Search results for "Hemin"
showing 10 items of 93 documents
A FRET-based probe for fluorescence sensing of sulfide/sulfite analytes, using a novel long-wavelength water-soluble 7-hydroxycoumarin as report…
2015
International audience; A FRET-based fluorescent probe for the detection of sulfide and/or sulfite in aqueous buffer, was constructed by connecting the quencher moiety nitrobenzofurazan (7-nitro-1,2,3-benzoxadiazole, NBD) to a water-soluble 3-(2-benzimidazolyl)-7-hydroxycoumarin carboxylic acid through a piperazine linker. This probe exhibits good selectivity and high sensitivity for sulfite and sulfide over cysteine and other potential analytes. Furthermore, this is one of the few examples of fluorogenic coumarins whose solubility in water is maintained upon its conversion into reaction-based fluorescent probes.
Novel scaffold of natural compound eliciting sweet taste revealed by machine learning
2020
Abstract Sugar replacement is still an active issue in the food industry. The use of structure-taste relationships remains one of the most rational strategy to expand the chemical space associated to sweet taste. A new machine learning model has been setup based on an update of the SweetenersDB and on open-source molecular features. It has been implemented on a freely accessible webserver. Cellular functional assays show that the sweet taste receptor is activated in vitro by a new scaffold of natural compounds identified by the in silico protocol. The newly identified sweetener belongs to the lignan chemical family and opens a new chemical space to explore.
Quels apports des modèles de management stratégique à l'analyse de la compétitivité du chemin de fer ?
2018
National audience; La SNCF polarise régulièrement l'actualité économique, sociale et politique. Elle a connu en 2018 une nouvelle réforme qui l'a plongée dans plusieurs mois de crise. Plus largement, c'est l'ensemble des chemins de fer européens qui ont fait l'objet de réformes. Associant universitaires, consultants et syndicalistes, ce livre fait le point sur les évolutions récentes de ce mode de transport dans différents pays : Allemagne, Espagne, France, Italie, Royaume-Uni, Suisse. Il s'intéresse aux modalités d'ouverture à la concurrence et à leurs résultats, à l'économie et aux performances des compagnies, au devenir et aux enjeux du fret, aux statuts des personnels, au management de …
Syndicalistes cheminots et image de la Résistance
2001
Recommandation de parcours de formation dans un contexte mobile
2013
National audience; Les récentes avancées dans les technologies de l'information et de la communication ont vu naître de nouvelles formes d'enseignement. L'apprentissage à distance classique s'enrichit et se transforme pour donner jour à un apprentissage plus flexible, accessible sur de multiples supports, à toute heure et en tout lieu : l'apprentissage mobile. Nos travaux portent sur la conception d'un système de recommandation basé sur le contenu, modélisé en utilisant les technologies du web sémantique. La recommandation prendra en compte l'objectif de formation, mais également les supports disponibles pour dispenser cette formation, les préférences personnelles de l'apprenant, ou encore …
Engineering faster transglycosidases and their acceptor specificity
2019
Transglycosidases are enzymes that have the potential to catalyze the synthesis of a wide range of high-value compounds starting from biomass-derived feedstocks. Improving their activity and broadening the substrate range are important goals to enable the widespread application of this family of biocatalysts. In this work, we engineered 20 mutants of the rice transglycosidase Os9BGlu31 and evaluated their catalysis in 462 reactions over 18 different substrates. This allowed us to identify mutants that expanded their substrate range and showed high activity, including W243L and W243N. We also developed double mutants that show very high activity on certain substrates and exceptional specific…
Synthesis and antibacterial activities of cadiolides A, B and C and analogues
2015
International audience; The one-pot multicomponent synthesis of natural butenolides named cadiolides A, B, C and analogues has been realized. The antibacterial structure activity relationship shows that the presence of phenolic hydroxyl groups and the number and position of bromine atoms on the different aromatic rings are important features for antibacterial activity, besides it was demonstrated the tolerance of both benzene and furan ring at position 3 of the butenolide nucleus. Furthermore, none of the most relevant antibacterial compounds showed any cytotoxicity in freshly isolated human neutrophils.
Template-Assembled Synthetic G-Quartets (TASQ) hydrosolubles : du ligand de quadruplexes d'ADN et d'ARN à la plateforme catalytique
2013
Natural G-quartets, a cyclic and coplanar array of four guanine residues held together via Hoogsteen H-bond network, have recently received much attention due to their involvement in G-quadruplex-DNA, an alternative higher-order DNA structure strongly suspected to play important roles in key cellular events (chromosomal stability, regulation of gene expression). Besides this, synthetic G-quartets, which artificially mimic native G-quartets, have also been widely studied for their involvement in nanotechnological applications (i.e. nanowires, artificial ion channels, etc.). In contrast, intramolecular synthetic G-quartets, also named template-assembled synthetic G-quartet (TASQ), have been m…
Réforme de la SNCF : en finir avec les données fausses sur les chemins de fer
2018
International audience
An oxidatively damaged G-quadruplex/hemin DNAzyme.
2020
International audience; Oxidative damage of guanine to 8-oxoguanine triggers a partial and variable loss of G-quadruplex/hemin DNAzyme activity and provides clues to the mechanistic origins of DNAzyme deactivation, which originates from an interplay between decreased G-quadruplex stability, lower hemin affinity and a modification of the nature of hemin binding sites.