Search results for "IL-8"

ERK3/MAPK6 controls IL-8 production and chemotaxis

2020

ERK3 is a ubiquitously expressed member of the atypical mitogen activated protein kinases (MAPKs) and the physiological significance of its short half-life remains unclear. By employing gastrointestinal 3D organoids, we detect that ERK3 protein levels steadily decrease during epithelial differentiation. ERK3 is not required for 3D growth of human gastric epithelium. However, ERK3 is stabilized and activated in tumorigenic cells, but deteriorates over time in primary cells in response to lipopolysaccharide (LPS). ERK3 is necessary for production of several cellular factors including interleukin-8 (IL-8), in both, normal and tumorigenic cells. Particularly, ERK3 is critical for AP-1 signaling…

HSP60 activity on human bronchial epithelial cells

2017

HSP60 has been implicated in chronic inflammatory disease pathogenesis, including chronic obstructive pulmonary disease (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, the effects of this stimulation have never been extensively studied. We investigated the effects (pro- or anti-inflammatory) of HSP60 in human bronchial epithelial cells (16-HBE) alone and in comparison with oxidative, inflammatory, or bacterial challenges. 16-HBE cells were cultured for 1–4 h in the absence or presence of HSP60, H2O2, lipopolysaccharide (…

IL-17A induces chromatin remodeling promoting IL-8 release in bronchial epithelial cells: Effect of Tiotropium

2016

Abstract Aims IL-17A plays a key role in the persistence of airway inflammation, oxidative stress, and reduction of steroid-sensitivity in COPD. We studied the effect of IL-17A on chromatin remodeling and IL-8 production. Main methods We measured the levels of IL-8 and IL-17A in induced sputum supernatants (ISS) from healthy controls (HCs), healthy smokers (HSs), and COPD patients by enzyme-linked immunosorbent assay (ELISA). A human bronchial epithelial cell line (16HBE) was stimulated with ISS from HCs, HSs, or COPD subjects. IL-8 was evaluated in 16HBE by Western blot and real-time polymerase chain reaction (PCR). Histone deacetylase 2 (HDAC2), acetyl histone H3 (Ac-His H3) (k9) and inhi…

Variable Release of Interleukin-8 after Hyperosmolar Exposure of Bronchial Epithelial Cell Lines.

2009

Induction of IL-8 production by Helicobacter pylori strains with different cagA genotype and oipA functional status.

2008

Effects of hyperosmolar stimulation on IL-8 release in different bronchial epithelial cell lines

2009

IL-17A induces chromatin remodeling promoting IL-8 and TSLP release in bronchial epithelial cells. Effect of Tiotropium.

IL-1. induces DNA demethylation at genome level and in specific CpG sites of IL-6 and IL-8 genes in human intestinal epithelial cells

2013

Inflammation is a complex physiological response that requires the activity of a sophisticated regulatory network involving the activation of specific genes for defense, tissue repair and remodeling. Although transcriptional activation has been shown to be critical in the regulation of inflammatory genes (1) the role of epigenetic phenomena in the modulation of the inflammatory response is now emerging (2). Specifically, it has been recently reported that proinflammatory stimuli induce DNA demethylation in the interleukin IL-1 promoter of human articular chondrocytes (3). IL-1 cytokine, among several proinflammatory agents, represents an essential player in the inflammatory conditions of …

Induction of IL-8 production by H.pylori strains with different cagA genotype and oipA functional status

2008

Audzēja ekstracelulāro vezikulu loma mezenhimālo cilmes šūnu sekrēcijas profila izmaiņās

2017

Ekstarcelulārās vezikulas ir mazi pūslīši, kas tiek atbrīvoti no šūnām un izlaisti starpšūnu vidē. EV satur saimniekšūnu ģenētisko materiālu vai to daļas un caur tām viena šūna var nodot informācijas signālu otrai šūnai, tādā veidā ietekmējot to darbību. Arī vēža šūnas var atbrīvot šādas vezikulas un iedarboties uz blakusesošajām gan saimniekorganisma šūnām, gan pašām vēža šūnām. Caur šādu komunikāciju vēža šūnas var pārnest savu izmainīto ģenētisko materiālu uz normālajām šūnām un pārregulēt to darbības mehānismus, kas darbotos audzēja šūnu labā. Darba mērķis bija noskaidrot vai audzēja EV var izraisīt IL-8 un VEGF sekrēcijas izmaiņas ādas mezenhimālajās cilmes šūnās. Lai to noskaidrotu, I…