Search results for "HP"
showing 10 items of 1505 documents
Esquema de derivación consistente de prioridades en un marco de consenso
2019
La toma de decisiones impregna la actividad humana. Con frecuencia, la decisión no es simple porque involucra elementos cualitativos, subjetivos, intangibles. En diversos métodos de decisión multi-criterio, los elementos protagonistas de tales procesos son comparados por pares, y tales comparaciones se utilizan para construir una matriz de comparaciones, de cuyo estudio emerge la decisión. En AHP (Analytic Hierarchy Process), por ejemplo, las prioridades se obtienen vía el vector propio de Perron de tales matrices. Para que la decisión sea adecuada, no obstante, las comparaciones deben ser aceptablemente consistentes. Pero cuando se comparan muchos elementos, la consistencia puede flaquear,…
Metabolic signatures across the full spectrum of non-alcoholic fatty liver disease.
2022
Funder: European Commission
In Vitro Evaluation of Enteric-Coated HPMC Capsules—Effect of Formulation Factors on Product Performance
2020
A comparative study on different enteric-coated hard capsules was performed. The influence of different formulation factors like choice of enteric polymer, triethyl citrate (TEC) concentration (plasticizer), talc concentrations (anti-tacking agent), and different coating process parameters on the sealing performance of the capsule and the disintegration time were investigated. Furthermore, the influence of different disintegration test methods (with disc vs. without disc and 50 mM U.S. Pharmacopoeia (USP) buffer pH 6.8 vs. biopredictive 15 mM phosphate buffer pH 6.5) was evaluated. All formulations showed sufficient but not equivalent acid resistance when tested. Polymer type was the main f…
Genome and phenotype microarray analyses of rhodococcus sp. BCP1 and rhodococcus opacus R7: Genetic determinants and metabolic abilities with environ…
2015
In this paper comparative genome and phenotype microarray analyses of Rhodococcus sp. BCP1 and Rhodococcus opacus R7 were performed. Rhodococcus sp. BCP1 was selected for its ability to grow on short-chain n-alkanes and R. opacus R7 was isolated for its ability to grow on naphthalene and on o-xylene. Results of genome comparison, includ- ing BCP1, R7, along with other Rhodococcus reference strains, showed that at least 30% of the genome of each strain presented unique sequences and only 50% of the predicted proteome was shared. To associate genomic features with metabolic capabilities of BCP1 and R7 strains, hundreds of different growth conditions were tested through Phenotype Microarray, b…
Binding mode analysis of ABCA7 for the prediction of novel Alzheimer's disease therapeutics
2021
Graphical abstract
Performance of a Chromolith RP-18e column for the screening of β-blockers
2009
The chromatographic performance of a monolithic column (Chromolith RP-18e) was comprehensively examined in the isocratic separation of ten beta-blockers, using ACN-water mobile phases, and compared with the performance of three microparticulate RP columns manufactured with different types of silica: Spherisorb ODS-2, Kromasil C18 and XTerra MS C18. The comparison considered the analysis time, selectivity, peak shape (column efficiency and asymmetry) and resolution, and was extended to a wide range of mobile phase compositions. The Chromolith column showed good performance for the analysis of beta-blockers with regard to the packed columns. In terms of selectivity and analysis time, the grea…
Transport policy and climate change: How to decide when experts disagree
2008
Abstract Transport is the sector with the fastest growth of greenhouse gases emissions in many countries. Accumulation of these emissions may cause uncertain and irreversible adverse climate change impacts. In this context, we use the analytic hierarchy process (AHP) to face the question on how to select the best transport policy if the experts have different opinions and beliefs on the occurrence of these impacts. Thus, both the treatment of uncertainty and dissent are examined for the ranking of transport policies. The opinions of experts have been investigated by a means of a survey questionnaire. A sensitivity analysis of the experts’ weights and the criteria’ weights confirms the robus…
An FPGA-Based Adaptive Fuzzy Coprocessor
2005
The architecture of a general purpose fuzzy logic coprocessor and its implementation on an FPGA based System on Chip is described. Thanks to its ability to support a fast dynamic reconfiguration of all its parameters, it is suitable for implementing adaptive fuzzy logic algorithms, or for the execution of different fuzzy algorithms in a time sharing fashion. The high throughput obtained using a pipelined structure and the efficient data organization allows significant increase of the computational capabilities strongly desired in applications with hard real-time constraints.
The reverse transcription signature of N-1-methyladenosine in RNA-Seq is sequence dependent
2015
The combination of Reverse Transcription (RT) and high-throughput sequencing has emerged as a powerful combination to detect modified nucleotides in RNA via analysis of either abortive RT-products or of the incorporation of mismatched dNTPs into cDNA. Here we simultaneously analyze both parameters in detail with respect to the occurrence of N-1-methyladenosine (m1A) in the template RNA. This naturally occurring modification is associated with structural effects, but it is also known as a mediator of antibiotic resistance in ribosomal RNA. In structural probing experiments with dimethylsulfate, m1A is routinely detected by RT-arrest. A specifically developed RNA-Seq protocol was tailored to …
NOseq: amplicon sequencing evaluation method for RNA m6A sites after chemical deamination
2020
Abstract Methods for the detection of m6A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m6A residues in defined amplicons by virtue of their resistance to chemical deamination, effected by nitrous acid. Partial deamination in NOseq affects all exocyclic amino groups present in nucleobases and thus also changes sequence information. The method uses a mapping algorithm specifically adapted to the sequence degeneration caused by deamination events. Thus, m6A sites with partial modification levels of ∼50% were detected in defined amplicons, and this threshold can be lowered to ∼10% by combination with m6A immunoprecipitation. NOseq faithfully d…