Search results for "Generation"

Ernährungsphysiologie erhitzter Fette: Langfristige Tierversuche

1974

Das Fette, die bei intensiver Beruhrung mit dem Luftsauerstoff langere Zeit auf hohere Temperaturen erhitzt worden sind, toxische Eigenschaften entwickeln, ist in vielen Laboratorien ubereinstimmend gezeigt worden. Je nach Schwere des Eingriffs reichen die bei Futterungsversuchen erhobenen Befunde von einer praktisch symptomenlosen Vertraglichkeit uber mehr oder minder schwere Wachstumsverzogerungen junger Tiere bis zu einer hohen Toxicitat wie starke Erhohung der Letalitatsrate und schweren anatomischen und histologischen Veranderungen der Organe. Fritierfette haben sich beim Einhalten der folgenden Bedingungen: Temperaturen nicht uber 180° C, Vermeiden jeder lokalen Uberhitzung durch einw…

Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon)

2008

Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l-1 malt extract (MSI), or 500 mg l-1 malt extract and 13.3 ¼M 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic emb…

The role of exercise on peripheral nerve regeneration: from animal model to clinical application

2021

Peripheral nerve injury is a complex condition with a variety of signs and symptoms depending on the severity and nerves involved. Peripheral nerve damage may lead to sensory and motor functions deficits and even lifelong disability, causing important socioeconomic costs worldwide. Despite the increase in knowledge of the mechanisms of injury and regeneration, a full functional recovery is still unsatisfying in the majority of patients. It is well known that exercise promotes physical and psychological well-being, by ameliorating general health. In the last years, there has been a growing interest in evaluating the effects of exercise on the peripheral nervous system. Experimental works wit…

“Evidence of HIV-1 second-generation recombinant strains (SGRs) in Sicily: sequence analysis of gag, pol, and env genes.”

2007

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines

2014

Cancer cell lines are a tremendous resource for cancer biology and therapy development. These multipurpose tools are commonly used to examine the genetic origin of cancers, to identify potential novel tumor targets, such as tumor antigens for vaccine devel-opment, and utilized to screen potential therapies in preclinical studies. Mutations, gene expression, and drug sensitivity have been determined for many cell lines using next-generation sequencing (NGS). However, the human leukocyte antigen (HLA) type and HLA expression of tumor cell lines, characterizations necessary for the development of cancer vaccines, have remained largely incomplete and, such information, when available, has been …

Activation of a caspase-3-independent mode of cell death associated with lysosomal destabilization in cultured human retinal pigment epithelial cells…

2008

International audience; Purpose: To characterize the possible cytotoxic effects of oxysterols (7-hydroxycholesterol (7-OH), 25-hydroxycholesterol (25-OH)) in human retinal pigment epithelial cells (ARPE-19) and to detail the relationships between some of these effects. Methods: ARPE-19 cells were treated with 7-OH and 25-OH. Cell viability was measured with the MTT assay. Membrane permeability, mitochondrial potential, and lysosomal integrity were measured by flow cytometry with propidium iodide, DiOC6(3), and acridine orange, respectively. Cell death was characterized by staining with Hoechst 33342, transmission electron microscopy, and analysis of the DNA fragmentation pattern. Caspase ac…

Elementary presentation of self‐consistent intermediate Hamiltonians and proposal of two totally dressed singles and doubles configuration interactio…

1994

Intermediate Hamiltonians are effective Hamiltonians which are defined on an N‐dimensional model space but which only provide n<N exact eigenvalues and the projections of the corresponding eigenvectors onto the model space. For a single root research, the intermediate Hamiltonian may be obtained from the restriction of the Hamiltonian to the model space by an appropriate, uniquely defined dressing of the diagonal energies or of the first column. Approximate self‐consistent dressings may be proposed. The simplest perturbative form gives the same result as the original 2nd order intermediate Hamiltonian or the ‘‘shifted Bk’’ technique but it is of easier implementation. Self‐consistent inclus…

Probing the Dynamics of a Molecular Ion with Laser Pulses

2004

The dynamics of a H2+ molecular ion driven by two laser pulses separated by a time lag is studied beyond the Born-Oppenheimer approximation. The first, short, pulse prepares the molecule in some quantum state, which is probed by the second pulse. Under suitable conditions, the molecule emits a spectrum of redshifted high-order harmonics. The value of the redshift is proportional to the harmonic order and can be used as a measure of the speed of the atoms of the molecule.

Microwave Response of Ceramic MgB2 Samples

2009

The microwave response of ceramic MgB2 has been investigated as a function of temperature and external magnetic field by two different techniques: microwave surface impedance and second-harmonic emission measurements. The measurements of the surface resistance have shown that microwave losses in MgB2 are strongly affected by the magnetic field in the whole range of temperatures below Tc, even for relatively low field values. The results have been accounted for in the framework of the Coffey and Clem model hypothesizing that in different temperature ranges the microwave current induces fluxons to move in different regimes. In particular, the results at temperatures close to Tc have been quan…

Alignement moléculaire : caractérisation et application à la mesure de thermalisation ultra-rapide et au contrôle de génération d'harmoniques

2013

The thematic of this thesis is molecular alignment. The latter is a very important topic that opens the way toward a much more thin control of many phenomenons. So, we have developed a new measurement technique of the molecular alignment along one axis that permits to preserve the sign of alignment. This one is, like other measurement techniques developed by the team,based on the measurement of the refractive index variation induced by the molecular alignment.The technique developed then also permits the molecular alignment measurement, being also an application of it because it allows the third harmonic generation. In the last study, molecular alignment is implemented to show that it bring…