Search results for "INTRAC"

LA TEMPERATURA INTRACANALARE UTILIZZANDO TRE DIVERSI SISTEMI DI OTTURAZIONE CANALARE A CALDO

2008

Scopo di questo studio è valutare, in vitro, la variazione della temperatura raggiunta all’interno di un canalare radicolare utilizzando tre diverse tecniche di compattazione della guttaperca a caldo. Materiali e Metodi: E’ stato realizzato un modellino sperimentale definito “split-tooth model”. Nella dentina canalare è stato creato un solco e una depressione laterale a 3,5 mm dalla lunghezza di lavoro (WL) all’interno della quale è stata piazzata un termocoppia in cromo-alluminio di tipo K connessa a un termometro digitale e posta a diretto contatto con la guttaperca. Sono state effettutate 60 otturazioni usando coni di guttaperca non standardizzati (Medium), 20 utilizzando il System B, se…

FLUSSO SANGUIGNO CEREBRALE E PRESSIONE INTRACRANICA.

2008

Poligono di Willis e perdite per mescolanza

2008

Large-scale jets from active galactic nuclei as a source of intracluster medium heating: cavities and shocks

2014

The evolution of powerful extragalactic jets is not only interesting by itself, but also for its impact on the evolution of the host galaxy and its surroundings. We have performed long-term axisymmetric numerical simulations of relativistic jets with different powers to study their evolution through an environment with a pressure and density gradient. Our results show key differences in the evolution of jets with different powers in terms of the spatial and temporal scales of energy deposition. According to our results, the observed morphology in X-ray cavities requires that an important fraction of the jet’s energetic budget is in the form of internal energy. Thus, light, lepton-dominated …

An Automatic System for the Analysis and Classification of Human Atrial Fibrillation Patterns from Intracardiac Electrograms

2008

This paper presents an automatic system for the analysis and classification of atrial fibrillation (AF) patterns from bipolar intracardiac signals. The system is made up of: 1) a feature- extraction module that defines and extracts a set of measures potentially useful for characterizing AF types on the basis of their degree of organization; 2) a feature-selection module (based on the Jeffries-Matusita distance and a branch and bound search algorithm) identifying the best subset of features for discriminating different AF types; and 3) a support vector machine technique-based classification module that automatically discriminates the AF types according to the Wells' criteria. The automatic s…

Enzyme-responsive intracellular-controlled release using silica mesoporous nanoparticles capped with ε-poly-L-lysine.

2014

The synthesis and characterization of two new capped silica mesoporous nanoparticles for controlled delivery purposes are described. Capped hybrid systems consist of MCM-41 nanoparticles functionalized on the outer surface with polymer epsilon-poly-L-lysine by two different anchoring strategies. In both cases, nanoparticles were loaded with model dye molecule [Ru(bipy)(3)](2+). An anchoring strategy involved the random formation of urea bonds by the treatment of propyl isocyanate-functionalized MCM-41 nanoparticles with the lysine amino groups located on the epsilon-poly-L-lysine backbone (solid Ru-rLys-S1). The second strategy involved a specific attachment through the carboxyl terminus of…

New method of bone reconstruction designed for skull base surgery

2008

The direct endonasal or transoral transclival approaches to the skull base permit effective, minimally invasive surgery along the clivus. Developing long-term, effective techniques to prevent cerebrospinal fluid (CSF) leaks and their consequences (infection and delayed healing) remains a major challenge. In this study we describe a method of bone reconstruction newly developed by us, which uses a custom designed silicone plug for bone replacement after minimally invasive skull base surgery with a low incidence of postoperative CSF leaks. German Landrace pigs were used to test the efficiency of the new technique. Twelve craniotomies were performed in six pigs using a subtemporal approach and…

RNA marker modifications reveal the necessity for rigorous preparation protocols to avoid artifacts in epitranscriptomic analysis

2021

Abstract The accurate definition of an epitranscriptome is endangered by artefacts resulting from RNA degradation after cell death, a ubiquitous yet little investigated process. By tracing RNA marker modifications through tissue preparation protocols, we identified a major blind spot from daily lab routine, that has massive impact on modification analysis in small RNAs. In particular, m6,6A and Am as co-varying rRNA marker modifications, appeared in small RNA fractions following rRNA degradation in vitro and in cellulo. Analysing mouse tissue at different time points post mortem, we tracked the progress of intracellular RNA degradation after cell death, and found it reflected in RNA modific…

AP-1 Transcription Factor Serves as a Molecular Switch between Chlamydia pneumoniae Replication and Persistence

2015

ABSTRACT Chlamydia pneumoniae is a Gram-negative bacterium that causes acute or chronic respiratory infections. As obligate intracellular pathogens, chlamydiae efficiently manipulate host cell processes to ensure their intracellular development. Here we focused on the interaction of chlamydiae with the host cell transcription factor activator protein 1 (AP-1) and its consequence on chlamydial development. During Chlamydia pneumoniae infection, the expression and activity of AP-1 family proteins c-Jun, c-Fos, and ATF-2 were regulated in a time- and dose-dependent manner. We observed that the c-Jun protein and its phosphorylation level significantly increased during C. pneumoniae development.…

Enhancing Gene Knockdown Efficiencies by Comparing siRNA-Loaded Cationic Nanogel Particles of Different Sizes

2015

lt;pgt;In order to silence the expression levels of pathogenic genes, small interfering RNA (siRNA) requires a nano-sized carrier for its safe and stable delivery into cells. In this research highlight, we focus on well-defined cationic nanohydrogel particles developed in our group for such purposes. To investigate the nanogels’ mechanism for enhanced knockdown efficiencies, we recently synthesized two sets of particles with similar material composition and siRNA-loading characteristics, but – according to the manufacturing process – of different sizes. Within this study, 100-nm-sized nanogel particles loaded with siRNA accumulated inside the lysosomes already after 4 h and could not induce…