Search results for "Heat"
showing 10 items of 2717 documents
Reverse electrodialysis heat-engine: Case studies of improving energy efficiency through recovery of low temperature excess heat
2018
Reverse Electrodialysis (RED) is a technology for generating electricity from the difference in salinity between two solutions. RED is usually applied to natural water streams with different salinities, like seawater vs. freshwater. In the RED Heat-to-Power project we explore the option of using artificial water solutions operating in a closed loop where the difference in salinity is regenerated in a separation step powered by heat at temperature ranges between 60 and 100°C. We call this system Reverse Electrodialysis Heat Engine (RED HE). In this paper, first we summarise the possible system configurations and the overall amount of excess heat available in Europe for powering the RED HE pr…
A strongly degenerate quasilinear elliptic equation
2005
Abstract We prove existence and uniqueness of entropy solutions for the quasilinear elliptic equation u - div a ( u , Du ) = v , where 0 ⩽ v ∈ L 1 ( R N ) ∩ L ∞ ( R N ) , a ( z , ξ ) = ∇ ξ f ( z , ξ ) , and f is a convex function of ξ with linear growth as ∥ ξ ∥ → ∞ , satisfying other additional assumptions. In particular, this class of equations includes the elliptic problems associated to a relativistic heat equation and a flux limited diffusion equation used in the theory of radiation hydrodynamics, respectively. In a second part of this work, using Crandall–Liggett's iteration scheme, this result will permit us to prove existence and uniqueness of entropy solutions for the corresponding…
Characterization of a nuclear localization signal of canine parvovirus capsid proteins.
1998
We investigated the abilities of synthetic peptides mimicking the potential nuclear localization signal of canine parvovirus (CPV) capsid proteins to translocate a carrier protein to the nucleus following microinjection into the cytoplasm of A72 cells. Possible nuclear localization sequences were chosen for synthesis from CPV capsid protein sequences (VP1, VP2) on the basis of the presence of clustered basic residues, which is a common theme in most of the previously identified targeting peptides. Nuclear targeting activity was found within the N-terminal residues 4-13 (PAKRARRGYK) of the VP1 capsid protein. While replacement of Arg10 with glycine did not affect the activity, replacement of…
Inhibition of stearoyl-CoA desaturase 1 expression induces CHOP-dependent cell death in human cancer cells.
2010
Background Cancer cells present a sustained de novo fatty acid synthesis with an increase of saturated and monounsaturated fatty acid (MUFA) production. This change in fatty acid metabolism is associated with overexpression of stearoyl-CoA desaturase 1 (Scd1), which catalyses the transformation of saturated fatty acids into monounsaturated fatty acids (e.g., oleic acid). Several reports demonstrated that inhibition of Scd1 led to the blocking of proliferation and induction of apoptosis in cancer cells. Nevertheless, mechanisms of cell death activation remain to be better understood. Principal Findings In this study, we demonstrated that Scd1 extinction by siRNA triggered abolition of de nov…
Geldanamycin-induced osteosarcoma cell death is associated with hyperacetylation and loss of mitochondrial pool of heat shock protein 60 (hsp60)
2013
Osteosarcoma is one of the most malignant tumors of childhood and adolescence that is often resistant to standard chemo- and radio-therapy. Geldanamycin and geldanamycin analogs have been recently studied as potential anticancer agents for osteosarcoma treatment. Here, for the first time, we have presented novel anticancer mechanisms of geldanamycin biological activity. Moreover, we demonstrated an association between the effects of geldanamycin on the major heat shock proteins (HSPs) and the overall survival of highly metastatic human osteosarcoma 143B cells. We demonstrated that the treatment of 143B cells with geldanamycin caused a subsequent upregulation of cytoplasmic Hsp90 and Hsp70 w…
Exosomal Heat Shock Proteins as New Players in Tumour Cell-to-cell Communication
2014
Exosomes have recently been proposed as novel elements in the study of intercellular communication in normal and pathological conditions. The biomolecular composition of exosomes reflects the specialized functions of the original cells. Heat shock proteins (Hsps) are a group of chaperone proteins with diverse biological roles. In recent years, many studies have focused on the extracellular roles played by Hsps that appear to be involved in cancer development and immune system stimulation. Hsps localized on the surface of exosomes, secreted by normal and tumour cells, could be key players in intercellular cross-talk, particularly during the course of different diseases, such as cancer. Exoso…
Heat shock protein 10 and signal transduction: a “capsula eburnea” of carcinogenesis?
2006
To date, little is known either about the physical interactions of heat shock protein 10 (Hsp10) with other proteins within the cell or its involvement in signal transduction pathways. Hsp10 has been considered mainly as a partner of Hsp60 in the Hsp60/10 protein folding machine. Only recently, Hsp10 was reported to interact with proteins involved in deoxyribonucleic acid checkpoint inactivation, termination of M-phase, messenger ribonucleic acid export, import of nuclear proteins, nucleocytoplasmic transport, and pheromone signaling pathways. At the same time, Hsp10 expression can be up-regulated in cancer cells, because it accumulates as the cell transformation progresses. Recent data sug…
Predetermined embryonic glial cells form the distinct glial sheaths of the Drosophila peripheral nervous system
2013
International audience; One of the numerous functions of glial cells in Drosophila is the ensheathment of neurons to isolate them from the potassium-rich haemolymph, thereby establishing the blood-brain barrier. Peripheral nerves of flies are surrounded by three distinct glial cell types. Although all embryonic peripheral glia (ePG) have been identified on a single-cell level, their contribution to the three glial sheaths is not known. We used the Flybow system to label and identify each individual ePG in the living embryo and followed them into third instar larva. We demonstrate that all ePG persist until the end of larval development and some even to adulthood. We uncover the origin of al…
Double Face of eHsp70 in Front of Different Situations
2018
The Hsp70 family is one of the best conserved and abundant member of the heat shock proteins (HSP). This family includes several members and in particular one constitutively expressed member (Hsc70) and another one inducibly expressed under several stress conditions (Hsp70). To date, the intracellular functions of Hsp70 are well defined, and increasing evidences establish its roles in the extracellular environment, such as cytoprotection and immunomodulation. Increasing evidences suggest that several cell types are able to release Hsp70 in the extracellular environment, both under physiological and stress conditions. At the same time many release mechanisms have been identified. This chapte…
PTHrP [67-86] regulates the expression of stress proteins in breast cancer cells inducing modifications in urokinase-plasminogen activator and MMP-1 …
2003
It was previously reported that a midregion domain of parathyroid hormone-related protein (PTHrP), that is, [67-86]-amide, is able to restrain growth and promote matrigel penetration by the 8701-BC cell line, derived from a biopsy fragment of a primary ductal infiltrating carcinoma of the human breast, and that cell invasion in vitro is drastically impaired by inactivation of urokinase-plasminogen activator (uPa). In this study we started a more detailed investigation of the possible effects on gene expression arising from the interaction between PTHrP [67-86]-amide and 8701-BC breast cancer cells by a combination of conventional-, differential display-and semi-quantitative multiplex-polyme…