Search results for "Hybridization"
showing 10 items of 812 documents
Energy and Environmental Assessment of a Hybrid Dish-Stirling Concentrating Solar Power Plant
2022
Although the 2019 global pandemic slowed the growing trend of CO2 concentrations in the atmosphere, it has since resumed its rise, prompting world leaders to accelerate the generation of electricity from renewable sources. The study presented in this paper is focused on the evaluation of the energy and environmental benefits corresponding to the hypothesis of hybridizing a dish-Stirling plant installed on the university campus of Palermo (Italy). These analyses were carried out by means of dynamic simulations based on an accurate energy model validated with the experimental data collected during the measurement campaign that occurred during the period of operation of the reference plant. As…
Presence of endophytic bacteria in Vitis vinifera leaves as detected by Fluorescence in situ hybridization
2010
Fluorescence in situ hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM) was applied to detect and localize bacterial colonies in leaf tissues of Vitis vinifera. Leaves were cleared to minimize the autofluorescence of plant fragments. The use of fluorescently labeled bacterial probe EUB338 on discolored grapevine leaf disks allowed the estimation of the spatial distribution of different bacterial colonies. In particular, bacterial colonies were found in veins, cells, hairs, intercellular spaces, and in cut edges of leaf disks of both non-Acremonium byssoides-colonized and A. byssoides-colonized leaves of five different cultivars. Furthermore, CLSM confirmed th…
ANKRD26-RET - A novel gene fusion involving RET in papillary thyroid carcinoma
2018
Abstract Background Rearrangements of RET are drivers of oncogenesis, traceable in different cancer types as papillary thyroid carcinoma (PTC), non-small cell lung cancer, colorectal or breast cancer. Anchored multiplex PCR based next-generation sequencing (NGS) can detect RET rearrangements involving previously unknown partner genes. Methods A sample of PTC underwent NGS, following detection of RET rearrangement by fluorescence in situ hybridization. Expression analysis of ANKRD26 and RET was performed for the tumor harboring ANKRD26-RET, for corresponding normal thyroid tissue and PTC tumors with representative genetic alterations (BRAFV600E, CCDC6-RET), complemented by a comparative sear…
Origin and evolution of Artemia reproductive and genetic diversity
2017
El modo de reproducción de una especie determina su diversidad genética y, a su vez, su éxito ecológico y evolutivo (Normarck et al., 2003; Simon et al., 2003; De Meeûs et al, 2007). En una población sexual, la recombinación meiótica permite que nuevas combinaciones de genes se formen y destruyan constantemente. De hecho, las poblaciones sexuales son generalmente más diversas genéticamente en comparación con las poblaciones asexuales. Por el contrario, en un linaje estrictamente asexual, donde se supone que la mutación (con la mayoría de mutantes deletéreos) sea la única fuente de diversidad genética, se espera que la diversidad clonal de la población se reduzca en cada generación. Por esto…
Opposing health effects of hybridization for conservation
2021
The continuing decline of many natural plant and animal populations emphasizes the importance of conservation strategies. Hybridization as a management tool has proven successful in introducing gene flow to small, inbred populations, but can be also associated with health risks. For example, hybridization can change susceptibility to infection in either direction due to heterosis (hybrid vigor) and outbreeding depression, but such health effects have rarely been considered in the genetic management of populations. Here, we investigated the effects of experimental outcrossing between the critically endangered Saimaa landlocked salmon (Salmo salar m. sebago) and the genetically more diverse A…
12q21 Interstitial Deletions: Seven New Syndromic Cases Detected by Array-CGH and Review of the Literature.
2022
Interstitial deletions of the long arm of chromosome 12 are rare, with a dozen patients carrying a deletion in 12q21 being reported. Recently a critical region (CR) has been delimited and could be responsible for the more commonly described clinical features, such as developmental delay/intellectual disability, congenital genitourinary and brain malformations. Other, less frequent, clinical signs do not seem to be correlated to the proposed CR. We present seven new patients harboring non-recurrent deletions ranging from 1 to 18.5 Mb differentially scattered across 12q21. Alongside more common clinical signs, some patients have rarer features such as heart defects, hearing loss, hypotonia an…
Chromosome analysis using different staining techniques and fluorescent in situ hybridization in Cerithium vulgatum (Gastropoda: Cerithiidae)
2002
In the present paper one population of the “large” subtidal mollusc Cerithium vulgatum Bruguiere, 1792 (Gastropoda: Cerithiidae) from the Northwestern coast of Sicily was investigated from a karyological point of view. The chromosome complement was Giemsa stained, conventionally karyotyped in 18 homomorphic chromosome pairs (10 bi-armed and 8 mono-armed), and subsequently analysed using silver, CMA3 and DAPI staining, and fluorescent in situ hybridization (FISH) with three repetitive DNA probes [ribosomal DNA (rDNA), (TTAGGG)n and (GATA)n]. FISH with the rDNA probe consistently mapped major ribosomal sites (18S-28S rDNA) in the terminal region of the short arms of one small sized mono-armed…
Nanodevices by DNA based gold nanostructures
2017
In this thesis DNA based structures were utilized to create gold nanostructures for nanosensing and nanoelectronic applications. In the past, both of these fields have been dominated by the conventional lithography methods, e.g., electron beam lithography and UV-lithography, but more recently scaling down the components by these techniques has become increasingly more complex and costly. Especially in the micro- and nanoelectronics, the increase in the component density and thus computational power would require fabrication of sub-10-nm components, which is challenging for the top-down approaches. Aforementioned developments have led researchers to seek alternative methods to fabricate these…
Decorin Expression and Oncosuppression in Human Embryonic Carcinomas
2019
Human embryonic stem cells in culture can transform into malignant, cancer-like cells exhibiting lesser differentiation. After transplantation, these transformed cells can form highly malignant germ cell tumors. In humans, germ cell tumors often appear at gonadal sites, like in the testis. In this study, we examined the expression of small leucine rich proteoglycans in normal and karyotypically abnormal human embryonic stem cells using a publicly available transcriptome data. We also examined the expression of the small leucine rich proteoglycans in healthy human testis and in different human testicular non-seminoma germ cell tumors using IST Online database. Furthermore, we localized the e…
Topography of somatostatin gene expression relative to molecular progenitor domains during ontogeny of the mouse hypothalamus
2010
The hypothalamus comprises alar, basal, and floor plate developmental compartments. Recent molecular data support a rostrocaudal subdivision into rostral (terminal) and caudal (peduncular) halves. In this context, the distribution of neuronal populations expressing somatostatin (Sst) mRNA was analyzed in the developing mouse hypothalamus, comparing with the expression pattern of the genes Orthopedia (Otp), Distal-less 5 (Dlx5), Sonic Hedgehog (Shh), and Nk2 homeobox 1 (Nkx2.1). At embryonic day 10.5 (E10.5), Sst mRNA was first detectable in the anterobasal nucleus, a Nkx2.1-, Shh-, and Otp-positive basal domain. By E13.5, nascent Sst expression was also related to two additional Otp-positiv…