Search results for "HLA"

Quantum dots functionalised artificial peptides bioinspired to the D1 protein from the Photosystem II of Chlamydomonas reinhardtii for endocrine disr…

2021

Herein we describe the design and synthesis of novel artificial peptides mimicking the plastoquinone binding niche of the D1 protein from the green photosynthetic alga Chlamydomonas reinhardtii, also able to bind herbicides. In particular, molecular dynamics (MD) simulations were performed to model in silico the behaviour of three peptides, D1Pep70-H, D1Pep70-S264K and D1Pep70-S268C, as genetic variants with different affinity towards the photosynthetic herbicide atrazine. Then the photosynthetic peptides were functionalised with quantum dots for the development of a hybrid optosensor for the detection of atrazine, one of the most employed herbicides for weed control in agriculture as well …

Zabytkowe organy w kościele parafialnym pw. św. Antoniego w Pieszycach

2013

Na terenie diecezji świdnickiej znajduje się wiele cennych i zabytkowych organów o szczególnej wartości historycznej i brzmieniowej. Z powodzeniem wykorzystywane mogą być nie tylko w liturgii, ale także jako instrumenty koncertowe. Do tej grupy należą niewątpliwie organy w kościele parafialnym pw. św. Antoniego w Pieszycach. Zostały wybudowane w 1874 roku przez firmę Schlag & Söhne ze Świdnicy. Posiadały 37 głosów rozłożonych na trzy manuały i pedał. Traktura rejestrów i pierwszego manuału była pneumatyczna, zaś traktura gry pozostałych klawiatur – mechaniczna. Piszczałki ustawiono na wiatrownicach typu stożkowego. W 1915 roku gruntowny remont instrumentu przeprowadził świdnicki zakład Schl…

Byssochlamys nivea inactivation in pineapple juice and nectar using high pressure cycles

2009

The aim of this work was to assess the inactivation of Byssochlamys nivea ascospores in pineapple juice and nectar by combining pressure sequences involving high pressure cycles with relatively mild thermal processing. The effect of 550 and 600 MPa sustained pressures (holding time of 15 min), combinations of sustained pressures and pressure pulses (holding time of 10 s), and pressure cycles (two, three and five cycles of 550 and 600 MPa for 7.5, 5 and 3 min, respectively), at 20, 40, 60, 70, 80 and 90 °C were compared. B. nivea ascospores were inactivated by applying sustained a pressure of 600 MPa at 90 °C for 5 min (juice) and 15 min (nectar), and three and five cycles of pressure at 600…

Pirandello Theater auf deutschen Bühnen

2019

Der Erfolg von Pirandellos Theater in Deutschland und in den deutsch¬sprachigen Ländern (von Ostpreußen bis in die Schweiz) kommt nicht von ungefähr. Es ist hier unmöglich, alle Zusammenhänge zu rekonstruieren, die die deutsche Kultur mit dem Werk und dem Leben Pirandellos verbinden und die von den philologischen und philosophischen Lehrjahren in Bonn bis hin zu seinem Tode reichen. Die deutsche Kultur und mehr noch Deutschland haben jedenfalls einen besonderen Stellenwert in seinem Werk und in seinem Leben. Es war in Deutschland am Ende des neunzehnten Jahrhunderts, als er die ersten Schritte als Dichter auf den Spuren von Goethes Römischen Elegien wagte, und es war Deutschland, das ihn al…

Immune-mediated Leukopenia due to Chlamydophila pneumoniae Pneumonia

2015

Ekspansja tradycyjnej muzyki podhalańskiej na północ

2020

Artykuł jest próbą przybliżenia zmian zachodzących w tradycyjnej muzyce Podhala w ciągu ostatnich stu pięćdziesięciu lat oraz jej wpływu na repertuar tradycyjny terenów położonych na północ od tego regionu. Wnioski zostały sformułowane na podstawie źródeł archiwalnych – pisanych i fonograficznych – oraz badań własnych przeprowadzonych w ciągu ostatniej dekady na terenie powiatów myślenickiego, suskiego i nowotarskiego (północna część). Wydaje się, że w XIX wieku istniała daleko idąca wspólnota repertuarowa Podhala i Beskidu, trudno więc prześledzić, czy podobne wątki tekstowe i melodyczne są dowodem na jedność repertuaru, czy może na ścisłą ekspansję muzyki z Podhala w kierunku północnym. W…

Gene polymorphisms and HLA-G expression in spontaneous abortions

2015

Abstract Introduction HLA-G and HLA-E are claimed to play a role in establishing maternal–fetal immune tolerance and in maintaining pregnancy. The presence of polymorphism in the HLA-G gene could cause a deficient or excessive expression of the HLA-G and HLA-E molecules. These anomalies could eventually cause pregnancy losses. Materials and methods Clinical study. A total of 90 patients were included in this study. These patients suffered spontaneous abortions between weeks 6 and 11 of pregnancy. We have analysed the most important polymorphisms of the HLA-G gene through different genetic studies and HLA-G and HLA-E expression through immunostaining in human cytotrophoblast cells from first…

HUMAN LEUKOCYTE ANTIGEN POLYMORPHISMS IN ITALIAN PRIMARY BILIARY CIRRHOSIS: A MULTICENTER STUDY OF 664 PATIENTS AND 1992 HEALTHY CONTROLS

2008

Genetic factors are critical in determining susceptibility to primary biliary cirrhosis (PBC), but there has not been a clear association with human leukocyte antigen (HLA) genes. We performed a multicenter case-control study and analyzed HLA class II DRB1 associations using a large cohort of 664 well-defined cases of PBC and 1992 controls of Italian ancestry. Importantly, healthy controls were rigorously matched not only by age and sex, but also for the geographical origin of the proband four grandparents (Northern, Central, and Southern Italy). After correction for multiple testing, DRB1*08 [odds ratio (OR), 3.3; 95% confidence interval (CI), 2.4-4.5] and DRB1*02 (OR 0.9; 95% CI 0.8-1.2) …

Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry.

2008

TAP is responsible for transferring cytosolic peptides into the ER, where they can be loaded onto MHC molecules. Deletion of TAP results in a drastic reduction of MHC class I surface expression and alters the presented peptide pattern. This key molecule in antigen processing is tackled by several viruses and lost in some tumors, rendering the altered cells less vulnerable to T cell-based immune surveillance. Using the TAP-deficient cell line LCL721.174 and its TAP-expressing progenitor cell line LCL721.45, we identified and quantified more than 160 HLA ligands, 50 of which were presented TAP-independently. Peptides which were predominantly presented on the TAP-deficient LCL721.174 cell line…

Development and characterization of mouse anti-human LMP2, LMP7, TAP1 and TAP2 monoclonal antibodies.

2008

Low molecular mass polypeptides (LMP) 2 and LMP7 and transporter associated with antigen processing (TAP) subunits TAP1 and TAP2 play a crucial role in antigen processing and cell surface expression of HLA class I molecules. Since monoclonal antibodies (mAb) to these molecules will facilitate the analysis of their expression, structure and function in normal and transformed cells, in the present study we have developed these reagents. Specifically anti-LMP2 and LMP7 mAb were generated from BALB/c mice immunized with specific peptides, and anti-TAP1 and TAP2 mAb from BALB/c mice immunized with respective recombinant proteins. mAb VF101-39F7 and VF101-39G5 were shown to be specific for LMP2, …