Search results for "HAp"

Metal specificity of the Ni(II) and Zn(II) binding sites of the N-terminal and G-domain of E. coli HypB

2021

HypB is one of the chaperones required for proper nickel insertion into [NiFe]-hydrogenase. Escherichia coli HypB has two potential Ni(II) and Zn(II) binding sites—the N-terminal one and the so-called GTPase one. The metal-loaded HypB–SlyD metallochaperone complex activates nickel release from the N-terminal HypB site. In this work, we focus on the metal selectivity of the two HypB metal binding sites and show that (i) the N-terminal region binds Zn(II) and Ni(II) ions with higher affinity than the G-domain and (ii) the lower affinity G domain binds Zn(II) more effectively than Ni(II). In addition, the high affinity N-terminal domain, both in water and membrane mimicking SDS solution, has a…

Latent measles virus infection in Vero cells depending on a temperature-sensitive phenomenon.

1978

A latent infection by measles virus in a line of Vero cells could be maintained only at 37 degrees C. The conditions of temperature nonpermissiveness were associated with some block in virus production and/or release and with the establishment of an autointerference phenomenon. Reduction of the incubation temperature to 33.5 degrees C induced a rather rapid transition from the latent to a lytical infection with a recue of virus. The rescued virus exhibited a restricted capacity to grow at 37 degrees C.

Sexually selected traits evolve positive allometry when some matings occur irrespective of the trait

2014

Positive allometry of secondary sexual traits (whereby larger individuals have disproportionally larger traits than smaller individuals) has been called one of the most pervasive and poorly understood regularities in the study of animal form and function. Its widespread occurrence is in contrast with theoretical predictions that it should evolve only under rather special circumstances. Using a combination of mathematical modeling and simulations, here we show that positive allometry is predicted to evolve under much broader conditions than previously recognized. This result hinges on the assumption that mating success is not necessarily zero for males with the lowest trait values: for examp…

Closure to Experimental Investigation on Compressive Behavior of Bottle-Shaped Struts

2012

Overland flow generation on hillslopes of complex topography: analytical Solutions

2007

The analytical solution of the overland flow equations developed by Agnese et al. (2001; Hydrological Processes15: 3225–3238) for rectangular straight hillslopes was extended to convergent and divergent surfaces and to concave and convex profiles. Towards this aim, the conical convergent and divergent surfaces are approximated by a trapezoidal shape, and the overland flow is assumed to be always one-dimensional. A simple ‘shape factor’ accounting for both planform geometry and profile shape was introduced: for each planform geometry, a brachistochrone profile was obtained by minimizing a functional containing a slope function of the profile. Minima shape factors are associated with brachist…

A multipronged approach to unveil the emerging role of Hsp60 in chronic obstructive pulmonary disease

2011

Inflammation is a major component of chronic obstructive pulmonary disease (COPD) and its cause and mechanisms are still incompletely understood. For example, the role of heat shock proteins (Hsps), many of which are molecular chaperones, has not been explored in detail in COPD, despite the fact that these molecules are known to participate in inflammation in other diseases. It has been shown that extracellular Hsps can signal certain types of T cells, macrophages, dendritic cells, and neutrophils and, thereby, elicit inflammation and immunity. However, these phenomena have not been investigated in COPD despite: a) the increasing awareness of Hsp participation in inflammation and immunity; …

Immunopositivity of heat shock protein 60 as a biomarker of bronchial carcinogenesis

2005

Haptic Visuality and Film Narration. Mapping New Women’s Cinema in Spain

2020

There is a trend in female film production in the 21st century in the context of Spain towards a type of cinema that we could classify as haptic. It is a mode of representation concerned with multisensory expression in film images. In this article, we shall study this haptic visuality in the works of four filmmakers: Isabel Coixet, Paula Ortiz, Mar Coll and Carla Simón, whose films in this century exemplify the trend. The method chosen is textual analysis, which will provide us with the necessary tools to study matters concerning representation (framing), narration (nuclei and catalysis) and communication (spectatorial subject). These three analytical categories together will enable us to s…

Shape Reconstruction of Metallic Objects by Artificial Vision

1997

Quality control in industrial applications has greatly benefited from the development of tools like artificial vision. The artificial vision approach of quality control has the advantage of being able to perform both non-destructive and contactless controls as well as to work mostly in real time. However, in the case of non planar objects to be controlled, dimensional control may become hard to realize. Indeed, due to the bidimensional aspect of the images, a loss of information is unavoidable. Consequently, obtaining depth information from a planar image is not obvious. Most of the existing methods use several cameras1 or several light sources2, this leads to an increase in the processing …

Linguistic and communication education at Polish lessons during the period of cultural change. Musings on the curriculum

2020

The article discusses the challenges posed in the 21st century in terms of linguistic and communication education in terms of teaching Polish as the mother tongue. The author posited that socialisation cannot replace education, which is why one of the major goals of linguistic education in terms of the mother tongue is to satisfy the linguistic and communication needs of pupils resulting from the cultural transformations happening in the modern world. The new tasks which Polish teachers face include the development of the so-called new communicational competence, which enables communication in a world dominated by the media, including digital media, and to counter the process of lowering th…