Search results for "High Resolution"

Evidence of tephra reworking in loess based on 2D magnetic susceptibility mapping: A case study from Rocourt, Belgium

2016

Abstract In this article, a new and original approach to characterize tephra layers based on high resolution magnetic susceptibility (MS) mapping is applied to the tephra deposits of Rocourt (Belgium). A series of MS maps of selected sections show the 3D morphology of tephra horizons, including local dip and reworked structures of the Rocourt and Eltville tephras, proven by chemical analyses of phenocrysts. At Rocourt, tephras are observed in loess which is an excellent material for testing the efficiency of the magnetic susceptibility mapping of tephra deposits. We also attempted to locate the volcanic source of the Eltville tephra based on the spatial analysis of the thickness of deposits…

Early Diagnosis of Vegetation Health From High-Resolution Hyperspectral and Thermal Imagery: Lessons Learned From Empirical Relationships and Radiati…

2019

[Purpose of Review] We provide a comprehensive review of the empirical and modelling approaches used to quantify the radiation–vegetation interactions related to vegetation temperature, leaf optical properties linked to pigment absorption and chlorophyll fluorescence emission, and of their capability to monitor vegetation health. Part 1 provides an overview of the main physiological indicators (PIs) applied in remote sensing to detect alterations in plant functioning linked to vegetation diseases and decline processes. Part 2 reviews the recent advances in the development of quantitative methods to assess PI through hyperspectral and thermal images.

Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium

2017

AbstractStudies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to…

2020

Abstract The filamentous fungus Neurospora crassa, a model microbial eukaryote, has a life cycle with many features that make it suitable for studying experimental evolution. However, it has lacked a general tool for estimating relative fitness of different strains in competition experiments. To remedy this need, we constructed N. crassa strains that contain a modified csr-1 locus and developed an assay for detecting the proportion of the marked strain using a post PCR high resolution melting assay. DNA extraction from spore samples can be performed on 96-well plates, followed by a PCR step, which allows many samples to be processed with ease. Furthermore, we suggest a Bayesian approach for…

Marked Neurospora crassa strains for competition experiments and Bayesian methods for fitness estimates

2019

AbstractThe filamentous fungusNeurospora crassa, a model microbial eukaryote, has a life cycle with many features that make it suitable for studying experimental evolution. However, it has lacked a general tool for estimating relative fitness of different strains in competition experiments. To remedy this need, we constructedN. crassastrains that contain a modifiedcsr-1locus and developed an assay for detecting the proportion of the marked strain using a post PCR high resolution melting assay. DNA extraction from spore samples can be performed on 96-well plates, followed by a PCR step, which allows many samples to be processed with ease. Furthermore, we suggest a Bayesian approach for estim…

Temporal and spatial patterns of trawl fishing activities in the Adriatic Sea (Central Mediterranean Sea, GSA17)

2020

Abstract Trawl fishing activities have occurred for centuries on large spatial scale in the entire Mediterranean Sea, and today they are considered as one of the main and widespread causes of anthropogenic disturbance and habitat alteration in the marine environment. In order to delineate when, where and how marine ecosystems have been perturbed and to implement ecosystem-based management strategies, the identification and investigation of the spatial and temporal distribution of fishing effort and the fleet dynamics play a key role. In this context, Geospatial Technologies such as the Automatic Identification System (AIS) could represent a useful tool. The aim of the present work is to rec…

High-resolution numerical modelling of the barotropic tides in the Gulf of Gabes, eastern Mediterranean Sea (Tunisia)

2017

International audience; A high-resolution 2D barotropic tidal model was developed for the Gulf of Gabes and used to characterise hydrodynamic processes and tidal dynamics. The model is based on the Regional Ocean Modelling System. It is forced at the open boundaries by the semidiurnal M2 and S2 astronomical components while meteorological forcing has been neglected. The model results show good agreement with observations confirming that it reproduces the gulf's main tidal characteristics reasonably well. In fact, the simulated semidiurnal tidal components M2 and S2 generate important sea level variations and coastal currents. Tidal propagation is directed to the gulf's western sector while …

A Single-sensor High-resolution Panoramic Optical Mapping Configuration for Simultaneous Non-overlapped Complete Atrial and Ventricular Parametric Im…

2017

Nowadays optical mapping (OM) is the primary method for imaging electrophysiologically relevant parameters from the outer surface of Langendorff-perfused hearts. This technique has become essential for comprehensively understanding mechanisms of cardiac propagation during physiological activation, arrhythmia, and therapeutic antiarrhythmic interventions in translational hearts. Panoramic whole heart optical mapping systems, using either multiple cameras, plane mirrors or a combination of both, have been developed to overcome intrinsic visualization limitations to traditional single-sensor designs. However current panoramic OM systems are financially challenging for physiology and engineerin…

2017

Induction of plant resistance against pathogens by defense elicitors constitutes an attractive strategy to reduce the use of fungicides in crop protection. However, all elicitors do not systematically confer protection against pathogens. Elicitor-induced resistance (IR) thus merits to be further characterized in order to understand what makes an elicitor efficient. In this study, the oligosaccharidic defense elicitors H13 and PS3, respectively, ineffective and effective to trigger resistance of grapevine leaves against downy mildew, were used to compare their effect on the global leaf metabolism. Ultra high resolution mass spectrometry (FT-ICR-MS) analysis allowed us to obtain and compare t…

New computational techniques for finite-difference Weighted Essentially Non-Oscillatory schemes and related problems

2014

High-Resolution Shock-Capturing (HRSC) schemes constitute the state of the art for computing accurate numerical approximations to the solution of many hyperbolic systems of conservation laws, especially in computational fluid dynamics. A drawback of these schemes is that most of them use the spectral decomposition of the Jacobian matrix of the system to compute the numerical approximations by local projections to characteristic fields. The numerical solutions obtained are often excellent in terms of resolution, but the computational effort needed may be too high for some problems, especially those for which the spectral information of the flux Jacobian matrix is not available or is quite di…