Search results for "Human fertilization"
showing 10 items of 104 documents
The human first cell cycle: impact on implantation.
2013
The morphology of fertilization events has been related to successful implantation by subjective criteria (pronuclei score, pronuclei symmetry and position). This work first described these events by time-lapse technology and then compared the timings of fertilization events (second polar body extrusion, first and second pronuclei appearance, abuttal and fading) in implanted versus nonimplanted embryos in a 2-year cohort retrospective study. A total of 1448 transferred embryos from 842 patients undergoing intracytoplasmic sperm injection with oocyte donation were monitored, 212 embryos from treatments where the number of gestational sacs matched the number of transferred embryos and 687 emb…
Age-associated telomere shortening in mouse oocytes
2013
Abstract Background Oocytes may undergo two types of aging. The first is induced by exposure to an aged ovarian microenvironment before being ovulated, known as ‘reproductive or maternal aging’, and the second by either a prolonged stay in the oviduct before fertilization or in vitro aging prior to insemination, known as ‘postovulatory aging’. However, the molecular mechanisms underlying these aging processes remain to be elucidated. As telomere shortening in cultured somatic cells triggers replicative senescence, telomere shortening in oocytes during reproductive and postovulatory aging may predict developmental competence. This study aimed to ascertain the mechanisms underlying altered te…
Preliminary studies on reproduction of Puntazzo puntazzo (Gmelin 1789) (Pisces, Sparidae) under controlled conditions
1985
Abstract Experiments were carried out under controlled conditions in September 1983 on the reproduction of Puntazzo puntazzo (sheepshead bream). The techniques adopted to stimulate spawning, the egg characteristics, and the phases of embryonal development until the sixth day after hatching are described. The best spawning results were obtained with intramuscular injections of HCG at 1 I.U./g of body weight. Optimal fertilization and hatching were observed at 22°C, the mean temperature of the sea water during the natural period of reproduction of Puntazzo puntazzo .
Effects of sewage sludge addition to Norway spruce seedlings on nitrogen availability and soil fauna in clear-cut areas
2012
Anaerobically digested and composted sewage sludge (CSS) has been suggested to be a slow-release fertilizer in forestry and an alternative to quick-release inorganic fertilizers. The effects of CSS with or without added carbohydrate on inorganic nitrogen availability and on soil animals were tested in two Norway spruce plantations. Half of the seedlings were individually fertilized with CSS, and the rest were left as controls. Solid sucrose was added to half of the fertilized and untreated seedlings. Soil samples were taken in the autumn in the first and the second year after the treatments. CSS increased soil NH4-N (2100%), the proportion of soil NO3-N, and the N concentration of spruce ne…
Effects of Plastic Mulching and Basal Nitrogen Application Depth on Nitrogen Use Efficiency and Yield in Maize
2018
The demand for increased grain production to support population and consumption growth has led to increased interest in field management approaches that incorporate plastic mulching and fertilization management. The purpose of this study was to investigate the effects of plastic mulching and basal nitrogen (N)-fertilizer application depth on N balance estimations, N use efficiency (NUE) and maize yield. The experiment was conducted in 2014 and 2015 with six treatments: no N fertilizer and no mulching (CK), traditional broadcast N fertilizer with mulching (T0), basal N-fertilizer application at a depth of 6 cm with no mulching (T1), basal N-fertilizer application at a depth of 6 cm with plas…
Differential Responses of Nitrate Reducer Community Size, Structure, and Activity to Tillage Systems
2009
ABSTRACT The main objective of this study was to determine how the size, structure, and activity of the nitrate reducer community were affected by adoption of a conservative tillage system as an alternative to conventional tillage. The experimental field, established in Madagascar in 1991, consists of plots subjected to conventional tillage or direct-seeding mulch-based cropping systems (DM), both amended with three different fertilization regimes. Comparisons of size, structure, and activity of the nitrate reducer community in samples collected from the top layer in 2005 and 2006 revealed that all characteristics of this functional community were affected by the tillage system, with increa…
Effects of tributyltin(IV) chloride on fertilization ofStyela plicata(Ascidiacea: Tunicata): II. Scanning and transmission electron microscopy studies
2003
The morphological aspects of Styela plicata fertilization after treatment with tributyltin(IV) chloride are described by means of scanning and transmission electron microscopy investigations. Alterations have been shown both on female and male gametes; spermatozoa, all the egg envelopes and the mitochondria of the egg cortical cytoplasm are modified in relation to incubation time. As a consequence, the damage to gametes blocks sperm–egg interaction and fertilization does not occur. Copyright © 2003 John Wiley & Sons, Ltd.
An Analysis of the Process of Fertilization and Activation of the Egg
1957
Publisher Summary Fertilization consists fundamentally in the release of a chain of metabolic reactions by virtue of which morphogenesis is started. The result of fertilization is the activation of the egg. This can be achieved also by several agents other than the spermatozoon and it is then spoken of as “artificial activation.” This chapter discusses the structural, physiological, and chemical changes that occur in the egg at fertilization that are significant in the understanding of the mechanisms of the activation. The chapter presents the hypothesis that in the cortex of the unfertilized sea urchin egg an inhibitor–enzyme complex is present. By reacting with some substance introduced b…
Cytochrome oxidase activity in the mitochondria of unfertilized and fertilized sea urchin eggs
1959
Abstract The cytochrome oxidase activity of isolated mitochondria of unfertilized and fertilized eggs of Paracentrotus lividus has been studied. It has been found that whereas in the mitochondria of unfertilized eggs saturation is reached at a cytochrome c concentration of 3.28 × 10−5 M, in those of fertilized eggs this occurs at a concentration of 5.74 × 10−5 M. It is shown that upon fertilization an increase of about 30 per cent of the activity of cytochrome oxidase takes place and no further changes appear to occur until blastula stage.
EFFECTS OF DIFFERENT FERTILIZATION LEVELS ON IBERIS SEMPERFLORENS POT CULTURE
2013
The genus Iberis (family Brassicaceae) includes 30 species of annual and perennial herbs and subshrubs that grow in a wide area from western and central Europe to the Mediterranean Basin. IberissemperflorensL. is a perennial evergreen subshrub (30-60 cm high) endemic to southern Italy and Sicily. Due to its abundance of white flowers and extensive flowering period occurring during the winter months, the species has good potential for pot cultivation and outdoor ornamental use in Mediterranean-type climates. Reliable and efficient propagation methods for I. semperflorens nursery production have been determined. However, there are limited information on cultural techniques of this winter flow…