Search results for "INTRAC"
showing 10 items of 1509 documents
Intraflagellar transport protein 172 is essential for primary cilia formation and plays a vital role in patterning the mammalian brain
2008
AbstractIFT172, also known as Selective Lim-domain Binding protein (SLB), is a component of the intraflagellar transport (IFT) complex. In order to evaluate the biological role of the Ift172 gene, we generated a loss-of-function mutation in the mouse. The resulting Slb mutant embryos die between E12.5 and 13.0, and exhibit severe cranio-facial malformations, failure to close the cranial neural tube, holoprosencephaly, heart edema and extensive hemorrhages. Cilia outgrowth in cells of the neuroepithelium is initiated but the axonemes are severely truncated and do not contain visible microtubules. Morphological and molecular analyses revealed a global brain-patterning defect along the dorsal–…
Electron microscopy of a double helical tubular filament in keyhole limpet (Megathura crenulata) hemolymph.
1992
A approximately 25 nm hollow double helical filament has been detected ultrastructurally in the cell-free supernatant from hemolymph of the keyhole limpet Megathura crenulata (Gastropoda: Prosobranchia: Fissurellidae). Subsequently, much higher concentrations of this material were found in the cell pellet from hemolymph. Both negative staining and thin sectioning have been performed in an attempt to obtain a preliminary structural characterization of this new filament. It is proposed that the filaments are released or secreted from blood hemocytes in response to bleeding, but it has not been possible to define absolutely an intracellular organelle containing this material. It is shown that …
Käsivedon muuttujien yhteys viidenkymmenen metrin vapaauinnin aikaan : uuden anturiteknologiaan perustuvan tehomittarin testaus
2017
Johdanto. Uimari on uidessaan käytännössä lähes koko vartalollaan kosketuksissa veteen. Vesi on väliaine, joka vastustaa uimarin etenemistä ja josta voiman ja vastavoiman periaatteen mukaisesti uimaria eteenpäin vievien voimien tukipinnat tulisi löytää. Veden viskoelastinen luonne luo omat erityispiirteensä eteenpäin suuntautuvien ja vastustavien voimien välille. Tässä tutkimuksessa keskitytään vapaauinnin käsivedon muuttujiin, joiden avulla pyritään arvioimaan uimarin käsivedon eteenpäin vieviä voimia ja niiden yhteyttä uintinopeuteen. Tämän lisäksi tutkitaan anturiteknologiaan perustuvan tehomittarin reliabiliteettia ja arvioidaan validiteettia. Menetelmät. Koehenkilöinä oli kansallisen j…
Pacientu ar ventrikulāro stomu aprūpe intensīvās terapijas un reanimācijas nodaļā
2018
Bakalaura darba tēma ir “Pacientu ar ventrikulāro stomu aprūpe intensīvās terapijas un reanimācijas nodaļā”. Smaga galvas smadzeņu trauma visbiežāk ir 15-35 gadu veciem cilvēkiem, un tā ir 1/3 ar traumatismu saistītu nāves gadījumu. Pieejamā medicīnas statistika liecina, ka gadā no katriem 100.000 iedzīvotājiem 150-200 tiek ievietoti stacionārā intensīvās terapijas nodaļā, galvas traumas dēļ. No hospitalizētiem pacientiem 8-11% ir galvas trauma ir ar 30-60% letalitāti. Smagas galvas traumas rada divējādus bojājumus – primārus un sekundārus, kas ir viens no nāves un invaliditātes cēloņiem pacientiem līdz 40 gadu vecumam, kā arī, galvenais, kvalitatīvas dzīves gadu zaudējuma iemesls. Tēmas ak…
Multicenter Consistency Assessment of Valvular Flow Quantification With Automated Valve Tracking in 4D Flow CMR
2021
OBJECTIVES This study determined: 1) the interobserver agreement; 2) valvular flow variation; and 3) which variables independently predicted the variation of valvular flow quantification from 4-dimensional (4D) flow cardiac magnetic resonance (CMR) with automated retrospective valve tracking at multiple sites. BACKGROUND Automated retrospective valve tracking in 4D flow CMR allows consistent assessment of valvular flow through all intracardiac valves. However, due to the variance of CMR scanners and protocols, it remains uncertain if the published consistency holds for other clinical centers. METHODS Seven sites each retrospectively or prospectively selected 20 subjects who underwent whole …
Glutathione in Cancer Cell Death
2011
Glutathione (L-γ-glutamyl-L-cysteinyl-glycine; GSH) in cancer cells is particularly relevant in the regulation of carcinogenic mechanisms; sensitivity against cytotoxic drugs, ionizing radiations, and some cytokines; DNA synthesis; and cell proliferation and death. The intracellular thiol redox state (controlled by GSH) is one of the endogenous effectors involved in regulating the mitochondrial permeability transition pore complex and, in consequence, thiol oxidation can be a causal factor in the mitochondrion-based mechanism that leads to cell death. Nevertheless GSH depletion is a common feature not only of apoptosis but also of other types of cell death. Indeed rates of GSH synthesis and…
Changes in the Pi uptake and polyP accumulation in Saccharomyces cerevisiae strains deficient in the synthesis of trehalose and/or glycerol
2007
Abstract The intracellular level of free inorganic orthophosphate (P i ) in yeast cells generally depends on the P i uptake capacity, energy state of the cells in respect to the activity of the membrane-associated ATPases and on the activity of metabolic pathways involved in the production of glycerol and trehalose. Batch fermentation was performed to investigate the carbon substrate consumption, the P i uptake capacity and product formation by four Saccharomyces cerevisiae strains differing in their ability to produce glycerol and/or trehalose. The consumption of P i in mutant strains with a lack of the synthesis of the trehalose and/or glycerol exceeded the level for a wild type strain ab…
pH Homeostasis and Citric Acid Utilization: Differences Between Leuconostoc mesenteroides and Lactococcus lactis
1997
This study presents the effects of citric acid and extracellular pH (pHe) on the intracellular pH (pHi) of wild-type and citrate negative variants (cit−) Leuconostoc mesenteroides subsp. mesenteroides (Ln. mesenteroides M) and Lactococcus lactis subsp. lactis bv. diacetylactis (L. lactis LD). A recent method using a pH-sensitive fluorescent indicator carboxyfluorescein succinimidyl ester (cFSE) was adapted to measure the pHi of these two lactic acid bacteria in resting cells. Energized cells with 10 mM lactose of Ln. mesenteroides M and L. lactis LD modified their pH gradient (ΔpH) in the same manner; when the pHe was decreased from 7 to 4, the pHi decreased from 7 to about 5. The adjunctio…
Influence of lactose-citrate co-metabolism on the differences of growth and energetics in Leuconostoc lactis, Leuconostoc mesenteroides ssp. mesenter…
1999
International audience; The biodiversity of growth and energetics in Leuconostoc sp. has been studied in MRS lactose medium with and without citrate. On lactose alone, Ln. lactis has a growth rate double that of Ln. cremoris and Ln. mesenteroides. The pH is a more critical parameter for Ln. mesenteroides than for Ln. lactis or Ln. cremoris; without pH control Ln. mesenteroides is unable to acidify the medium under pH 4.5, while with pH control and as a consequence of a high Y(ATP) its growth is greater than Ln. lactis and Ln. cremoris. In general, lactose-citrate co-metabolism increases the growth rate, the biomass synthesis, the lactose utilisation ratio, and the production of lactate and …
Onset of DNA synthesis in experimentally activated ascidian eggs
1994
DNA synthesis was studied autoradiographically in unfertilized ascidian eggs (Ascidia malaca and Ciona intestinalis) that had been artificially activated by a K+-free external medium or a Ca-ionophore. Naked eggs of A. malaca were incubated in K+-free seawater that contained [3H]-thymidine for 30 min, and naked eggs of C. intestinalis were incubated in seawater supplemented with a Ca-ionophore and [3H]-thymidine for 15 min. The observations revealed limited activation in eggs treated with K+-free seawater. Activation consisted of surface modifications and the onset of DNA synthesis. Incorporation of [3H]-thymidine was detected in female nuclei of treated eggs which had not ejected their pol…