6533b838fe1ef96bd12a51c3
RESEARCH PRODUCT
Presence of endophytic bacteria in Vitis vinifera leaves as detected by Fluorescence in situ hybridization
Valeria FerraroGiancarlo MoschettiSandra Lo PiccoloDanilo ErcoliniAntonio AlfonzoS. BurruanoLuca Settannisubject
confocal laser scanning microscopybiologymedicine.diagnostic_testBurkholderia phytofirmansMicroorganismfungifood and beveragesPlate count agarPlant disease resistancebiology.organism_classificationApplied Microbiology and BiotechnologyEndophyteMicrobiologychemistry.chemical_compoundendophytic bacteriavitis viniferachemistryMicrobial ecologyBotanymedicineSicilyfluorescence in situ hybridizationBacteriaFluorescence in situ hybridizationdescription
Fluorescence in situ hybridization (FISH) in combination with confocal laser scanning microscopy (CLSM) was applied to detect and localize bacterial colonies in leaf tissues of Vitis vinifera. Leaves were cleared to minimize the autofluorescence of plant fragments. The use of fluorescently labeled bacterial probe EUB338 on discolored grapevine leaf disks allowed the estimation of the spatial distribution of different bacterial colonies. In particular, bacterial colonies were found in veins, cells, hairs, intercellular spaces, and in cut edges of leaf disks of both non-Acremonium byssoides-colonized and A. byssoides-colonized leaves of five different cultivars. Furthermore, CLSM confirmed that bacteria were located in different layers of the leaf tissue. At the same time, one cleared disk of each foliar sample was crushed and plated on Plate Count Agar to isolate cultivable endophytic bacteria. Some of these microorganisms were presumptively identified, by partial 16S rRNA gene sequencing, as Burkholderia phytofirmans, thus suggesting their potential role as plant growth promoters and sources of resistance against pathogenic agents, such as fungi.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-02-05 |