Search results for "ICSI"
showing 10 items of 27 documents
Valor de los estudios cromosómicos en espermatozoides mediante la técnica de hibridación in situ fluorescente (FISH) en parejas estériles
2013
Los varones estériles presentan una frecuencia elevada de anomalías citogenéticas, algunas de ellas detectables mediante el cariotipo, pero otras limitadas a las células germinales como resultado de meiosis anormales. Con las nuevas técnicas de reproducción asistida como la Inyección Intracitoplásmica de Espermatozoides (ICSI), la fecundación por espermatozoides cromosómicamente anormales puede dar lugar a fallos de implantación, abortos de repetición y a la transmisión de anomalías cromosómicas a la descendencia. La hibridación in situ fluorescente (FISH) de espermatozoides es una técnica de análisis citogenético que, mediante el marcado de los cromosomas con moléculas de ADN fluorescentes…
Importancia del tamaño uterino en el diagnóstico y pronóstico de las mujeres con problemas de fertilidad
2015
1. Título del proyecto: “Importancia del tamaño uterino en el diagnóstico y pronóstico de las mujeres con problemas de fertilidad”. 2. Hipótesis de trabajo: - ¿Es la hipoplasia uterina uno de los factores a valorar en pacientes en estudio de fertilidad? - ¿Cuál es la relación entre el tamaño uterino y un resultado positivo de gestación en un ciclo de una técnica de reproducción asistida? 3. Objetivos: 3.1. Principales: - Valorar el tamaño uterino medido en su diámetro longitudinal definido por la distancia desde orificio cervical externo (OCE) hasta el límite fúndico de la cavidad endometrial: histerometría. Establecer los límites, en centímetros, que se asocien a menores tasas de gestación…
The chemical composition of the aerial parts essential oil of four Phagnalon species collected in Sicily (Italy) and Greece
2022
The genus Phagnalon Cass., included in the Asteraceae family, has a wide distribution, expanding from Macaronesia in the West to the Himalayas in the East, from South France and Nord Italy to Ethiopia and Arabian Peninsula. Various species of Phagnalon have been used in the popular medicine of several countries as medicinal herbs and food. The extracts and the secondary metabolites, have a varied application spectrum at several biological levels, with antimicrobial, antioxidant, antidiabetic, antitumor, etc. properties having been reported. The essential oils of four taxa, Phagnalon rupestre, Phagnalon saxatile var. viride, and Phagnalon rupestre subsp. illyricum var. metlesicsii collected …
Apoptosis in human sperm as a quality marker for a new diagnostic approach of infertile patients
2018
The quality of the sperm DNA is one of the most important molecular markers of male reproductive potential (Bosco, L et al., 2018, Environ Toxicol Pharmacol 58:243-249). The aim of this study was to evaluate sperm DNA fragmentation according to morphology, to predict the probability of selecting a sperm with normal morphology and intact DNA. An observational study was performed on 70 patients with oligoasthenoteratozoospermia. Sperm DNA Fragmentation Index (DFI) and semen parameters such as sperm density, motility and morphology were evaluated in all patients. DFI was calculated using the in situ TUNEL assay. DFI can be determined and thus used as a marker of sperm quality for a diagnostic …
Selection of the best three oocytes for intracytoplasmic sperm injection (ICSI) using apoptotic analysis of cumulus cells
2009
Introduction: We studied the apoptosis rate of the cumulus cells of individual cumulus-oocyte complex (COC), to verify a relationship with clinical outcomes, in terms of pregnancy and implantation rates. Usually oocytes are selected using morphological criteria. We tried to verify if cumulus cell apoptotic rate could be used as a molecular criteria in selecting oocytes with higher implantation potentiality. Material and Methods: The study design consisted in two different trials: in the first we investigated apoptosis rate in cumulus cells of the three selected oocytes, according to Italian law, to be fertilized by intracytoplasmic sperm injection (ICSI). In a second trial, average apoptosi…
On the non-malacostracan crustaceans (Crustacea: Branchiopoda, Copepoda, Ostracoda) from the inland waters of Fthiotida (Greece)
2019
In the frame of the activities of the LIFE11 NAT/GR/1014 ForOpenForests, some water bodies occurring in the "Ethnikos Drymos Oitis" (GR2440004) and "Oros Kallidromo" (GR2440006) (Sterea Ellada) were investigated with the aim of providing a first census of the composition and diversity of their crustacean fauna. Overall, the sampling of 15 water bodies (7 of them listed as "Mediterranean temporary ponds" sensu EU "Habitats Directive") led to the finding of 13 branchiopod, 11 copepod, and 7 ostracod taxa, including 4 species new for mainland Greece, i.e. the copepods Arctodiaptomus alpinus (Imhoff, 1885) and Diaptomus cf. serbicus, and the branchiopods Leptestheria dahalacensis (Rüppel, 1837)…
Correction to: a predictive model for women's assisted fecundity before starting the first IVF/ICSI treatment cycle.
2019
PURPOSE: To introduce a prognostic model for women’s assisted fecundity before starting the first IVF/ICSI treatment cycle. METHODS: In contrast to previous predictive models, we analyze two groups of women at the extremes of prognosis. Specifically, 708 infertile women that had either a live birth (LB) event in the first autologous IVF/ICSI cycle (“high-assisted-fecundity women”, n = 458) or did not succeed in having a LB event after completing three autologous IVF/ICSI cycles (“low-assisted-fecundity women”, n = 250). The initial sample of 708 women was split into two sets in order to develop (n = 531) and internally validate (n = 177) a predictive logistic regression model using a forwar…
The carriers of the A/G-G/G allelic combination of the c.2039 A>G and c.-29 G>A FSH receptor polymorphisms retrieve the highest number of oocytes in …
2016
The objective of this study was the elucidation of the possible role of the single-nucleotide polymorphisms (SNP) at position -29 and 2039 of the FSH receptor gene (FSHR) as independent predictive markers of ovarian response. Indeed, the tailoring of reproductive treatments is crucial for both maximizing the success of IVF patients and obtaining a reduction in hypo- or hyper-response rates. This prospective, observational study analyzed the association of -29 and 2039 FSHR polymorphisms with the number of retrieved oocytes in 140 patients attending an IVF/ICSI cycle for severe male factors (≤5,000,000 spermatozoa/mL) or tubal factors at the ANDROS Day Surgery Clinic, Palermo, Italy. The res…
Effects of work exposure to electromagnetic waves and organic compounds on sperm DNA fragmentation in patients undergoing ICSI
2011
Fertility After Ovarian Cystectomy: How Does Surgery Affect IVF/ICSI Outcomes?
2019
Abstract Introduction For patients considering undergoing assisted reproductive techniques (ART), many concerns arise when persistent ovarian cysts are found. This large study aimed to determine how ovarian cyst removal affects success rates of IVF/ICSI therapies. Methods 550 patients who underwent an IVF/ICSI treatment between 2002 and 2011 with a persistent ovarian cyst ≤ 5 cm before treatment were analyzed retrospectively. 328 patientsʼ preference was to undergo a laparoscopic cystectomy and 222 patients opted for a conservative management. Control subjects included 13 552 patients undergoing IVF/ICSI at the same period of time without an ovarian cyst. Results After adjusting for age, pa…