Search results for "Gate"

Analysis of phosphorylation-dependent modulation of Kv1.1 potassium channels.

2003

The voltage-gated potassium channel Kv1.1 contains phosphorylation sites for protein kinase A (PKA) and protein kinase C (PKC). To study Kv1.1 protein expression and cellular distribution in regard to its level of phosphorylation, the effects of PKA and PKC activation on Kv1.1 were investigated in HEK 293 cells stably transfected with Kv1.1 (HEK 293/1). Without kinase activation, HEK 293/1 cells carry unphosphorylated Kv1.1 protein in the plasma membranes, whereas large amounts of phosphorylated and unphosphorylated Kv1.1 protein were located intracellularly. Activation of PKA resulted in phosphorylation of intracellular Kv1.1 protein, followed by a rapid translocation of Kv1.1 into the pla…

Sequential acquisition of cacophony calcium currents, sodium channels and voltage-dependent potassium currents affects spike shape and dendrite growt…

2014

During metamorphosis the CNS undergoes profound changes to accommodate the switch from larval to adult behaviors. In Drosophila and other holometabolous insects, adult neurons differentiate either from respecified larval neurons, newly born neurons, or are born embryonically but remain developmentally arrested until differentiation during pupal life. This study addresses the latter in the identified Drosophila flight motoneuron 5. In situ patch-clamp recordings, intracellular dye fills and immunocytochemistry address the interplay between dendritic shape, excitability and ionic current development. During pupal life, changes in excitability and spike shape correspond to a stereotyped, progr…

Determination of Human Granulocyte Elastase by the Immunoactivation Method on the Hitachi® 717 Automated Analyser

1991

This paper describes a fully mechanized homogeneous immunoassay using the immunoactivation method for the rapid and specific determination of human granulocyte elastase (EC 3.4.21.37) in plasma. The method uses anti-elastase antibody fragments from sheep, conjugated to horseradish peroxidase. These enzyme-antibody conjugates bind to the elastase-alpha 1-proteinase inhibitor complex present in plasma. A separate sample blank with non-specific sheep antibody fragments conjugated to horseradish peroxidase corrects for errors introduced by the sample matrix. Measurements were performed with the clinical chemistry analyser Hitachi 717. A single determination can be performed in 10 min, requiring…

Pneumococcal conjugate vaccines in Europe, Berlin, Germany, 23–25 August 2000. Report of a European Advisory Board meeting

2001

Immunogenicity, reactogenicity and safety of a 7-valent pneumococcal conjugate vaccine (PCV7) concurrently administered with a DTPa-HBV-IPV/Hib combi…

2006

Abstract Background To evaluate immunogenicity, reactogenicity, and safety of a hexavalent combination vaccine diphtheria-tetanus-acellular pertussis-hepatitis B-inactivated polio virus- Haemophilus influenzae type b (DTPa-HBV-IPV/Hib) when coadministered with a 7-valent pneumococcal conjugate vaccine (PCV7). Methods Infants received either a hexavalent diphtheria-tetanus-acellular pertussis-hepatitis B-inactivated polio virus- H. influenzae type b vaccine concomitantly with PCV7 or DTPa-HBV-IPV/Hib alone infants were vaccinated at 2, 3 and 4 months (primary immunization) and 12–15 months of age (booster dose). Local and systemic reactions and adverse events were monitored following each do…

The potential for pneumococcal vaccination in Hajj pilgrims: expert opinion.

2013

Hajj is the annual pilgrimage to Mecca in the Kingdom of Saudi Arabia, and is one of the largest mass gathering events in the world. Acute respiratory tract infections are very common during Hajj, primarily as a result of close contact among pilgrims, intense congestion, shared accommodation and air pollution. A number of vaccines are (or have been) recommended for Hajj pilgrims in recent years. Several additional vaccines could significantly reduce the morbidity and mortality at Hajj and should be considered in health recommendations for pilgrims. Pneumococcal vaccines (particularly for those aged >65 years) are widely available, and have been shown to reduce the burden of disease associat…

Safety and reactogenicity of a novel DTPa-HBV-IPV combined vaccine given along with commercial Hib vaccines in comparison with separate concomitant a…

2002

Objective. Combination vaccines simplify vaccine administration and have the potential to promote compliance and cost-effectiveness by decreasing the number of injections needed to immunize a child. The objective of this study was to assess the safety and reactogenicity of the diphtheria-tetanus toxoid-acellular pertussis-hepatitis B virus-inactivated polio virus (DTPa-HBV-IPV) vaccine when coadministered with different Haemophilus influenzae type B (Hib) vaccines in comparison with separate, commercially available, control vaccines in a 3-dose primary vaccination series. Methods. An open-label, randomized, parallel-group study in 5318 infants who were 8 to 16 weeks of age at enrollment was…

How does the journal impact factor affect the CV of PhD students?

2014

In his editorial “Dear DORA”, Howy Jacobs commented on the recent San Francisco Declaration of Research Assessment (DORA) to address the misuse of the journal impact factor (IF) and discussed alternatives. DORA stipulates that the IF must not be used as a surrogate measure of the quality of individual research articles, or to assess an individual scientist's contributions in hiring, promotion and funding decisions. DORA and many other commentators, such as Howy Jacobs, therefore advocate the use of additional, alternative metrics and other measures to make a more fair and realistic judgment about the quality of a scientist's or …

Cryotherapy, Hydrotherapy and other training recovery modalities: Effects on hormones’ balance and performance

2010

Recovery modalities (RM) are an important aspect of training programmes, providing physiological benefits to athletes and enamelling them a homeostasis balance between training and competitions. Recovery allows the athlete to return to the state of performance readiness by restoring all the physiological parameters back to baseline levels (Barnett 2006, Reilly and Ekblom 2005, Gill et al. 2006). RMs have been largely investigated in regards to their ability to enhance blood lactate removal or to reduce exercise induced delayed onset of muscle soreness (DOMS) (Barnett 2006). The endogenous hormones influence the body’s adaptation to stress stimulus, therefore, investigating these hormones du…

Differentiation of an additive interval measure with values in a conjugate Banach space

2014

We present a complete characterization of finitely additive interval measures with values in conjugate Banach spaces which can be represented as Henstock-Kurzweil-Gelfand integrals. If the range space has the weak Radon-Nikodým property (WRNP), then we precisely describe when these integrals are in fact Henstock-Kurzweil-Pettis integrals.