Search results for "Handling"
showing 10 items of 345 documents
New ECCO model documents for Material Deposit and Transfer Agreements in compliance with the Nagoya Protocol
2020
The European Culture Collections Organisation presents two new model documents for Material Deposit Agreement (MDA) and Material Transfer Agreement (MTA) designed to enable microbial culture collection leaders to draft appropriate agreement documents for, respectively, deposit and supply of materials from a public collection. These tools provide guidance to collections seeking to draft an MDA and MTA, and are available in open access to be used, modified, and shared. The MDA model consists of a set of core fields typically included in a deposit form to collect relevant information to facilitate assessment of the status of the material under access and benefit sharing (ABS) legislation. It a…
Analytic second derivatives for general coupled-cluster and configuration-interaction models.
2004
Analytic second derivatives of energy for general coupled-cluster (CC) and configuration-interaction (CI) methods have been implemented using string-based many-body algorithms. Wave functions truncated at an arbitrary excitation level are considered. The presented method is applied to the calculation of CC and CI harmonic frequencies and nuclear magnetic resonance chemical shifts up to the full CI level for some selected systems. The present benchmarks underline the importance of higher excitations in high-accuracy calculations.
Molecular Identification of Yeasts Associated with Traditional Egyptian Dairy Products
2009
This study aimed to examine the diversity and ecology of yeasts associated with traditional Egyptian dairy products employing molecular techniques in yeast identification. A total of 120 samples of fresh and stored Domiati cheese, kariesh cheese, and "Matared" cream were collected from local markets and examined. Forty yeast isolates were cultured from these samples and identified using the restriction-fragment length polymorphism (RFLPs) of 5.8S-ITS rDNA region and sequencing of the domains D1 and D2 of the 26S rRNA gene. Yeasts were identified as Issatchenkia orientalis (13 isolates), Candida albicans (4 isolates), Clavispora lusitaniae (Candida lusitaniae) (9 isolates), Kodamaea ohmeri (…
PCR-DGGE fingerprints of microbial succession during a manufacture of traditional water buffalo mozzarella cheese.
2004
D . E R C O L I N I , G . M A U R I E L L O , G . B L A I O T T A , G . M O S C H E T T I A N D S . C O P P O L A . 2003. Aims: To monitor the process and the starter effectiveness recording a series of fingerprints of the microbial diversity occurring at different steps of mozzarella cheese manufacture and to investigate the involvement of the natural starter to the achievement of the final product. Methods and Results: Samples of raw milk, natural whey culture (NWC) used as starter, curd after ripening and final product were collected during a mozzarella cheese manufacture. Total microbial DNA was directly extracted from the dairy samples as well as bulk colonies collected from the plates…
A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally pr…
2007
In this work, a new multiplex single-tube real-time PCR approach is presented for the detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus, three of the more frequent food-borne bacterial pathogens that are usually investigated in a variety of food matrices. The study includes the design and specificity testing, of a new primer and probe specific for Salmonella spp. Reaction conditions were adjusted for the simultaneous amplification and detection of specific fragments in the beta-glucuronidase (uidA, E. coli) and Thermonulease (nuc, Sta. aureus) genes, and in the replication origin sequence (oriC, Salmonella spp.). Melting-curve analysis using a SYBR Green I RTi…
Geo‐referencing naturalistic driving data using a novel method based on vehicle speed
2013
Naturalistic driving is an experimentation model that allows us to recognise the driving modes observing the driver's behaviour at the wheel of a set of people in natural conditions during long periods of observation. This research methodology aims at increasing the representativeness of the data collected in opposition to data stemming from highly controlled laboratory experiments. However, naturalistic driving research designs produce large volumes of data that are difficult to handle. Thus, it is very important to work with suitable methods for representing and interpreting data, allowing us to observe the variability of the results. The aim of this study is to implement a new methodolog…
A framework for data quality handling in enterprise service bus
2013
Enterprise Service Bus (ESB) is proposed to address the application integration problem by facilitating communication among different systems in a loosely coupled, standard-based, and protocol independent manner. Data sources are maintained out of the ESB's control and there should be a mechanism to select the most suitable data source among all available data sources. Especially, when two or more data sources are about the same object. For instance, it is normal to use more than one sensor to measure pressure or temperature at a particular point. Data quality can play an important role in selecting data sources in ESB since quality of data is an essential factor in the success of organizat…
Prediction of arrival times and human resources allocation for container terminal
2011
Increasing competition in the container shipping sector has meant that terminals are having to equip themselves with increasingly accurate analytical and governance tools. A transhipment terminal is an extremely complex system in terms of both organisation and management. Added to the uncertainty surrounding ships’ arrival time in port and the costs resulting from over-underestimation of resources is the large number of constraints and variables involved in port activities. Predicting ships delays in advance means that the relative demand for each shift can be determined with greater accuracy, and the basic resources then allocated to satisfy that demand. To this end, in this article we pro…
Energy performance improvements of a DEC system due to wet heat exchangers
2011
This work concerns an innovative desiccant and evaporative cooling (DEC) system for building air conditioning. The original system is an experimental DEC air handling unit installed at the Solar Laboratory of the University of Palermo and recently modified and updated. With the aim to increase the cooling effect due to water evaporation in the return air flow rate, the sensible heat exchanger was replaced with two cross flow flat plate heat exchangers installed in series, with continuous humidification of the secondary air flow. The new thermodynamic cycle is theoretically described and the realization of the real application is shown. Detailed operational data and energy performance indica…
A new interface to couple thin-layer chromatography with laser desorption/atmospheric pressure chemical ionization mass spectrometry for plate scanni…
2005
An interface to allow on-line qualitative and quantitative full-plate detection and analysis of compounds separated by thin-layer chromatography (TLC) is presented. A continuous wave diode laser is employed as a desorption source. Atmospheric pressure chemical ionization mass spectrometry ionizes and subsequently identifies the desorbed sample molecules. Besides direct laser desorption on untreated TLC plates, graphite particles were used as a matrix to couple in the laser power and improve the efficiency of desorption.