Search results for "HPLC"

HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey

2018

The present study reveals the utility of high-performance thin-layer chromatographic (HPTLC) fingerprinting of phenolic constituents for the authentication of monofloral honeys. The obtained data enables a more complete assessment of honey quality and the identification of emerging threats to honey quality. The developed procedure facilitates differentiation of varietal honeys and detection of honey adulterations. We used an HPTLC fingerprint analysis to determine the characteristic patterns of different honey types (willow, buckwheat, heather, pine honeydew, and manuka honey). The HPTLC chromatograms were used to determine the differences in the botanical origin of the honey samples on the…

Pharmaceutical properties of supramolecular assembly of co-loaded cardanol/triazole-halloysite systems

2015

Halloysite nanotubes were explored as drug carrier for cardanol, which is considered as a promising natural anticancer active species. To this aim, besides the pristine nanoclay, a chemical modification of the nanocarrier was performed by attaching triazolium salts with different hydrophobicity at the outer surface of the hollow nanotubes. The interaction between cardanol and nanotubes was highlighted in solution by HPLC. This method proved the loading of the drug into the nanotubes. The solid dried complexes formed by pristine and modified halloysite with the cardanol were characterized by IR spectroscopy, thermogravimetric analysis as well as water contact angle to evidence the structure,…

Universal method to determine acidic licit and illicit drugs and personal care products in water by liquid chromatography quadrupole time-of-flight

2016

Graphical abstract

Peptide mapping by reversed-phase high-performance liquid chromatography employing silica rod monoliths.

2003

In this paper, a general procedure is described for the generation of peptide maps of proteins with monolithic silica-based columns. The peptide fragments were obtained by tryptic digestion of various cytochrome c species with purification of the tryptic fragments achieved by reversed-phase high-performance liquid chromatographic methods. Peak assignment of the various peptides was based on evaluation of the biophysical properties of the individual peptides and via mass spectrometric identification. The performance of several different monolithic sorbents prepared as columns of identical cross-sectional dimensions were investigated as part of these peptide mapping studies and the data evalu…

Anti-Bacterial Activity and Online HPLC-DPPH Based Antiradical Kinetics of Medicinal Plant Extracts of High Relevance for Cosmetics Production

2018

The present study provides an assessment of extracts of six medicinal plants for their applicability in cosmetic formulations aimed at anti-UV protection and anti-acne treatment. Ethanol extracts of Chelidonium majus L., Myrtus communis L., Hamamelis virginiana L., Juniperus communis L., Alchemilla vulgaris and Ilex paraguariensis are analysed for the phytochemical content, antimicrobial activity, antiradical activity and antiradical kinetics. The phytochemical composition of the plants is investigated by LC-MS analysis and the most active antioxidants in plant extracts are determined by online HPLC-DPPH assay. The kinetic parameters of the antiradical reaction are determined both for the s…

Comparison of monolithic and microparticulate columns for reversed-phase liquid chromatography of tryptic digests of industrial enzymes in cleaning p…

2011

Abstract Enzymes of several classes used in the formulations of cleaning products were characterized by trypsin digestion followed by HPLC with UV detection. A polymeric monolithic column (ProSwift) was used to optimize the separation of both the intact enzymes and their tryptic digests. This column was adequate for the quality control of raw industrial enzyme concentrates. Then, monolithic and microparticulate columns were compared for peptide analysis. Under optimized conditions, the analysis of tryptic digests of enzymes of different classes commonly used in the formulation of cleaning products was carried out. Number of peaks, peak capacity and global resolution were obtained in order t…

Exploring the apparent properties and Q-markers for quality control of Cornus officinalis

2023

Cornus officinalis Siebold & Zucc. has a high nutritious value and is widely used in food fields. To distinguish the quality of cornus in different areas, this research provided a new strategy for comprehensively evaluating cornus. As a result, 12 iridoid glycosides and 7 polyphenols were identified and quantified by UHPLC-Q-TOF/MS in 13 batches of samples from five different origins. Chromaticity analysis pointed out that pelargonidin-3-O-galactoside, total iridoids, and total polyphenols performed high relation with 1365*. Antioxidant activity and inhibition of α-glucosidase effect correlated with the chemical analysis suggested that iridoid glycosides and polyphenols should represent…

Validation of a multi-residue UHPLC-HRMS method for antibiotics screening in milk, fresh cheese, and whey

2022

Abstract The aim of this study was to develop a UHPLC–HRMS methodology as a suitable tool for the screening of antibiotics in milk, fresh cheese, and whey. Compounds were analysed using an Orbitrap Exactive™ analyser working at a resolving power of 50,000 FWHM in full scan, both in positive and negative electrospray ionization mode, and a high mass accuracy (

Fast one-step liquid chromatography determination of purine compounds in blood with photodiode array detector

2012

COMPARAZIONE DEI PROFILI METABOLICI DI VARIETÀ DI CILIEGIO DOLCE (Prunus avium L.) MEDIANTE TECNICHE DI METABOLOMICA UNTARGETED

La diffusione di cultivar altamente produttive ha influito negativamente sulla tutela dell’agro-biodiversità di Prunus avium L. determinando disinteresse nei confronti di varietà meno produttive ma meritevoli di attenzione per il valore delle loro caratteristiche organolettiche e del relativo patrimonio genetico. Il mondo scientifico ha mostrato interesse nei confronti di tale tematica e ha condotto azioni di conservazione in situ ed ex situ per preservare il germoplasma ancora esistente. La presente ricerca analizza l’agrobiodiversità del ciliegio dolce in relazione alla diversa composizione metabolomica dei frutti, espressione del genotipo che le caratterizza. L’attività di ricerca svolta…