN…
2014
How far are we from the valorization of waste-CO2 to formic acid via electrochemical routes for the applicative scale?
To date, the decarbonization of the world economy and storage and production of energy from alternative C-based sources is considered a relevant topic. The electrochemical conversion of CO2 has been widely investigated since the 1870s as a promising strategy to convert waste-CO2 into value-added chemicals [1,2]. Recently, it was highlighted that, among the several emerging technologies for CO2 conversion to value-added products on an applicative scale, electrochemical technologies are the closest to commercialization due to the numerous start-ups and established companies being invested in this area (e.g., Opus-12, Dioxide Material, and Carbon Recycling International) [3]. These technologie…