Search results for "ICO"

Rac regulation of NtrbohD, the oxidase responsible for the oxidative burst in elicited tobacco cell

2003

Five cDNAs encoding Rac protein homologues to the Rho-related proteins from plants (Rop) were isolated in tobacco, and the function of one of them, Ntrac5, was studied. The Ntrac5 mRNA is repressed when tobacco leaves and cells are treated with the fungal elicitor cryptogein. Tobacco cells were transformed with sense constructs of Ntrac5 or Ntrac5V15, encoding the native GTP/GDP-bound form of this Rac protein homologue or the constitutively active mutant in its GTP-bound form, respectively. Immunological studies indicate that the corresponding protein is continuously located on the plasma membrane (PM). Both types of transformed cells show the same extra-cellular alkalinization as the contr…

Evolutionary history of two allopatric Terricola species (Arvicolinae, Rodentia) from molecular, morphological, and palaeontological data

2008

To investigate the phylogenetic and phylogeographical relationships of arvicolines, we use several Western European ground voles. More particularly, our study is focused on Microtus (Terricola) savii and M. (T.) pyrenaicus. These two allopatric species are usually considered as having originated from the same ancestor, possibly M. (T.) mariaclaudiae. We propose molecular and morphological approaches: nucleotidic data from the mitochondrial cytochrome b and 12S rRNA genes and global morphological analyses from the first lower molar. Four other Terricola species (multiplex, lusitanicus, duodecimcostatus, subterraneus) were added to the data set for both analyses, and two other vole species (C…

Effects of peroxidizing herbicides on protoporphyrin IX levels in non-chlorophyllous soybean cell culture

1990

Abstract The mode of action of 16 peroxidizing herbicides belonging to four different families (diphenyl ethers, oxadiazon, pyridine derivatives, and pyrazole derivatives) has been studied in nonchlorophyllous soybean cell cultures. Whenever possible, we have compared active and inactive compounds. Phytotoxic effects were estimated on the basis of growth inhibition, either in the dark or in the light. Protoporphyrin IX accumulations were estimated in dark-treated samples, using a simple methodology. In all cases, we have found a positive correlation between cellular damages and protoporphyrin IX accumulations. The results provide further evidences in favor of the light-dependent activity of…

Elicitation of tobacco cells with ergosterol activates a signal pathway including mobilization of internal calcium

2003

Abstract Ergosterol interacts with tobacco suspension ( Nicotiana tabacum ) cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Compared with the responses induced by cryptogein, a proteinaceous elicitor from Phytophthora sp., oxidative burst and ΔpH changes were weaker whereas phytoalexin accumulation was higher with ergosterol. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of…

Evaluation of fungicides and sterilants for potential application in the management of Fusarium wilt of banana

2007

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Fusarium wilt is considered one of the most important diseases of bananas. The disease can only be managed by using resistant cultivars and by preventing its introduction into new fields. The objectives of this study were to evaluate newly available fungicides, in vitro and in vivo, for their efficacy against the responsible pathogen Fusarium oxysporum f.sp. cubense, and to evaluate surface sterilants that could be used for sterilization purposes. Of the fung…

Estimation of water vole abundance by using surface indices

1995

A method to estimate the abundance of the fossorial form of the water vole Arvicola terrestris scherman (Shaw, 1801) has been developed, by using surface indicies. Results are compared to the standard method of estimation using trap lines.

Maternal effects on offspring Igs and egg size in relation to natural and experimentally improved food supply

2008

1. Maternal effects have been suggested to function as a mechanism for transgenerational plasticity, in which the environment experienced by the mother is translated into the phenotype of the offspring. In birds and other oviparous vertebrates where early development is within the egg, mothers may be able to improve the viability prospects of their offspring at hatching by priming eggs with immunological and nutritional components. 2. We studied how resource availability affects maternal investment in offspring by feeding Ural owl (Strix uralensis, Pall.) females prior to egg-laying in 3 years of dramatically different natural food conditions. 3. Supplementary feeding prior to laying increa…

2018

Aposematic theory has historically predicted that predators should select for warning signals to converge on a single form, as a result of frequency-dependent learning. However, widespread variation in warning signals is observed across closely related species, populations and, most problematically for evolutionary biologists, among individuals in the same population. Recent research has yielded an increased awareness of this diversity, challenging the paradigm of signal monomorphy in aposematic animals. Here we provide a comprehensive synthesis of these disparate lines of investigation, identifying within them three broad classes of explanation for variation in aposematic warning signals: …

Morphological and molecular variability within the fig cultivar 'Dottato' in the Italian protected designation origin area "fichi di Cosenza"

2017

The morphological and molecular diversity among fig accessions of 'Dottato', found in the PDO "Fichi di Cosenza" area was studied by evaluating 24 morphological traits and by genotyping with 18 microsatellite markers. The microsatellite allelic profiles among the putative clones of 'Dottato' indicated a moderate genetic variability, discriminating unambiguously most of the accessions. Only two groups of identity were found. The average expected and observed heterozygosity were 0.43 and 0.62, respectively. The mean polymorphic information content (PIC) was 0.4, varying from 0.08 (LMFC26) to 0.067 (FCUP 38-6). The morphological clustering allowed the distinction of all genotypes. Some genotyp…

Forage silica and water content control dental surface texture in guinea pigs and provide implications for dietary reconstruction.

2019

Significance Ingesta leave characteristic wear features on the tooth surface, which enable us to reconstruct the diet of extant and fossil vertebrates. However, whether dental wear is caused by internal (phytoliths) or external (mineral dust) silicate abrasives is controversially debated in paleoanthropology and biology. To assess this, we fed guinea pigs plant forages of increasing silica content (lucerne < grass < bamboo) without any external abrasives, both in fresh and dried state. Abrasiveness and enamel surface wear increased with higher forage phytolith content. Additionally, water loss altered plant material properties. Dental wear of fresh grass feeding was similar to lucerne brows…