Search results for "Gels"
showing 10 items of 671 documents
Ocular gelling microspheres: in vitro precorneal retention time and drug permeation through reconstituted corneal epithelium.
2008
Purpose: The model drug norfloxacin (NOR)was encapsulated into trehalose (TRH) and hydroxyethylcellulose(NAT) microspheres to obtain a novel gelling ophthalmic delivery system for prolonged release on corneal tissue. Methods: We assessed NOR release from microspheres, prepared by the emulsion-solvent evaporation method. A new in vitro tear turnover model, including inserts containing reconstituted human corneal epithelium (RHC), was designed to evaluate the TRH/NAT microspheres’ precorneal retention time. Bioadhesive properties of TRH/NAT microspheres were validated by using drug-loaded microspheres prepared with gelatine (GLT) commonly used as reference material in adhesion studies. Result…
A new method to value efficiency of enzyme blends for pancreatic tissue digestion.
2010
Islet transplantation, since the 90’s, has been resulting to be one of the best successful example of human cell therapy. Nevertheless, islet isolation procedure is not completely standardized; in fact, more than fifty percent of islets procedures don’t arrive to their transplantation. This is due both to the variability of donor’s pancreas and to an unpredictable enzymatic blend efficiency. Enzymes used in pancreas digestion are extracted from Clostridium histolyticum bacteria and digest several substrates. In particular they have strong collagenolytic activity compared to vertebrate collagenases. However, several impediments persist in human islet isolation success probably due to the var…
Lexikal transfer från engelska i högstadieelevers och gymnasisters texter samt svensklärares attityder till engelska i det svenska språket
2016
Tutkielman tarkoituksena oli kartoittaa, miten englannin kielen vaikutus näkyy yläasteikäisten ja lukioikäisten kirjoittamissa ruotsinkielisissä teksteissä leksikaalisella tasolla. Lisäksi tutki-muksessa tarkasteltiin ruotsinopettajien asenteita englannin kielen vaikutusta kohtaan ruotsin kielessä. Opettajien asenteita tutkittiin nettikyselyn avulla ja kyselyyn osallistui yhteensä 156 opettajaa. Oppilastekstit valittiin A1- ja A2-tasoilta ja tekstejä oli yhteensä 115. Tutkimustulok-set analysoitiin sekä kvantitatiivisin että kvalitatiivisin menetelmin. Tutkimuksen tulokset osoittavat, että englannin vaikutus näkyy eniten lainasanojen käytössä sekä sanoissa, joissa yhdistyy piirteitä sekä ru…
Marx y Engels y la división sexual del trabajo en la familia: ambigüedades, equívocos y vacíos teóricos
2018
Dada la importante influencia de Marx y Engels en el análisis de la estructura social, no sorprende el notable impacto de su obra en el estudio del papel de la mujer en la familia. Sin embargo, su herencia en este ámbito sociológico ha dado lugar a interpretaciones muy polarizadas. Este artículo quiere aportar algo de luz evidenciando las contradicciones del marxismo al estudiar el papel de la mujer en la familia. Para ello se comparan las contribuciones de Marx y Engels para calibrar posibles diferencias en las posturas de dos autores que en otros ámbitos han presentado un pensamiento muy homogéneo. Los resultados obtenidos permiten afirmar que, a pesar de su innegable influencia en el pen…
Systems level approach reveals the correlation of endoderm differentiation of mouse embryonic stem cells with specific microstructural cues of fibrin…
2014
Stem cells receive numerous cues from their associated substrate that help to govern their behaviour. However, identification of influential substrate characteristics poses difficulties because of their complex nature. In this study, we developed an integrated experimental and systems level modelling approach to investigate and identify specific substrate features influencing differentiation of mouse embryonic stem cells (mESCs) on a model fibrous substrate, fibrin. We synthesized a range of fibrin gels by varying fibrinogen and thrombin concentrations, which led to a range of substrate stiffness and microstructure. mESCs were cultured on each of these gels, and characterization of the diff…
Fluorescent naphthalimide-imidazolium hydrogels for biomedical applications
2019
Bioimaging and in vivo imaging are cornerstone technologies in support of biomedical diagnosis. However, in some cases imaging methods have increased cancer risks for patients. Moreover, the most widely used diagnostic medical imaging technique, X-ray imaging, is the largest man-made source of radiation exposure to the general population. Thus, the research of new efficient and less invasive materials for imaging is quite urgent. Supramolecular hydrogels have recently proved to be promising biological carriers to load versatile bioimaging agents for in vitro or in vivo bioimaging, thanks to the ability to undergo reversible swelling and gel–sol transition in response to various physiologica…
Modular Small Diameter Vascular Grafts with Bioactive Functionalities.
2015
We report the fabrication of a novel type of artificial small diameter blood vessels, termed biomimetic tissue-engineered blood vessels (bTEBV), with a modular composition. They are composed of a hydrogel scaffold consisting of two negatively charged natural polymers, alginate and a modified chitosan, N,O-carboxymethyl chitosan (N,O-CMC). Into this biologically inert scaffold two biofunctionally active biopolymers are embedded, inorganic polyphosphate (polyP) and silica, as well as gelatin which exposes the cell recognition signal, Arg-Gly-Asp (RGD). These materials can be hardened by exposure to Ca(2+) through formation of Ca(2+) bridges between the polyanions, alginate, N,O-CMC, and polyP…
Viscoelastic properties of acid milk gel as affected by fat nature at low level
2005
International audience; The viscoelastic properties of acid milk gels containing small amounts of different fats were investigated. Skim milk was reconstituted from ultra low-heat skim milk powder and emulsions made with 2% (v/v) sunflower oil, olive oil, groundnut oil, or anhydrous milk fat using a pressure homogenizer. Acidification at 20 °C for 14 h to pH ∼4.6 was achieved by adding glucono-δ-lactone to the emulsion. Stress relaxation testing enabled determination of the firmness and the solid-like properties, i.e., elasticity. Regardless of the physical state of the fat, emulsion gels exhibited higher firmness than fat-free gels, despite the low fat level used. The firmness of the gels …
Microstructure and characteristic properties of dogfish skin gelatin gels prepared by freeze/spray-drying methods
2020
International audience; The effects of two pretreatments (microwaves or oven-drying) on the dogfish (Squalus acanthias) skin as well as two drying processes (freeze-drying or spray-drying) on the extracted gelatins were studied. Thus six types of gelatins were obtained, three of which were freeze-dried (FG) and the others were spray-dried (SG), from the untreated skin (US), microwaves-pretreated skin (MS) and oven-pretreated skin (OS). The highest yield (8.67%) was obtained for the OSFG, while the lowest one (3.06%) was measured for the OSSG. Interestingly, all gelatins exhibited relatively high protein (84.02-89.53%), and low lipid (0.50-1.71%) and ash (3.05-7.17%) contents. In addition, g…
Structural behaviour differences in low methoxy pectin solutions in the presence of divalent cations (Ca 2+ and Zn 2+ ): a process driven by the bind…
2014
International audience; In this paper, we compare the interactions between low methoxy pectin (LMP) and either Ca2+ or Zn2+ in semi-dilute solutions. Intrinsic viscosity and turbidity measurements reveal that pectin-calcium solutions are more viscous, but yet less turbid, than pectin-zinc ones. To get a molecular understanding on the origin of this rather unexpected behavior, we further performed isothermal titration calorimetry, small angle neutron scattering experiments, as well as molecular dynamics simulations. Our results suggest that calcium cations induce the formation of a more homogeneous network of pectin than zinc cations do. The molecular dynamics simulations indicate that this …