Search results for "GRI"

Zinc Binding Sites Conserved in Short Neuropeptides Containing a Diphenylalanine Motif

2019

A diphenylalanine motif in peptides plays a crucial role in supramolecular systems. The current work represents a novel strategy in which a diphenylalanine motif in the central domain of neuropeptides conserves the specific Zn2+ binding site and prevents "hopping" of the Zn2+ ion between alternative metal binding sites. Alternative metal binding sites may also include carboxylic atoms in the terminal domains of a peptide. Therefore, one needs to design a peptide in which the metal will not bind the carboxylic groups in the terminal domains. Herein, we propose that engineering and designing peptides with a diphenylalanine motif in the central domain may yield excellent metal chelators.

Effetti della simbiosi micorrizica su crescita, produttività ed efficienza d’uso dell’azoto in frumento duro (Triticum durum Desf.) in ambiente medit…

Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad

2018

Nowadays, instrumental methodologies and rapid bioanalytical techniques complement each other for the analysis of toxic chemical compounds. Fluxapyroxad was commercialized a few years ago as a fungicide and today it is being used worldwide to control a variety of pests. In the present study, the development of monoclonal antibody-based immunochemical methods for the analysis of this chemical in food samples was evaluated for the first time. Novel haptens were synthesized and protein bioconjugates were prepared. High-affinity and specific monoclonal antibodies to fluxapyroxad were generated from two haptens with alternative linker tethering sites. Haptens with linker site heterology and a st…

Leachate formation and characteristics from gasification and grate incineration bottom ash under landfill conditions

2011

Characteristics and formation of leachates from waste gasification and grate firing bottom ash were studied using continuous field measurements from 112 m(3) lysimeters embedded into landfill body for three years. In addition, the total element concentrations of the fresh ash were analysed and laboratory batch tests were performed to study leachate composition. The three-year continuous flow measurement showed that about one fifth of the leachates were formed, when the flow rate was200 l/d, covering3.5% of the study time. After three years, the liquid/solid-ratio for the quenched grate ash was 1 (l/kg (d.m.)) and for the initially dry gasification ash 0.4 (l/kg (d.m.)). The low initial wate…

Cassava starch processing at small scale in North Vietnam

2008

International audience; In Northern Vietnam, small-scale cassava starch processing is conducted in densely populated craft villages, where processors face difficulties to expand their activities. Three different processing systems were studied among a cluster of three communes in the Red River Delta, producing up to 430 t of starch (at 55% dry matter) per day. The first system, type A, is a cylindrical rasper and a manual sieve, the second, type B, is a cylindrical rasper and stirring-filtering machine and the third, type C, used equipment for both the rasping and filtering stages. Moisture, starch, crude fibers and ash content analysis were carried out on samples collected from the A-B-C m…

Correction: Salinity and Bacterial Diversity: To What Extent Does the Concentration of Salt Affect the Bacterial Community in a Saline Soil?

2014

Three authors were omitted from the author contributions section in the ‘‘contributed to the writing of the manuscript’’ subsection. Please refer to the correct author contributions here: Conceived and designed the experiments: LC GB AB. Performed the experiments: LC GB GLP. Analyzed the data: LC GB FP GLP. Contributed reagents/materials/analysis tools: CD AB GLP. Contributed to the writing of the manuscript: LC FP GB.GLP CD AB

The Application of the Essential Oils of Thymus vulgaris L. and Crithmum maritimum L. as Biocidal on Two Tholu Bommalu Indian Leather Puppets

2021

The chemical profile of the Thymus vulgaris (Lamiaceae) essential oil (EO) was investigated in order to evaluate its biological properties against microorganisms affecting two Tholu Bommalu, typical Indian leather puppets stored at the International Puppets Museum “Antonio Pasqualino” of Palermo, Italy. A GC–MS analysis, using both polar and apolar columns, was used to determine the chemical composition of the essential oil. The aim of this study was to evaluate the antimicrobial effectiveness of the Thymus vulgaris and Crithmum maritimum essential oils in vapor phase to disinfect heritage leather puppets. Pieces of leather artifacts that were affected by different bacterial colonies were e…

Novel antimicrobial ultrathin structures of zein/chitosan blends obtained by electrospinning

2009

Abstract This paper describes the formulation, morphology and biocide properties of novel antimicrobial electrospun zein based ultrathin fiber structures. From the results, it was found that the electrospun fibers of zein can turn the material into a new strong antimicrobial ultrathin-structured system due to retention of remnant amounts of trifluoroacetic acid as determined by ATR–FTIR spectroscopy. Unfortunately, this system may be considered to yield very aggressive high acidic media due to release of the strong acid, which causes the antimicrobial behavior. Nevertheless, since biocide properties are more desirable at mild acidic conditions, blending zein with the natural antimicrobial c…

PEG-benzofulvene copolymer hydrogels for antibody delivery.

2010

A new amphiphilic copolymer have been synthesized starting from the hydrosoluble polyaspartylhydrazide (PAHy) polymer, by grafting both hydrophilic PEG(2000) chains and hydrophobic palmitic acid (C(16)) moieties on polymer backbone, and the structure of obtained PAHy-PEG(2000)-C(16) copolymer have been characterized by 2D (1)H/(13)C NMR experiments. PAHy-PEG(2000)-C(16) copolymer showed the ability of self-assembling in aqueous media giving a core-shell structure and resulted potentially useful for encapsulating and dissolving hydrophobic drug. The formation of micellar core-shell structure has been investigated by 2D (1)H NMR NOESY experiments. The presence of cross-peaks for protons of C(…

Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

2020

Organ-on-chip (OOC) devices are miniaturized devices replacing animal models in drug discovery and toxicology studies. The majority of OOC devices are made from polydimethylsiloxane (PDMS), an elastomer widely used in microfluidic prototyping, but posing a number of challenges to experimentalists, including leaching of uncured oligomers and uncontrolled absorption of small compounds. Here we assess the suitability of polylactic acid (PLA) as a replacement material to PDMS for microfluidic cell culture and OOC applications. We changed the wettability of PLA substrates and demonstrated the functionalization method to be stable over a time period of at least 9 months. We successfully cultured …