Search results for "Gel"

Barthowskie spekulacje angelologiczne w "Die kirchliche Dogmatik" w świetle katolickich wypowiedzi dogmatycznych o aniołach

2015

Zasadniczą problematyką artykułu jest analiza wykładni angelologicznej dokonana przez Karla Bartha w jego monumentalnym dziele Dogmatyka kościelna i porównanie jej z katolicką ortodoksją Urzędu Nauczycielskiego Kościoła. Celem jest obrona tezy, że nauka o aniołach – tylko o tych, które nie upadły – zarysowana przez kontrowersyjnego, ale też znaczącego protestanckiego teologa, w żadnym wypadku nie musi stać w sprzeczności z powściągliwymi i mało precyzyjnymi stwierdzeniami odnośnie do istot anielskich, które przez Kościół rzymskokatolicki uznane zostały za wypowiedzi dogmatyczne. W pierwszej części artykułu został przedstawiony historyczny rozwój angelologii w oparciu o retrospektywne interp…

La potenza del vuoto

2011

Role of Seroalbumin in the Cytotoxicity of cis-Dichloro Pt(II) Complexes with (N^N)-Donor Ligands Bearing Functionalized Tails

2018

Given the potent anticancer properties of cisdiamminedichloroplatinum( II) and knowing its mode of action, we synthesized four new cis-[PtCl2(N^N)] organoplatinum complexes, two with N-substituted pbi ligands (pbiR = 1-R-2-(2-pyridyl)benzimidazole) (namely, 1 and 2) and two more with 4,4′-disubstituted bpy ligands (bpy = 2,2′-bipyridine) (namely, 3 and 4). We explored their cytotoxicity and ability to bind to deoxyguanosine monophosphate (dGMP), DNA, and albumin models. By 1H NMR and UV−vis spectroscopies, circular dichroism, agarose gel electrophoresis, differential scanning calorimetry measurements, and density functional theory calculations, we verified that only 3 can form aquacomplex s…

Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing.

2021

Abstract Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low c…

Thermal aggregation and ion-induced cold-gelation of bovine serum albumin

2009

Protein cold-gelation has recently received particular attention for its relevance in bio and food technology. In this work, we report a study on bovine serum albumin cold-gelation induced by copper or zinc ions. Metal-induced cold-gelation of proteins requires two steps: during the first one, the heat treatment causes protein partial unfolding and aggregation; then, after cooling the solution to room temperature, gels are formed upon the addition of metal ions. The thermally induced behaviour has been mainly investigated through different techniques: Fourier transform infrared (FTIR) spectroscopy, circular dichroism, dynamic light scattering (DLS) and rheology. Data have shown that the agg…

Ferricytochrome c encapsulated in silica nanoparticles: structural stability and functional properties.

2004

Using a modified sol-gel technique, we have succeeded in encapsulating ferric cytochrome c in silica nanoparticles obtained from hydrolysis and polycondensation of tetramethylorthosilicate. Particles dimensions have been determined with dynamic light scattering; this technique yields an hydrodynamic radius of about 100 nm, each nanoparticle containing about 10(2)-10(3) proteins. If stored in the cold at low ionic strength, nanoparticles are stable for more than one week, even if a slow radius increase with time is observed. CD measurements show that encapsulated proteins exhibit substantially increased stability against guanidinium hydrochloride induced denaturation. Reduction kinetics of e…

Size-exclusion high-performance liquid chromatography in the study of the autoassociating antibiotic gramicidin A in micellar milieu.

2003

Gramicidin A (gA) is a polypeptide antibiotic which forms dimeric channels specific for monovalent cations in biological membranes. It is a polymorphic molecule that adopts several different conformations, double-stranded (ds) helical dimers (pore conformation) and single-stranded beta-helical dimers (channel conformation). This study investigated the conformational adaptability of gramicidin A when incorporated into micelles as membrane-mimetic model system. Taking advantage of our reported, versatile, size-exclusion high-performance liquid chromatography (SE-HPLC) strategy that allows the separation of double-stranded dimers and monomers, we have quantitatively characterized the conformat…

Isolation and characterization of haemoporin, an abundant haemolymph protein from Aplysia californica.

2003

In the present study, we show the isolation and characterization of the protein haemoporin, which constitutes the second most abundant protein fraction in the haemolymph of the marine gastropod Aplysia californica. Although Aplysia is commonly used to investigate the molecular basis of learning, not much is known about the proteins in its haemolymph, which is in contact with the neurons owing to the open circulatory system of molluscs. In the native state, haemoporin is a macromolecular complex forming a cylinder with a central solvent-filled pore. The native complex most probably is a homopentamer made up from 70 kDa subunits with a molecular mass of 360 kDa and a sedimentation coefficient…

Solving Rate of Change Tasks with a Graphing Calculator: a Case Study on Instrumental Genesis

2016

In an increasing number of mathematics classes throughout the world, technology is being used for the teaching and learning of mathematics. But knowledge is limited about the long-term development of students’ mathematical thinking when learning mathematics with the use of technology. This article reports on the development of a student and the role of the graphing calculator (GC) in his learning about derivatives and instantaneous rate of change. This case is compelling, because the student is an intensive user of the GC and develops flexible problem-solving techniques – techniques which differ from those of his peers and from what he was taught in mathematics class. We used the framework …

The linearized Calderón problem on complex manifolds

2019

International audience; In this note we show that on any compact subdomain of a Kähler manifold that admits sufficiently many global holomorphic functions , the products of harmonic functions form a complete set. This gives a positive answer to the linearized anisotropic Calderón problem on a class of complex manifolds that includes compact subdomains of Stein manifolds and sufficiently small subdomains of Kähler manifolds. Some of these manifolds do not admit limiting Carleman weights, and thus cannot by treated by standard methods for the Calderón problem in higher dimensions. The argument is based on constructing Morse holo-morphic functions with approximately prescribed critical points.…