Search results for "INOS"

Purification, characterization and influence on membrane properties of the plant-specific sphingolipids GIPC

2020

AbstractThe plant plasma membrane (PM) is an essential barrier between the cell and the external environment. The PM is crucial for signal perception and transmission. It consists of an asymmetrical lipid bilayer made up of three different lipid classes: sphingolipids, sterols and phospholipids. The most abundant sphingolipids in the plant PM are the Glycosyl Inositol Phosphoryl Ceramides (GIPCs), representing up to 40% of total sphingolipids, assumed to be almost exclusively in the outer leaflet of the PM. In this study, we investigated the structure of GIPCs and their role in membrane organization. Since GIPCs are not commercially available, we developed a protocol to extract and isolate …

Elicitation of tobacco cells with ergosterol activates a signal pathway including mobilization of internal calcium

2003

Abstract Ergosterol interacts with tobacco suspension ( Nicotiana tabacum ) cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Compared with the responses induced by cryptogein, a proteinaceous elicitor from Phytophthora sp., oxidative burst and ΔpH changes were weaker whereas phytoalexin accumulation was higher with ergosterol. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of…

Metabolite specific effects of solar UV-A and UV-B on alder and birch leaf phenolics

2008

We measured the concentrations of ultraviolet (UV)-absorbing phenolics varying in response to exclusion of either solar UV-B or both solar UV-A and UV-B radiations in leaves of grey alder (Ainus incana) and white birch (Betula pubescens) trees under field conditions. In alder leaves 20 and in birch leaves 13 different phenolic metabolites were identified. The response to UV exclusion varied between and within groups of phenolics in both tree species. The changes in concentration for some metabolites suggest effects of only UV-A or UV-B, which band being effective depending on the metabolite. For some other metabolites, the results indicate that UV-A and UV-B affect concentrations in the sam…

Nest sites of a strong excavator, the Great Spotted Woodpecker Dendrocopos major, in a primeval forest

2017

In managed forests, birds that create their own breeding holes in trees have limited access to substrates in which they can excavate. Therefore, nest site use in these forests possibly reflects availability of substrates more than species preferences. We analysed data on nest sites of Great Spotted Woodpeckers Dendrocopos major collected during 1987–2013 in the strictly protected part of Białowieża National Park in East Poland. The woodpeckers excavated breeding holes in 11 tree species, but species used in individual habitats varied greatly: Alder Alnus glutinosa was almost the only species used in the riverine forest; Common Aspen Populus tremula, Common Hornbeam Carpinus betulus and Pedu…

Nest sites of Middle Spotted Woodpeckers Leiopicus medius in a primeval forest

2016

Forest management usually reduces the diversity and amount of substrates in which woodpeckers can excavate holes. In such conditions the recorded patterns of nest site selection could reflect a more reduced array of substrates than the species' nest site preferences. We analysed new data on nest sites of Middle Spotted Woodpeckers Leiopicus medius collected during 27 breeding seasons (1987–2013) in the strictly protected fragments of the Białowieża National Park in Poland. The birds could use diverse excavation substrates, of various statures, that varied in condition from living and healthy to dead and completely rotten. Middle Spotted Woodpeckers used a wide array of tree species, with li…

2018

BackgroundThe use and partitioning of trophic resources is a central aspect of community function. On the ground of tropical forests, dozens of ant species may be found together and ecological mechanisms should act to allow such coexistence. One hypothesis states that niche specialization is higher in the tropics, compared to temperate regions. However, trophic niches of most species are virtually unknown. Several techniques might be combined to study trophic niche, such as field observations, fatty acid analysis (FAA) and stable isotope analysis (SIA). In this work, we combine these three techniques to unveil partitioning of trophic resources in a tropical and a temperate community. We des…

FOOD MAKES YOU A TARGET: DISENTANGLING GENETIC, PHYSIOLOGICAL, AND BEHAVIORAL EFFECTS DETERMINING SUSCEPTIBILITY TO INFECTION

2010

Genetics, physiology, and behavior are all expected to influence the susceptibility of hosts to parasites. Furthermore, interactions between genetic and other factors are suggested to contribute to the maintenance of genetic polymorphism in resistance when the relative susceptibility of host genotypes is context dependent. We used a maternal sibship design and long- and short-term food deprivation treatments to test the role of family-level genetic variation, body condition, physiological state, and foraging behavior on the susceptibility of Lymnaea stagnalis snails to infection by a trematode parasite that uses chemical cues to locate its hosts. In experimental exposures, we found that sna…

p24 Family Proteins Are Involved in Transport to the Plasma Membrane of GPI-Anchored Proteins in Plants

2020

p24 proteins are a family of type-I membrane proteins that cycle between the endoplasmic reticulum (ER) and the Golgi apparatus via Coat Protein I (COPI)- and COPII-coated vesicles. These proteins have been proposed to function as cargo receptors, but the identity of putative cargos in plants is still elusive. We previously generated an Arabidopsis (Arabidopsis thaliana) quadruple loss-of-function mutant affecting p24 genes from the δ-1 subclass of the p24 delta subfamily (p24δ3δ4δ5δ6 mutant). This mutant also had reduced protein levels of other p24 family proteins and was found to be sensitive to salt stress. Here, we used this mutant to test the possible involvement of p24 proteins in the…

New Hettangian tracks from the Causses Basin (Lozère, southern France) complement the poor fossil record of earliest Jurassic crocodylomorphs in Euro…

2017

International audience; A new vertebrate ichnological assemblage is described from the Hettangian Dolomitic Formation of the Causses Basin, at Le Serre (Lozère, southern France). We report tracks that complement the poor fossil record of lowermost Jurassic crocodylomorphs in Europe. Tetradactyl pes and pentadactyl manus imprints form a unique trackway. Traces are ascribed to Batrachopus isp. and they represent the third occurrence of this ichnogenus in European Hettangian geological formations. This is the first report of Batrachopus in Hettangian deposits of the Causses Basin. The tracks share some similarities with Batrachopus deweyi previously described from the Sinemurian of the Causses…

Four-Year Study on the Bio-Agronomic Response of Biotypes of Capparis spinosa L. on the Island of Linosa (Italy)

2021

The caper plant is widespread in Sicily (Italy) both wild in natural habitats and as specialized crops, showing considerable morphological variation. However, although contributing to a thriving market, innovation in caper cropping is low. The aim of the study was to evaluate agronomic and production behavior of some biotypes of Capparis spinosa L. subsp. rupestris, identified on the Island of Linosa (Italy) for growing purposes. Two years and seven biotypes of the species were tested in a randomized complete block design. The main morphological and production parameters were determined. Phenological stages were also observed. Analysis of variance showed high variability between the biotype…