Search results for "High Resolution"

GFP immunogold staining, from light to electron microscopy, in mammalian cells.

2012

GFP has emerged as an important reporter for monitoring gene expression, protein localization, cell transformation and cell lineage. The development of GFP as a marker in many different biological systems has emphasized the need to image GFP at high resolution. GFP immunogold labeling with colloidal gold particles becomes essential for electron microscopy (EM) ultrastructural detection. Because of the small size, colloidal gold particles require silver enhancement, a procedure to increase the size of the particle as well as gold toning to stabilize the silver layer. GFP preembedding immunogold staining enables high quality cellular-ultrastructural EM analysis mainly for two reasons, on one …

Compared regimes of NDVI and Rainfall in semi-arid regions of Africa

2006

International audience; Bi-monthly normalized difference vegetation index (NDVI) at an 8km spatial resolution from the advanced very high resolution radiometers (AVHRR) was used from 1981 to 1995 to analyse the vegetation response to rainfall supply in semi-arid regions of Africa. Within the 200-600 mm annual rainfall belt, for which the apparent NDVI response to rainfall was the strongest, three regions were selected which exhibited different patterns in their NDVI regimes and/or relationships with rainfall. The regions, located in western, southern and eastern Africa, were split into coherent sub-regions in terms of mean regime of photosynthetic activity through a cluster analysis. Overal…

Surface emissivity retrieval from Digital Airborne Imaging Spectrometer data

2002

[1] A study has been carried out on the most recent algorithms for the estimation of land surface emissivity (e) using high-resolution data (Digital Airborne Imaging Spectrometer, DAIS) over the Rhine Valley (France) and Castilla La Mancha (Spain). Three published methods have been applied for extracting absolute spectral emissivity information from images recorded during the DAISEX experiment in 1999. They are NDVI Thresholds Method (NDVITHM), Normalized Emissivity Method (NEM) and Temperature/Emissivity Separation (TES). These lather two methods were originally designed to work over geological surfaces. Five methods have been used for extracting relative spectral emissivity. They are temp…

Integrated Geophysical Investigations at the Greek Kamarina Site (Southern Sicily, Italy)

2018

Kamarina, located in southern Sicily (Italy), was an important Greek colony since its foundation in the sixth century BC. Archaeological excavations, carried out since the twentieth century, uncovered only limited portions of the site so far. Despite the importance of the Greek colony, the presence of remarkable buildings that archaeologists expected to bring to light has not found fully correspondence in the archaeological excavations. Consequently, the integrated geophysical prospection carried out in the study area is aimed to support and address the future archaeological investigations. After the photographic and thermographic survey obtained by an unmanned aerial vehicle, we performed …

Review of thermal infrared applications and requirements for future high-resolution sensors

2016

High-resolution thermal infrared (TIR) remote sensing has a wide range of applications. In this paper, we describe the different applications and requirements identified in a literature review and during a consultation meeting with researcher experts in different fields. As a result, more than 30 applications were identified within three different fields: 1) land and solid Earth; 2) health and hazards; and 3) security and surveillance. A complete set of requirements (spatial, temporal, and radiometric resolution, algorithms used, and supporting data, among others) for each application is also provided. The results presented in this paper provide useful information to enhance the importance …

High Resolution Time Domain Reflectometry for Dielectric State Monitoring in High Voltage Cables

2017

A high resolution Time Domain Reflectometry system has been designed in order to investigate the possibility of measuring with good accuracy the dielectric properties of high voltage cables insulator materials by means of Time-of-Flight measures. The system employs a dedicated Time-To-Digital Converter in order to achieve a time resolution of 90 ps. By exploiting averaging techniques the resolution has been further increased. Experimental results showed the possibility of measuring the dielectric constant with a resolution of 0.03%.

Seasonal cycles and variability of O<sub>3</sub> and H<sub>2</sub>O in the UT/LMS during SPURT

2005

Abstract. Airborne high resolution in situ measurements of a large set of trace gases including ozone (O3) and total water (H2O) in the upper troposphere and the lowermost stratosphere (UT/LMS) have been performed above Europe within the SPURT project. With its innovative campaign concept, SPURT provides an extensive data coverage of the UT/LMS in each season within the time period between November 2001 and July 2003. Ozone volume mixing ratios in the LMS show a distinct spring maximum and autumn minimum, whereas the O3 seasonal cycle in the UT is shifted by 2 to 3 month later towards the end of the year. The more variable H2O measurements reveal a maximum during spring/summer and a minimum…

The Voyage of Metals in the Universe from Cosmological to Planetary Scales: the need for a Very High-Resolution, High Throughput Soft X-ray Spectrome…

2019

Metals form an essential part of the Universe at all scales. Without metals we would not exist, and the Cosmos would look completely different. Metals are primarily born through nuclear processes in stars. They leave their cradles through winds or explosions, and then start their journey through space. This can lead them in and out of astronomical objects on all scales, ranging from comets, planets, stars, entire galaxies, groups and clusters of galaxies to the largest structures of the Universe. Their wanderings are fundamental in determining how these objects, and the entire universe, evolve. In addition, their bare presence can be used to trace what these structures look like. The scope …

2019

Abstract. The cumulative occurrence of heavy precipitation and flood events during recent years in various Central European locations emphasises the urgent need to improve extreme rainfall observations and forecasts. Precipitation gauges based on a weighing system allow the recording of intense short-term precipitation events with a very high temporal resolution (down to 1 min). In this study, observational data that were collected during the period 2000 to 2016 for 126 stations of two corresponding measuring networks in the Central German state of Hesse were investigated for the first time to answer the following questions: (1) Are the recorded high-resolution precipitation data plausible …

Full-Waveform Terrestrial Laser Scanning for Extracting a High-Resolution 3D Topographic Model: a Case Study on an Area of Archaeological Significance

2014

AbstractThis paper describes a method, which uses full-waveform terrestrial laser scanning to survey the surface of the slope below the Temple of Juno, located in the Valley of the Temples in Agrigento (Sicily, Southern Italy). The surface is characterized by the presence of large rock blocks, which have fallen down from the upper side; possible further detachments of rock blocks would cause a situation of general instability, with a very high risk to the archaeological structures in the near future. The methodology was designed to evaluate the potential of full-waveform laser scanning technology for the production of a very high resolution 3D topographic model of the slope, to be used as a…