Search results for "IMMUNOASSAY"

showing 6 items of 266 documents

Presence of thyroid hormones in ascidian larvae and their involvement in metamorphosis

2001

In this study we investigated the presence and localization of thyroxine in Ciona intestinalis larvae and its involvement in metamorphosis. To date, the mechanisms regulating the metamorphosis of ascidians remain largely unknown. In vivo treatment of swimming larvae with exogenous L-thyroxine and thiourea, and in vitro experiments utilizing high performance liquid chromatography, radioimmunoassay, and immunoperoxidase staining demonstrate the presence of thyroxine at the larval stage. This suggests that this hormone may participate in the control of metamorphosis and thus play a different role from that observed in adults.

medicine.medical_specialtyanimal structuresmedia_common.quotation_subjectRadioimmunoassayIn vivoInternal medicinemedicineAnimalsCiona intestinalisUrochordataMetamorphosisChromatography High Pressure LiquidSwimmingmedia_commonLarvabiologyfungiMetamorphosis BiologicalRadioimmunoassayGeneral Medicinebiology.organism_classificationIn vitroThyroxineEndocrinologyLarvaThyroid hormonesAnimal Science and ZoologyHormoneJournal of Experimental Zoology
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The Relationship between the Transforming Growth Factor β1 T29C Gene Polymorphism and Left Ventricular Geometry and Function in Hypertensive Subjects

2009

The distribution of the T29C TGFβ1 gene polymorphism was analyzed in 198 hypertensives with left ventricular hypertrophy (LVH) and in 235 hypertensives without LVH. Circulating TGFβ1 levels, procollagen type III levels, microalbuminuria, and left ventricular geometry and function were evaluated in all the hypertensives with LVH subgrouped according to T29C TGFβ1 gene polymorphism. Circulating TGFβ1 was evaluated by ELISA technique, procollagen type III by a specific radioimmunoassay, microalbuminuria by radioimmunoassay, and left ventricular geometry and function by echocardiography. All groups were comparable for gender, age, and sex. Regarding T29C TGFβ1 gene polymorphism, prevalence of T…

medicine.medical_specialtylcsh:Diseases of the circulatory (Cardiovascular) systemSettore MED/09 - Medicina InternaArticle SubjectLeft ventricular hypertrophyExcretionGene PolymorphismInternal medicineGenotypeInternal MedicinemedicineTransforming Growth Factor β1 T29C; Gene Polymorphism; Left Ventricular Geometry; Hypertensioncardiovascular diseasesTransforming Growth Factor β1 T29CEjection fractionbusiness.industryLeft Ventricular GeometryRadioimmunoassaymedicine.diseaseSettore MED/45 - Scienze Infermieristiche Generali Cliniche E PediatricheSettore MED/11 - Malattie Dell'Apparato CardiovascolareEndocrinologylcsh:RC666-701HypertensionMicroalbuminuriaGene polymorphismbusinessResearch ArticleTransforming growth factorInternational Journal of Hypertension
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236 Multicenter evaluation of the abbott AxSYM BNP immunoassay

2004

medicine.medical_specialtymedicine.diagnostic_testbusiness.industryInternal medicineImmunoassaymedicineCardiology and Cardiovascular MedicinebusinessEuropean Journal of Heart Failure Supplements
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Estrogen metabolism modulates bone density in men.

2007

Estrogen is a critical hormone for bone homeostasis in men, but no information is available on the role of estrogen metabolism among men. The aim of this study was to evaluate the effect of estrogen hydroxylation on male bone mineral density (BMD). Participants consisted of 61 healthy Caucasian males (mean age 66.6 +/- 1.0 years). Urinary estrogen metabolites were measured by enzyme-linked immunosorbent assay, serum estradiol by ultrasensitive radioimmunoassay, sex hormone binding globulin by radioimmunoassay, and BMD of the lumbar spine and the proximal femur by dual-energy X-ray absorptiometry. Active estrogen metabolites, 16alpha-hydroxyestrone (16alphaOHE(1)) and estriol (E(3)), positiv…

musculoskeletal diseasesMalemedicine.medical_specialtyBone densitymedicine.drug_classEndocrinology Diabetes and MetabolismMotor ActivityHydroxylationArticleBody Mass IndexEndocrinologySex hormone-binding globulinBone DensityInternal medicinemedicineHumansOrthopedics and Sports MedicineFemoral neckAgedBone mineralAged 80 and overbiologyChemistryEstriolEstriolRadioimmunoassayEstrogensMiddle Agedmusculoskeletal systemEndocrinologymedicine.anatomical_structureCross-Sectional StudiesSteroid 16-alpha-HydroxylaseEstrogenbiology.proteinBody mass indexCalcified tissue international
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The value of synthetic linear epitope analogues of La/SSB for the detection of autoantibodies to La/SSB; specificity, sensitivity and comparison of m…

1998

SUMMARY In a previous study it was shown that La/SSB contains four linear epitopes, p147–154, p291–302, p301–318 and p349–364. The aim of the present study was to investigate the value of the synthetic epitope analogues of the La/SSB autoantigen for the detection of antibodies to La/SSB, in comparison with recombinant La and fragments of this protein. A total of 122 sera with anti-La/SSB activity, from patients with primary Sjögren's syndrome (pSS) or systemic lupus erythematosus (SLE), were tested in various peptide-based assays. In addition, 62 sera from pSS or SLE patients with other autoantibody specificities and 95 sera from healthy individuals were used as controls. The autoantibody s…

systemic lupusantigenic peptidesMolecular Sequence DataImmunologyDot blotPeptideprimary sjogrens-syndromeBiologyAutoantigensSensitivity and SpecificityEpitopelaw.inventionsjogren's syndromeEpitopesAntigenlawmedicineantibodiesHumansImmunology and AllergyAmino Acid SequenceAutoantibodiesImmunoassaychemistry.chemical_classificationla/ssbcriteriaLinear epitopemedicine.diagnostic_testla ss-bAutoantibodyOriginal Articlessystemic lupus-erythematosusautoantigenRecombinant ProteinsSjogren's SyndromeclassificationRibonucleoproteinschemistryImmunoassayImmunologyRecombinant DNAdiagnostic valueb cell epitopesClinical and Experimental Immunology
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Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron

2013

Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator forchlorfenuron (CPPU) is described. The competitive lateral flow immunoassay (LFIA) was based on the immobilization onto a nitrocellulose membrane of an ovalbumin-CPPU conjugate (test line) and on the use of an immunodetection ligand consisting of carbon nanoparticles labeled with an anti-CPPU monoclonal antibody through interaction with a secondary antibody. The presence of CPPU in horticultural…

water samplesCarbon nanoparticlesAnalyteImmunoreactive stripsPyridinesCarbon NanoparticlesBiomedical EngineeringBiophysicsEnzyme-Linked Immunosorbent AssayCPPUBiosensing Techniquesantibody-based immunoassayForchlorfenuronbiosensorHigh-performance liquid chromatographychemistry.chemical_compoundElectrochemistryImmunoassayDetection limitvalidationChromatographyChemistrystrip testPhenylurea Compoundslateral flow immunoassayAntibodies Monoclonalcomputer image-analysisGeneral MedicineCarbonHaptendipstick assayBBP Bioconversionrapid detectionImmunosensingNanoparticlessensitive detectionBiosensorNitrocelluloseBiotechnologyConjugate
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