Search results for "Guided tissue regeneration"
showing 10 items of 34 documents
Calcium sulfate and PTFE nonporous barrier for regeneration of experimental bone defects
2008
Made available in DSpace on 2022-04-28T18:55:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-06-01 The aim of the study was to evaluate the possibility to obtain guided bone regeneration with two types of physical barriers (calcium sulfate and PTFE nonporous barrier) in surgical defects created in rat parietal bones. In the right parietal bone the calcium sulfate barrier filled out the whole defect and in the left parietal bone the barrier of PTFE was positioned in the floor and externally to the surgical defect. After 7, 14, 30 and 45 days four animals were sacrificed in each period and the bone containing the defects were submitted to the microscopic analysis. The results of the…
Gene therapy with growth factors for periodontal tissue engineering : a review
2011
The treatment of oral and periodontal diseases and associated anomalies accounts for a significant proportion of the healthcare burden, with the manifestations of these conditions being functionally and psychologically debilitating. A challenge faced by periodontal therapy is the predictable regeneration of periodontal tissues lost as a consequence of disease. Growth factors are critical to the development, maturation, maintenance and repair of oral tissues as they establish an extra-cellular environment that is conducive to cell and tissue growth. Tissue engineering principles aim to exploit these properties in the development of biomimetic materials that can provide an appropriate microen…
Pre‐clinical evaluation of the effect of a volume‐stable collagen matrix on periodontal regeneration in two‐wall intrabony defects
2021
Aim To histologically evaluate the effect of a new collagen matrix on periodontal regeneration. Materials and methods Two-wall intrabony defects were surgically created bilaterally distally to the maxillary first and third pre-molars in beagle dogs. The defects were randomly allocated to open flap debridement either with (test) or without (control) a volume-stable collagen matrix (VCMX). After 12 weeks, the dogs were euthanized, and the specimens histologically processed. Descriptive, histomorphometrical (vertical gain of periodontal tissues) and statistical analyses were then performed. Results Healing was uneventful in most cases. Residual VCMX was still present and showed integration int…
Five-year results following treatment of intrabony defects with enamel matrix proteins and guided tissue regeneration
2004
Treatment with enamel matrix proteins (EMD) or guided tissue regeneration (GTR) has been shown to enhance periodontal regeneration. However, until now there are limited data on the long-term results following these treatment modalities.The aim of the present clinical study was to present the 5-year results following treatment of intrabony defects with EMD, GTR, combination of EMD and GTR, and open flap debridement (OFD).Forty-two patients, each of whom displayed one intrabony defect of a probing depth of at least 6 mm, were randomly treated with one of the four treatment modalities. The following parameters were evaluated prior to surgery, at 1 year and at 5 years after: plaque index, gingi…
Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold.
2021
(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. N…
Biostable Scaffolds of Polyacrylate Polymers Implanted in the Articular Cartilage Induce Hyaline-Like Cartilage Regeneration in Rabbits
2017
[EN] Purpose: To study the influence of scaffold properties on the organization of ¿in vivo¿ cartilage regeneration. Our hypothesis is that stress transmission to the cells seeded inside the scaffold pores or surrounding it, which is highly dependent on the scaffold properties, determine differentiation of both mesenchymal cells and dedifferentiated autologous chondrocytes. Methods: Four series of porous scaffolds made of different polyacrylate polymers, previously seeded with cultured rabbit chondrocytes or without cells preseeded, were implanted in cartilage defects in rabbits. Subchondral bone was always injured during the surgery in order to allow blood to reach the implantation site an…
Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells
2009
For successful bone regeneration tissue engineered bone constructs combining both aspects, namely a high osteogenic potential and a rapid connection to the vascular network are needed. In this study we assessed the formation of pre-vascular structures by human outgrowth endothelial cells (OEC) from progenitors in the peripheral blood and the osteogenic differentiation of primary human osteoblasts (pOB) on micrometric silk fibroin scaffolds. The rational was to gain more insight into the dynamic processes involved in the differentiation and functionality of both cell types depending on culture time in vitro. Vascular tube formation by OEC was assessed quantitatively at one and 4 weeks of cul…
In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures
2013
Recently, porcine acellular dermal matrix (PADM) has been proposed as a possible alternative to autogenous grafts in periodontal plastic surgery. The aim of the present study was to investigate the in vitro responses of four different oral cell lines cultured on a novel PADM. Furthermore, tissue reaction to PADM was evaluated histologically after subcutaneous implantation in mice.Human gingival fibroblasts (HGF), human osteoblast-like cells, human umbilical vein endothelial cells and human oral keratinocytes (HOK) were cultured and transferred on to the PADM. A tissue culture polystyrene surface served as the control. The viability of all tested cell lines on PADM was measured by using the …
Polyurethane and PTFE membranes for guided bone regeneration: histopathological and ultrastructural evaluation.
2010
Made available in DSpace on 2013-08-12T18:17:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-03-01 Made available in DSpace on 2013-09-30T18:35:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:24Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:04:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-03-01 Objective: The purpose of this study was to research a membrane material for use in guided bone regeneration. Study design: In this study, 25 male Wistar rats were used to analyze the biocompatibility and degradation process of biomembranes. The morphol…
Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic.
2011
This study evaluates a new collagen matrix that is designed with a bilayered structure in order to promote guided tissue regeneration and integration within the host tissue. This material induced a mild tissue reaction when assessed in a murine model and was well integrated within the host tissue, persisting in the implantation bed throughout the in vivo study. A more porous layer was rapidly infiltrated by host mesenchymal cells, while a layer designed to be a barrier allowed cell attachment and host tissue integration, but at the same time remained impermeable to invading cells for the first 30 days of the study. The tissue reaction was favorable, and unlike a typical foreign body respons…