Search results for "IDENTIFICATION"
showing 10 items of 1600 documents
Identification by PCR of Trichothecene-ProducingFusarium
2008
Phosphate-controlled regulator for the biosynthesis of the dalbavancin precursor A40926
2007
ABSTRACT The actinomycete Nonomuraea sp. strain ATCC 39727 produces the glycopeptide A40926, the precursor of the novel antibiotic dalbavancin. Previous studies have shown that phosphate limitation results in enhanced A40926 production. The A40926 biosynthetic gene ( dbv ) cluster, which consists of 37 genes, encodes two putative regulators, Dbv3 and Dbv4, as well as the response regulator (Dbv6) and the sensor-kinase (Dbv22) of a putative two-component system. Reverse transcription-PCR (RT-PCR) and real-time RT-PCR analysis revealed that the dbv14 - dbv8 and the dbv30 - dbv35 operons, as well as dbv4 , were negatively influenced by phosphate. Dbv4 shows a putative helix-turn-helix DNA-bind…
Identification and modeling of stop activities at the destination from GPS tracking data
2021
Il presente articolo ha lo scopo di analizzare il comportamento turistico a destinazione, con un focus specifico sulle soste effettuate dai turisti nella destinazione. Vengono analizzati dati desunti da dispositivi GPS raccolti su un campione di crocieristi, a partire dai quali e possibile individuare le soste a destinazione `attraverso l’impiego di un opportuno algoritmo. L’effetto delle caratteristiche sociodemografiche e legate all’itinerario intrapreso sul numero di soste effettuate viene studiato attraverso l’impiego di modelli di reggressione di Poisson. I risultati sono di interesse sia da un punto di vista metodologico, legato all’analisi e sintesi di dati GPS, che dal punto di vist…
Direct quantification of cell-free, circulating DNA from unpurified plasma.
2014
Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is known to be accompanied by a reduction of DNA yield. The primer sets were designed to amplify a 90 and 222 bp multi-locus L1PA2 sequence. In the first module, cfDNA concentrations in unpurified plasma were compared to cfDNA concentrations in the eluate and the flow-through of the QIAamp DNA Blood Mini Kit and in the eluate of a phenol-chloroform isoamyl (PCI) based DNA extraction, to elucidate the D…
Talent identification in soccer using a one-class support vector machine
2019
Abstract Identifying potential future elite athletes is important in many sporting events. The successful identification of potential future elite athletes at an early age would help to provide high-quality coaching and training environments in which to optimize their development. However, a large variety of different skills and qualities are needed to succeed in elite sports, making talent identification generally a complex and multifaceted problem. Due to the rarity of elite athletes, datasets are inherently imbalanced, making classical statistical inference difficult. Therefore, we approach talent identification as an anomaly detection problem. We trained a nonlinear one-class support ve…
A Multi-Scale Colour and Keypoint Density-Based Approach for Visual Saliency Detection
2020
In the first seconds of observation of an image, several visual attention processes are involved in the identification of the visual targets that pop-out from the scene to our eyes. Saliency is the quality that makes certain regions of an image stand out from the visual field and grab our attention. Saliency detection models, inspired by visual cortex mechanisms, employ both colour and luminance features. Furthermore, both locations of pixels and presence of objects influence the Visual Attention processes. In this paper, we propose a new saliency method based on the combination of the distribution of interest points in the image with multiscale analysis, a centre bias module and a machine …
Parameter identification for heterogeneous materials by optimal control approach with flux cost functionals
2021
The paper deals with the identification of material parameters characterizing components in heterogeneous geocomposites provided that the interfaces separating different materials are known. We use the optimal control approach with flux type cost functionals. Since solutions to the respective state problems are not regular, in general, the original cost functionals are expressed in terms of integrals over the computational domain using the Green formula. We prove the existence of solutions to the optimal control problem and establish convergence results for appropriately defined discretizations. The rest of the paper is devoted to computational aspects, in particular how to handle high sens…
A framework for assessing frequency domain causality in physiological time series with instantaneous effects.
2013
We present an approach for the quantification of directional relations in multiple time series exhibiting significant zero-lag interactions. To overcome the limitations of the traditional multivariate autoregressive (MVAR) modelling of multiple series, we introduce an extended MVAR (eMVAR) framework allowing either exclusive consideration of time-lagged effects according to the classic notion of Granger causality, or consideration of combined instantaneous and lagged effects according to an extended causality definition. The spectral representation of the eMVAR model is exploited to derive novel frequency domain causality measures that generalize to the case of instantaneous effects the kno…
The Study of Dynamic Objects Identification Algorithms Based on Anisotropic Properties of Generalized Amplitude-Phase Images
2018
The article presents some results of dynamical objects identification technology based on coincidence matrixes of templates and tested objects’ amplitude-phase images (APIm) calculated with discrete Hilbert transforms (DHT). DHT algorithms are modeled on basis of isotropic (HTI), anisotropic (HTA), generalized transforms – AP-analysis (APA) and the difference (residual) relative shifted phase (DRSP-) images to calculate the APIm. The identified objects are recognized as members of classes modeled with 3D templates – images of different types airplanes rotated in space. The dynamic anisotropic properties of APIm causes the increasing of sensitivity to circular angle rotation and make possibl…
An Innovative Structural Dynamic Identification Procedure Combining Time Domain OMA Technique and GA
2022
In this paper an innovative and simple Operational Modal Analysis (OMA) method for structural dynamic identification is proposed. It combines the recently introduced Time Domain–Analytical Signal Method (TD–ASM) with the Genetic Algorithm (GA). Specifically, TD–ASM is firstly employed to estimate a subspace of candidate modal parameters, and then the GA is used to identify the structural parameters minimizing the fitness value returned by an appropriately introduced objective function. Notably, this method can be used to estimate structural parameters even for high damping ratios, and it also allows one to identify the Power Spectral Density (PSD) of the structural excitat…