Search results for "HLA-B27"

showing 10 items of 26 documents

Inflammasome activation in Ankylosing Spondylitis is associated to gut dysbiosis

2021

Objective: We undertook this study to evaluate the activation and functional relevance of inflammasome pathways in ankylosing spondylitis (AS) patients and rodent models and their relationship to dysbiosis. Methods: An inflammasome pathway was evaluated in the gut and peripheral blood from 40 AS patients using quantitative reverse transcriptase–polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC), flow cytometry, and confocal microscopy, and was compared to that of 20 healthy controls and 10 patients with Crohn’s disease. Bacteria was visualized using silver stain in human samples, and antibiotics were administered to HLA–B27–transgenic rats. The NLRP3 inhibitor MCC950 was admini…

0301 basic medicineMaleInflammasomesmedicine.medical_treatmentInterleukin-1betaInterleukin-23Mice0302 clinical medicineCrohn DiseaseNLRC4Interleukin 23Immunology and AllergyIleitisHLA-B27 AntigenSulfonamidesReverse Transcriptase Polymerase Chain ReactionCaspase 1Interleukin-17Interleukin-18InflammasomeIleitisMiddle AgedImmunohistochemistryAnti-Bacterial AgentsDNA-Binding ProteinsCytokineIndenesFemaleInterleukin 17Rats Transgenicmedicine.drugAdultAdolescentImmunologyReceptors Cell Surface03 medical and health sciencesAIM2Young AdultRheumatologyIleumNLR Family Pyrin Domain-Containing 3 ProteinmedicineAnimalsHumansSpondylitis AnkylosingFurans030203 arthritis & rheumatologybusiness.industryCalcium-Binding Proteinsmedicine.diseaseGastrointestinal MicrobiomeRatsCARD Signaling Adaptor Proteins030104 developmental biologyCase-Control StudiesImmunologyDysbiosisJointsbusinessDysbiosis
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Brief Report: Functional Interaction of Endoplasmic Reticulum Aminopeptidase 2 and HLA-B27 Activates the Unfolded Protein Response.

2017

Objective: The basic mechanisms underlying the pathogenesis of ankylosing spondylitis (AS) remain unresolved. We previously reported an association of the single-nucleotide polymorphism (SNP) rs2549782 in the endoplasmic reticulum aminopeptidase 2 gene (ERAP2) with AS. It is known that patients homozygous for the G allele (GG) of another ERAP2 SNP, rs2248374, lack expression of ERAP2 (ERAP2 null). The present study utilized this information to study the impact of ERAP2 deficiency on HLA–B27 expression in patients with AS, specifically focusing on the functional interaction of ERAP2 and HLA–B27 in peripheral blood mononuclear cells (PBMCs) from patients with AS and assessing the effects …

0301 basic medicineMaleX-Box Binding Protein 1Aminopeptidases0302 clinical medicineImmunology and AllergyRNA Small InterferingEndoplasmic Reticulum Chaperone BiPHLA-B27 AntigenHeat-Shock ProteinsAlleleBlottingReverse Transcriptase Polymerase Chain ReactionHeat-Shock ProteinSingle NucleotideMiddle AgedFlow CytometryCCAAT-Enhancer-Binding Protein3. Good healthUp-RegulationFemaleWesternHumanAnkylosingAdultAminopeptidaseMononuclearImmunologyBlotting WesternSingle-nucleotide polymorphismBiologyMajor histocompatibility complexSmall InterferingPolymorphism Single NucleotideAdult; Alleles; Aminopeptidases; Blotting Western; CCAAT-Enhancer-Binding Proteins; Cell Line; Female; Flow Cytometry; HLA-B27 Antigen; Heat-Shock Proteins; Humans; Leukocytes Mononuclear; Male; Middle Aged; Polymorphism Single Nucleotide; RNA Small Interfering; Reverse Transcriptase Polymerase Chain Reaction; Spondylitis Ankylosing; Unfolded Protein Response; Up-Regulation; X-Box Binding Protein 1; Immunology and Allergy; Rheumatology; ImmunologyCell Line03 medical and health sciencesDownregulation and upregulationRheumatologyHumansSpondylitis AnkylosingAllelePolymorphismAlleles030203 arthritis & rheumatologySpondylitiHLA-B27LeukocyteEndoplasmic reticulum aminopeptidase 2X-Box Binding Protein 1Molecular biologySettore MED/16 - Reumatologia030104 developmental biologyUnfolded protein responsebiology.proteinCCAAT-Enhancer-Binding ProteinsLeukocytes MononuclearUnfolded Protein ResponseRNAArthritisrheumatology (Hoboken, N.J.)
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No diagnostic utility of antibody patterns against Klebsiella pneumoniae capsular serotypes in patients with axial spondyloarthritis vs. patients wit…

2017

OBJECTIVES: To investigate whether antibody response patterns against Klebsiella pneumoniae capsular serotypes can discriminate patients with axial spondyloarthritis (axSpA) from patients with non-specific low back pain (LBP).METHOD: Immunoglobulin (Ig)G and IgA antibodies against K. pneumoniae capsular serotypes K2, K26, K36, and K50 were measured, and antibody seropositivity compared between groups and analysed for patient correlation in five different groups: (a) 96 patients fulfilling the Assessment of SpondyloArthritis International Society (ASAS) classification criteria for axSpA; (b) 38 patients with either a positive magnetic resonance imaging (MRI) scan as defined by ASAS or a posi…

0301 basic medicineSerotypeMaleCross-sectional studyKlebsiella pneumoniaeDenmarkGastroenterology0302 clinical medicineImmunology and AllergyHLA-B27 Antigenbiologymedicine.diagnostic_testGeneral MedicineLow back painAntibodies BacterialMagnetic Resonance ImagingKlebsiella pneumoniaeC-Reactive ProteinFemaleAntibodymedicine.symptomAdultmedicine.medical_specialtyAdolescentImmunologyHuman leukocyte antigenSerogroup03 medical and health sciencesYoung AdultRheumatologyInternal medicineJournal ArticlemedicineHumansSpondylitis AnkylosingLow back painSacroiliitisBacterial Capsules030203 arthritis & rheumatologyAnkylosing spondylitisbusiness.industryta1182Magnetic resonance imagingaxial spondyloarthritismedicine.diseasebiology.organism_classificationantibody response patternsImmunoglobulin A030104 developmental biologyCase-Control StudiesImmunoglobulin GImmunologybiology.proteinSpondylarthropathiesbusinessLow Back Pain
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CD4+ and CD8+ clonal T cell expansions indicate a role of antigens in ankylosing spondylitis; a study in HLA-B27+ monozygotic twins.

2001

SUMMARYAnkylosing spondylitis (AS) is a complex genetic disease in which both MHC and non-MHC genes determine disease susceptibility. To determine whether the T cell repertoires of individuals with AS show signs of increased stimulation by exogenous antigens, CD4+ and CD8+ T cell subsets of five monozygotic HLA-B27+ twins (two concordant and three discordant for AS) and CD8+ T cell repertoires of three healthy HLA-B27+ individuals were characterized by TCR β-chain (TCRB) CDR3 size spectratyping. Selected TCRB-CDR3 spectra were further analysed by BJ-segment analysis and TCRB-CDR3 from expanded T cell clones were sequenced. In an analysis of all data (519/598 possible TCRB-CDR3 spectra), AS …

AdultCD4-Positive T-LymphocytesT cellImmunologyNeuroimmunologyReceptors Antigen T-CellTwinsMonozygotic twinchemical and pharmacologic phenomenaBiologyCD8-Positive T-LymphocytesMajor histocompatibility complexAntigenmedicineImmunology and AllergyHumansSpondylitis AnkylosingHLA-B27 AntigenAgedHLA-B27T-cell receptorCell DifferentiationT lymphocyteMiddle Agedmedicine.anatomical_structureImmunologybiology.proteinCD8Clinical and experimental immunology
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Elevated levels of anti-endotoxin antibodies in patients with bilateral idiopathic acute anterior uveitis

2010

. Purpose:  Endotoxins have been proved to be responsible for acute anterior uveitis (AAU) in animals in a well-established experimental model of endotoxin-induced uveitis (EIU). The purpose of our study was the detection of antibodies against endotoxins of selected enterobacteria in the serum of patients with idiopathic AAU and searching for correlations between the levels of these antibodies and the presence of HLA-B27 antigen as well as characteristic signs of EIU such as bilaterality and the absence of spontaneous recurrences of the disease. Methods:  Reactions of serum IgG antibodies with lipopolysaccharides (LPSs) of Escherichia coli O1, E. coli O10, E. coli O111, E. coli J5, and Kleb…

AdultLipopolysaccharidesMaleKlebsiella pneumoniaeEnzyme-Linked Immunosorbent AssayInflammationDiseaseEye Infections BacterialAntigenGram-Negative BacteriaEscherichia coliHumansMedicineHLA-B27 Antigenbiologybusiness.industryIncidence (epidemiology)General MedicineMiddle Agedmedicine.diseasebiology.organism_classificationAntibodies BacterialUveitis AnteriorKlebsiella pneumoniaeOphthalmologyImmunoglobulin GAcute DiseaseImmunologybiology.proteinEtiologyFemaleAntibodymedicine.symptomGram-Negative Bacterial InfectionsbusinessUveitisActa Ophthalmologica
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Disease-associated polymorphisms in ERAP1 do not alter endoplasmic reticulum stress in patients with ankylosing spondylitis

2014

The mechanism by which human leukocyte antigen B27 (HLA-B27) contributes to ankylosing spondylitis (AS) remains unclear. Genetic studies demonstrate that association with and interaction between polymorphisms of endoplasmic reticulum aminopeptidase 1 (ERAP1) and HLA-B27 influence the risk of AS. It has been hypothesised that ERAP1-mediated HLA-B27 misfolding increases endoplasmic reticulum (ER) stress, driving an interleukin (IL) 23-dependent, pro-inflammatory immune response. We tested the hypothesis that AS-risk ERAP1 variants increase ER-stress and concomitant pro-inflammatory cytokine production in HLA-B27(+) but not HLA-B27(-) AS patients or controls. Forty-nine AS cases and 22 healthy…

AdultMaleAnkylosing Spondylitismedicine.medical_treatmentImmunologyInflammationSingle-nucleotide polymorphismDiseaseBiologyERAP1AminopeptidasesPolymorphism Single NucleotideMinor Histocompatibility AntigensYoung AdultGene expressionGeneticsmedicineHumansSpondylitis AnkylosingERAP1 Ankylosing SpondylitisEndoplasmic Reticulum Chaperone BiPSpondylitisHLA-B27 AntigenGenetics (clinical)InflammationAnkylosing spondylitisEndoplasmic reticulumMiddle AgedEndoplasmic Reticulum Stressmedicine.diseaseCytokineImmunologyFemalemedicine.symptomGenes & Immunity
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klebsiella pneumoniae-reactive t cells in blood and synovial fluid of patients with ankylosing spondylitis comparison with hla–b27 + healthy control …

1995

Objective To study the frequency of Klebsiella pneumoniae-responsive T cells in the peripheral blood (PB) of ankylosing spondylitis (AS) patients compared with that in healthy HLA-B27+ donors, and to examine T lymphocyte clones (TLC) derived from AS patient synovial fluid (SF) for the presence of Klebsiella reactivity. Methods Limiting dilution analysis of PB T cells in 8 patients with active AS and in 8 HLA-B27+ healthy subjects was used to determine the frequency of PB T cells responsive to K pneumoniae and Escherichia coli GroEL. SF T cells from a patient with active AS were cloned, and 125 TLC were characterized in proliferation assays. Results There were fewer T cells in the PB of AS p…

AdultMaleCellular immunityT-LymphocytesT cellImmunologyEpitopeEpitopesImmune systemRheumatologyReference ValuesSynovial FluidMethodsmedicineHumansImmunology and AllergySynovial fluidSpondylitis AnkylosingPharmacology (medical)HLA-B27 AntigenAgedHLA-B27Blood Cellsbusiness.industryChaperonin 60T lymphocyteMiddle AgedMolecular biologyClone CellsKlebsiella pneumoniaePhenotypemedicine.anatomical_structureImmunologyFemaleBacterial antigenbusinessArthritis & Rheumatism
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Evidence that autophagy, but not the unfolded protein response, regulates the expression of IL-23 in the gut of patients with ankylosing spondylitis …

2013

OBJECTIVES: Interleukin (IL)-23 has been implicated in the pathogenesis of ankylosing spondylitis (AS). The aim of the study was to clarify the mechanisms underlying the increased IL-23 expression in the gut of AS patients. METHODS: Consecutive gut biopsies from 30 HLA-B27(+) AS patients, 15 Crohn's disease (CD) patients and 10 normal subjects were obtained. Evidence for HLA-B27 misfolding was studied. Unfolded protein response (UPR) and autophagy were assessed by RT-PCR and immunohistochemistry. The contribution of UPR and autophagy in the regulation of IL-23 expression was evaluated in in vitro experiments on isolated lamina propria mononuclear cells (LPMCs). RESULTS: Intracellular coloca…

AdultMaleProtein FoldingBiopsyImmunologyATG5Gene ExpressionInflammationdigestive systemArticleGeneral Biochemistry Genetics and Molecular BiologyATG12Young AdultCrohn DiseaseRheumatologyDownregulation and upregulationSettore BIO/13 - Biologia Applicataankylosing spondylitisAutophagymedicineInterleukin 23HumansImmunology and AllergySpondylitis AnkylosingHLA-B27 AntigenAgedMucous Membranebusiness.industryAutophagyInterleukinIleitisMiddle AgedIntestinesInterleukin 23Settore MED/16 - ReumatologiaImmunologyInterleukin-23 Subunit p19Unfolded Protein ResponseUnfolded protein responseFemalemedicine.symptombusinessAnnals of the Rheumatic Diseases
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HLA-B27-derived peptides as autoantigens for T lymphocytes in ankylosing spondylitis

1997

Objective.To study whether peptides derived from the HLA-B27 molecule sequence can stimulate peripheral blood T lymphocytes (PBL) from patients with HLA-B27-associated spondylarthropathies. Methods.PBL from 55 HLA-B27+ patients with ankylosing spondylitis (AS), 28 HLA-B27+ patients with other spondylarthropathies, 7 rheumatoid arthritis patients, and 30 HLA-B27+ and 22 HLA-B27- healthy controls were tested in lymphocyte proliferation assays with 4 synthetic peptides derived from the HLA-B*2705 molecule. Results. A 13-mer peptide (B27PA) induced significant proliferative responses in 17 of the 55 AS patients (stimulation index [SI] 2.5–17.5), as well as in 3 of the HLA-B27+ healthy controls …

AdultMalemusculoskeletal diseasesAdolescentT-LymphocytesT cellImmunologyEnzyme-Linked Immunosorbent AssayLymphocyte proliferationLymphocyte ActivationMajor histocompatibility complexAutoantigensCell LineInterferon-gammaRheumatologyAntigenHumansImmunology and AllergyMedicineSpondylitis AnkylosingPharmacology (medical)SpondylarthropathiesHLA-B27 AntigenAgedHLA-B27biologybusiness.industryT-cell receptorReceptors Antigen T-Cell gamma-deltaT lymphocyteMiddle AgedFlow CytometryPhenotypemedicine.anatomical_structureImmunologybiology.proteinFemalePeptidesbusinessArthritis & Rheumatism
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No primary association between LMP2 polymorphisms and extraspinal manifestations in spondyloarthropathies

1997

OBJECTIVE—To investigate the potential role of the HLA-linked LMP2 (low molecular weight protein) gene polymorphisms in conjunction with DR4 and DR7 on extraspinal disease manifestations in HLA-B27 positive patients with spondyloarthropathy.
METHODS—172 patients with spondyloarthropathy, 46 healthy, HLA-B27 positive blood donors, and 99 unrelated controls were typed for HLA-class I and II antigens. LMP2 alleles were determined by polymerase chain reaction and subsequent restriction enzyme digestion.
RESULTS—There were statistically non-significant increases of DR4 and DR7 in spondyloarthropathy subjects. However these differences did not relate to specific extraspinal manifestations. There …

AdultMalemusculoskeletal diseasesLinkage disequilibriumAdolescentSpondyloarthropathyImmunologyHLA-DR7 AntigenDiseaseGeneral Biochemistry Genetics and Molecular BiologyGenetic determinismUveitisPathogenesisRheumatologyCorrespondenceGenotypeHLA-DR4 Antigenotorhinolaryngologic diseasesmedicineHumansImmunology and AllergyAlleleskin and connective tissue diseasesHLA-B27 AntigenConcise ReportsAgedAged 80 and overPolymorphism Geneticbusiness.industryArthritisProteinsMiddle Agedmedicine.diseaseGenotype frequencyCysteine Endopeptidasesstomatognathic diseasesImmunologyFemaleSpinal DiseasesbusinessAnnals of the Rheumatic Diseases
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