Search results for "Glomerulus"

showing 3 items of 33 documents

Vitamin A deficiency alters the structure and collagen IV composition of rat renal basement membranes.

2005

Retinoids can modulate the expression of extracellular matrix (ECM) proteins with variable results depending on other contributing factors. Because changes in these proteins may alter the composition and impair the function of specialized ECM structures such as basement membranes (BMs), we studied the effects of vitamin A deficiency on renal BMs during the growing period. Newborn male rats were fed a vitamin A-deficient (VAD) diet for 50 d. The ultrastructure of renal BMs was analyzed by electron microscopy. Total collagen IV, the different alpha(IV) chains, matrix degrading metalloproteinases (MMP), and tissue inhibitors of metalloproteinases (TIMP) were quantified by immunocytochemistry a…

VitaminCollagen Type IVmedicine.medical_specialtyMMP2Kidney GlomerulusMedicine (miscellaneous)BiologyMatrix metalloproteinaseMMP9KidneyBasement MembraneExtracellular matrixchemistry.chemical_compoundInternal medicinemedicineAnimalsRNA MessengerRats WistarTIMP1DNA PrimersBasement membraneKidneyNutrition and DieteticsBase SequenceReverse Transcriptase Polymerase Chain ReactionVitamin A DeficiencyMatrix MetalloproteinasesRatsmedicine.anatomical_structureEndocrinologychemistryFemaleThe Journal of nutrition
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Cross-reactivity of anti-ssDNA antibodies with heparan sulfate in patients with type I diabetes mellitus

1989

Anti-single-stranded–DNA antibodies cross-reactive with heparan sulfate were detected in serums of patients with type I (insulin-dependent) diabetes mellitus. The results suggested that heparan sulfate, the major glycosaminoglycan constituent of the glomerular basement membrane, may serve as a target antigen in vivo for cross-reactive anti-DNA antibodies. These polyreactive antibodies, directed toward repeating negatively charged units, may neutralize the heparan sulfate–associated polyanionic sites in the glomerulus, leading to an abnormal permeability of anionic plasma proteins.

medicine.medical_specialtyRenal glomerulusEndocrinology Diabetes and MetabolismDNA Single-StrandedEnzyme-Linked Immunosorbent AssayPerlecanCross ReactionsBiologyGlycosaminoglycanchemistry.chemical_compoundInternal medicinemedicineInternal MedicineHumansChildGlycosaminoglycansBasement membraneGlomerular basement membraneHeparan sulfateBlood proteinsMolecular biologyDiabetes Mellitus Type 1Endocrinologymedicine.anatomical_structurechemistryImmunoglobulin Gbiology.proteinHeparitin SulfateAntibody
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Regulation of glomerular basement membrane collagen expression by LMX1B contributes to renal disease in nail patella syndrome.

2001

Basement membrane (BM) morphogenesis is critical for normal kidney function. Heterotrimeric type IV collagen, composed of different combinations of six alpha-chains (1-6), is a major matrix component of all BMs (ref. 2). Unlike in other BMs, glomerular BM (GBM) contains primarily the alpha 3(IV) and alpha 4(IV) chains, together with the alpha 5(IV) chain. A poorly understood, coordinated temporal and spatial switch in gene expression from ubiquitously expressed alpha 1(IV) and alpha 2(IV) collagen to the alpha 3(IV), alpha 4(IV) and alpha 5(IV) chains occurs during normal embryogenesis of GBM (ref. 4). Structural abnormalities of type IV collagen have been associated with diverse biological…

medicine.medical_specialtyTranscription GeneticCellular differentiationKidney GlomerulusLIM-Homeodomain ProteinsMolecular Sequence DataBiologyBasement MembraneType IV collagenMiceNail-Patella SyndromeInternal medicineGeneticsmedicineGoodpasture syndromeAnimalsRenal InsufficiencyAlport syndromeNail patella syndromeBasement membraneRegulation of gene expressionHomeodomain ProteinsGlomerular basement membranemedicine.diseaseMolecular biologyMice Mutant StrainsExtracellular Matrixmedicine.anatomical_structureEndocrinologyGene Expression RegulationCollagenTranscription FactorsNature genetics
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