Search results for "HEPG2"
showing 10 items of 42 documents
Bis(hydroxyphenyl)methane-bisphenol F-metabolism by the HepG2 human hepatoma cell line and cryopreserved human hepatocytes
2011
author cannot archive publisher's version/PDF; International audience; Bisphenol F (BPF) is present in the environment and as a contaminant of food. Humans may, therefore, be exposed to BPF, and an assessment of this risk is required. BPF has been shown to have genotoxic and endocrine-disruptor properties in a human hepatoma cell line (HepG2), which is a model system for studies of xenobiotic toxicity. In this study, we investigated the ability of HepG2 cells to biotransform BPF, because metabolism may affect the observed effects of BPF, and we compared this metabolic capacity with that of human hepatocytes. Cells were incubated for 24 hours with [(3)H]-BPF. The culture medium was then conc…
Saturated and unsaturated free fatty acids (FFA) modulate differently HDL uptake in hepatoma HEPG2 cells
2003
Comparative cytotoxic study of silica materials functionalised with essential oil components in HepG2 cells
2020
[EN] This work evaluated the cytotoxic effect of different EOCs-functionalised silica particle types. The in vitro toxicity of eugenol and vanillin-immobilised SAS, MCM-41 microparticles and MCM-41 nanoparticles was evaluated on HepG2 cells, and compared to free EOCs and pristine materials. The results revealed that free essential oil components and bare silica had a mild cytotoxic effect on HepG2 cells. However, the comparative study showed that free eugenol and vanillin had a milder cytotoxic effect than the equivalent concentrations of immobilised components on the different silica particles, while differences in cell viability between the bare and functionalised particles relied on the …
In silico identification of small molecules as new cdc25 inhibitors through the correlation between chemosensitivity and protein expression pattern
2021
The cell division cycle 25 (Cdc25) protein family plays a crucial role in controlling cell proliferation, making it an excellent target for cancer therapy. In this work, a set of small molecules were identified as Cdc25 modulators by applying a mixed ligand-structure-based approach and taking advantage of the correlation between the chemosensitivity of selected structures and the protein expression pattern of the proposed target. In the first step of the in silico protocol, a set of molecules acting as Cdc25 inhibitors were identified through a new ligand-based protocol and the evaluation of a large database of molecular structures. Subsequently, induced-fit docking (IFD) studies allowed us…
EFFECT OF EXTRACTS FROM LEAVES AND RHIZOMES OF THE SEAGRASS POSIDONIA OCEANICA ON HEPG2 HEPATOCARCINOMA (HCC) CELLS
2022
Ksenobiotiku metabolisma enzīmu citohroma P450 aktivitātes audzēju šūnu līnijās
2017
Jaunu zāļu vielu meklējumi pasaulē notiek ļoti aktīvi. Šobrīd jauno savienojumu īpašību, tai skaitā toksiskuma, izpētei plaši izmanto šūnu līniju modeļus in vitro. Viena no šūnu svarīgākajām sistēmām, kurai ir milzīga ietekme uz jebkura preparāta darbību organismā, ir citohroma P450 sistēma. Tai ir svarīga loma ķīmiskās homeostāzes uzturēšanā organismā gan fozioloģiskos, gan pataloģiskos apstākļos. Citohromi piedalās ļoti daudzu ksenobiotiķu eliminācijā no šūnām. Turklāt, zinot, ka ksenobiotiķu biotransformācijas procesā var veidoties organismam bīstami toksiski metabolīti, zināšanas par citohromu aktivitāti kļūst īpaši svarīgas. Šī darba mērķis ir citohroma P450 sistēmas raksturojums šūnu …
Fluorescent probes on HepG2 cells treated with emergent Fusarium mycotoxins
2012
Dabas vielu lipīdu uzkrāšanās un acetilholīnesterāzes inhibīcija cilvēku aknu šūnu līnijā HepG2 in vitro
2022
Taukaino aknu slimība ir viena no izplatītākajām aknu slimībām attīstītajās valstīs, un tiek iedalīta alkohola un ne-alkohola taukaino aknu slimībā. Taukaino aknu slimībai ir potenciāls progresēt līdz steatohepatītam un cirozei, bet joprojām nav atrastas mērķētas terapijas slimības progresijas palēnināšanai. Maģistra darba mērķis ir pētīt Vaccinium ģints ogu izspaidu ekstraktu iedarbību uz acetilholīna koncentrāciju, acetiholīnesterāzes aktivitāti un lipīdu akumulāciju HepG2 šūnu modelī. Pētījuma rezultāti liecina, ka ogu izspiedu ekstrakti samazina lipīdu akumulāciju šūnās un gan ekstrakti, gan zināmais aknu šūnu funkciju uzlabotājs silimarīns inhibē acetilholīnesterāzes (AChE) aktivitāti.…
Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines.
2009
International audience; Glyphosate-based herbicides are the most widely used across the world; they are commercialized in different formulations. Their residues are frequent pollutants in the environment. In addition, these herbicides are spread on most eaten transgenic plants, modified to tolerate high levels of these compounds in their cells. Up to 400 ppm of their residues are accepted in some feed. We exposed human liver HepG2 cells, a well-known model to study xenobiotic toxicity, to four different formulations and to glyphosate, which is usually tested alone in chronic in vivo regulatory studies. We measured cytotoxicity with three assays (Alamar Blue (R), MTT ToxiLight (R)), plus gen…