Search results for "Gill"

Selected plant essential oils and their main active components, a promising approach to inhibit aflatoxigenic fungi and aflatoxin production in food.

2018

Recent research has showed that Aspergillus flavus and Aspergillus parasiticus are aflatoxigenic species that can become very competitive in the framework of climate change. Aflatoxins show carcinogenic, mutagenic, immunotoxic and teratogenic effects on human and animals. Effective and sustainable measures to inhibit these species and aflatoxins in food are required. Origanum vulgare and Cinnamomum zeylanicum essential oils (EOs) and their major active constituents, carvacrol and cinnamaldehyde, respectively, were assayed for inhibiting these species and aflatoxin production in maize extract medium under different environmental conditions. Doses of 10-1000 mg l

Influence of probiotic microorganisms on aflatoxins B 1 and B 2 bioaccessibility evaluated with a simulated gastrointestinal digestion

2018

Aflatoxins (AFs) are produced mainly by the molds Aspergillus flavus and Aspergillus parasiticus. Aflatoxin B1 (AFB1) is classified as carcinogenic to humans. The aim of this study was to evaluate the capacity of different strains of Lactobacilli (Lb.) and Bifidobacteria (Bf.) to reduce the bioaccessibility of AFB1 and aflatoxin B2 (AFB2), spiked in loaf bread, using a dynamic in vitro simulated gastrointestinal digestion system. Aliquots of 20 mL of gastric and duodenal fluids were sampled for the determination of the mycotoxins gastric and duodenal bioaccessibility respectively, by liquid-chromatography coupled to the mass spectrometry in tandem (LC–MS/MS). A reduction of AFs bioacc…

Fumigation of Brazil nuts with allyl isothiocyanate to inhibit the growth of Aspergillus parasiticus and aflatoxin production

2017

Abstract BACKGROUND Brazil produces approximately 40 000 tons of Brazil nuts annually, which is commonly contaminated with fungi and mycotoxins. Gaseous allyl isothiocyanate (AITC) was used to inhibit the growth of Aspergillus parasiticus and its production of aflatoxins (AFs) in Brazil nuts. RESULTS Nuts were inoculated with 104 spores g−1 of A. parasiticus and placed in airtight glass jars with controlled relative humidity (RH = 95 or 85%). Samples were treated with 0, 0.5, 1.0 or 2.5 µL L−1 of gaseous AITC and analyzed after 30 days to determine the fungal population and AFs content. Samples were also submitted to sensory evaluation. AITC at 2.5 µL L−1 could completely inhibit the fungal…

Influence of the antimicrobial compound allyl isothiocyanate against the Aspergillus parasiticus growth and its aflatoxins production in pizza crust.

2015

Abstract Aflatoxins (AFs) are secondary metabolites produced by different species of Aspergillus, such as Aspergillus flavus and Aspergillus parasiticus, which possess mutagenic, teratogenic and carcinogenic activities in humans. In this study, active packaging devices containing allyl isothiocyanate (AITC) or oriental mustard flour (OMF) + water were tested to inhibit the growth of A. parasiticus and AFs production in fresh pizza crust after 30 d. The antimicrobial and anti-aflatoxin activities were compared to a control group (no antimicrobial treatment) and to a group added with commercial preservatives (sorbic acid + sodium propionate). A. parasiticus growth was only inhibited after 30 d…

In vitro antifungal activity of lactic acid bacteria against mycotoxigenic fungi and their application in loaf bread shelf life improvement

2016

Food spoilage caused by mycotoxigenic fungi represents an important food safety problem. Lactic acid bacteria (LAB) are used as starter cultures in a larger number of food products. In this study, 16 strains of LAB were cultivated in MRS broth under anaerobiosis. Then, cell free supernatants were obtained by centrifugation and their antifungal activity against Aspergillus parasiticus and Penicillium expansum was tested using the disc-diffusion method. Furthermore, the LABs that showed in vitro antifungal activity were used in bread fermentation with yeast in order to study fungal growth inhibition and aflatoxin (AF) reduction in processed bread previously inoculated with A. parasiticus. The…

Liberaali välttämättömyys ja paljaan elämän politiikka

2010

Romanization of Funerary Rituals in Oretania: The Tomb 1 of the Cemetery of the Cerro de las Cabezas (Valdepeñas, Ciudad Real, Spain)

2017

La correcta aplicación de un procedimiento de prevención de impactos y protección al patrimonio arqueológico, desarrollado con motivo de la implantación de una línea eléctrica de alta tensión, permitió en 2013 el hallazgo de una necrópolis junto al Cerro de las Cabezas (Valdepeñas, Ciudad Real). Hasta el momento se desconocía la localización del área funeraria de este gran oppidum. Las catorce tumbas documentadas durante esta breve intervención (catorce días de trabajo) corresponden a los enterramientos de cremaciones en urnas, con piezas de ajuar asociadas como fusayolas, fíbulas, armas y recipientes cerámicos. Se presenta el estudio ceramológico de la Tumba 1; la única de entre las excava…

Übertragung von Verhaltensweisen durch Transplantation von Anlagen neuroanatomischer Strukturen bei Amphibienlarven

2010

By replacing parts of the neural plate (prospective medulla oblongata) of Triturus vulgaris by neural ectoderm of Xenopus laevis larval chimaeras were obtained, which showed donorlike rhythmical movements of distinct parts of the visceral musculature according to the place of implantation. Especially the branchial region with its external gills showed rhythmical movements and is the special object of this investigation.

The logarithmic transformation should be avoided for stabilising the variance of mould growth rate.

2007

Abstract Radial growth rate, μ (mm d − 1 ) was evaluated by plotting the radius of the colony, r (mm) versus time (d) for Alternaria alternata , Aspergillus flavus , Cladosporium cladosporioides , Mucor racemosus , Rhizopus oryzae and Trichoderma harzianum at different T and a w . For each of the 12 data sets, an analysis of variance of the raw growth rate data was performed. It was observed from the P -values that all square-root transformed values of μ were non-significant at the significance level α  = 0.05, whereas for untransformed values of μ , three of the 12 values were significant and for logarithmically transformed μ , nine of the 12 values were significant at the significance lev…

Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

2019

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding ca…