6533b86cfe1ef96bd12c82fe

RESEARCH PRODUCT

Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

Denis DontsovAngelina PittnerGünter UhlrichAndré DatheAndré DatheWalter SchottDavid ZopfJ. Jussi ToppariKai ArstilaWolfgang FritzscheAndrea CsákiNorman GrosseOndrej Stranik

subject

AnalyteMaterials scienceta221Molecular bindingMetal NanoparticlesNanoparticleBioengineering02 engineering and technologybiosensorit01 natural sciencesSensor arrayLimit of DetectionNanosensorplasmonic arrayNanotechnologyLSPR sensingSurface plasmon resonanceDNA FungalInstrumentationPlasmonCandidaDNA detectionFluid Flow and Transfer ProcessesPlasmonic nanoparticlesBase Sequenceta114business.industryProcess Chemistry and Technology010401 analytical chemistryta1182Fourier-transform-imaging spectroscopynanobiotekniikkaDNASurface Plasmon Resonance021001 nanoscience & nanotechnologyplasmonic nanoparticles0104 chemical sciencesAspergillusOptoelectronicsnanohiukkasetGoldDNA Probes0210 nano-technologybusiness

description

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding can be reversed using chemical means (10 mM HCl). It is demonstrated that multiplexed detection and identification of several fungal pathogen DNA sequences subsequently on one sensor array are possible by this approach.

yearjournalcountryeditionlanguage
2019-01-19ACS Sensors
https://doi.org/10.1021/acssensors.8b01073