Search results for "ISIA"
showing 10 items of 1032 documents
Network motif-based analysis of regulatory patterns in paralogous gene pairs
2020
Current high-throughput experimental techniques make it feasible to infer gene regulatory interactions at the whole-genome level with reasonably good accuracy. Such experimentally inferred regulatory networks have become available for a number of simpler model organisms such as S. cerevisiae, and others. The availability of such networks provides an opportunity to compare gene regulatory processes at the whole genome level, and in particular, to assess similarity of regulatory interactions for homologous gene pairs either from the same or from different species. We present here a new technique for analyzing the regulatory interaction neighborhoods of paralogous gene pairs. Our central focu…
Surviving the heat: heterogeneity of response inSaccharomyces cerevisiaeprovides insight into thermal damage to the membrane
2015
Environmental heat stress impacts on the physiology and viability of microbial cells with concomitant implications for microbial activity and diversity. Previously, it has been demonstrated that gradual heating of Saccharomyces cerevisiae induces a degree of thermal resistance, whereas a heat shock results in a high level of cell death. Here, we show that the impact of exogenous nutrients on acquisition of thermal resistance differs between strains. Using single-cell methods, we demonstrate the extent of heterogeneity of the heat-stress response within populations of yeast cells and the presence of subpopulations that are reversibly damaged by heat stress. Such cells represent potential for…
Expression profile of genes involved in hydrogen sulphide liberation by Saccharomyces cerevisiae grown under different nitrogen concentrations
2009
AbstractThe present work aims to elucidate molecular mechanisms underlying hydrogen sulphide production in S. cerevisiae associated to nitrogen deficiency. To assess, at a genome-wide level, how the yeast strain adapted to the progressive nitrogen depletion and to nitrogen re-feeding, gene expression profiles were evaluated during fermentation at different nitrogen concentrations, using the DNA array technology. The results showed that most MET genes displayed higher expression values at the beginning of both control and N-limiting fermentation, just before the time at which the release of sulphide was observed. MET genes were downregulated when yeast stopped growing which could associate M…
Antiproliferative activity of green, black tea and olive leaves polyphenols subjected to biosorption and in vitro gastrointestinal digestion in Caco-…
2020
Olive (Olea europaea L.) leaves and tea (Camellia sinensis) are rich sources of bioactive compounds, especially polyphenols. Our previous studies have evidenced the potential use of Saccharomyces cerevisiae as a natural delivery system for these antioxidants and a means to improve their bioaccessibility in the human gut. In the present work, the antiproliferative effect of green tea (GT), black tea (BT) and olive leaves (OL) infusions and suspensions of S. cerevisiae were evaluated, for the first time, in human colon cancer cells (Caco-2) after biosorption and in vitro gastrointestinal digestion. The bioaccessible fractions (BF) were not overtly cytotoxic, not affecting cell viability. ROS …
Denboratik kausara: -nez gero kausazko lokailuaren garapenaz
2019
LABURPENA Lan honetan -nez gero atzizki multzoaren garapena dugu aztergai, lehen testuetatik hasi eta gaurdaino. Denbora lokailuetatik sortu diren beste kausazko lokailu asko bezala, -nez gero batez ere mintza-ekintzak eta antzekoak justifikatzeko erabiltzen da. Hala ere, kausa neutroagoak adierazteko ere erabiltzen da. Gaztelaniaz ez bezala, menpeko perpausaren kokagunea ez da aldatu azken mendeotan eta funtzio informatiboan ere ez dugu aldakuntza handirik ikusten. Sumatzen dugun garapen nagusia da beharbada edukizko kausazko neutroetan maizago erabiltzeko joera. Oro har, -nez gero kausazko menderagailuak, gazt. ya que-rekin baino, antz handiagoa du fr. puisque eta ing. since lokailuekin. …
SICILIAN NATURALISTIC NEWS: 11 Aclista alticollis; 12 Mycomya (Mycomya) prominens; 13 Empis (Leptempis) confusa; 14 Sciapus platypterus; 15 Myopa pic…
2023
SICILIAN NATURALISTIC NEWS: 11 Aclista alticollis; 12 Mycomya (Mycomya) prominens; 13 Empis (Leptempis) confusa; 14 Sciapus platypterus; 15 Myopa picta; 16 Diaea dorsata; 17 Franklinothrips megalops; 18 Dasyhelea bilineata; 19 Forcipomyia (Synthridomyia) murina; 20 Forcipomyia (Euprojoannisia) psilonota; 21 Incertana drepanensis
Post-Transcriptional Regulation of Iron Homeostasis in Saccharomyces cerevisiae
2013
Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox cofactor in a wide variety of biological processes. Recent studies in Saccharomyces cerevisiae have shown that in response to iron deficiency, an RNA-binding protein denoted Cth2 coordinates a global metabolic rearrangement that aims to optimize iron utilization. The Cth2 protein contains two Cx8Cx5Cx3H tandem zinc fingers (TZFs) that specifically bind to adenosine/uridine-rich elements within the 3' untranslated region of many mRNAs to promote their degradation. The Cth2 protein shuttles between the nucleus and the cytoplasm. Once inside the nucleus, Cth2 binds target mRNAs and stimulate…
Transcriptional Response of Saccharomyces cerevisiae to Different Nitrogen Concentrations during Alcoholic Fermentation▿ †
2007
Gene expression profiles of a wine strain of Saccharomyces cerevisiae PYCC4072 were monitored during alcoholic fermentations with three different nitrogen supplies: (i) control fermentation (with enough nitrogen to complete sugar fermentation), (ii) nitrogen-limiting fermentation, and (iii) the addition of nitrogen to the nitrogen-limiting fermentation (refed fermentation). Approximately 70% of the yeast transcriptome was altered in at least one of the fermentation stages studied, revealing the continuous adjustment of yeast cells to stressful conditions. Nitrogen concentration had a decisive effect on gene expression during fermentation. The largest changes in transcription profiles were o…
Caracterización enológica y molecular de híbridos naturales y artificiales entre las especies saccharomyces cerevisiae y saccharomyces kudriavzevii
2018
La industria enológica debe dar respuesta a las nuevas demandas del consumidor, así como responder ante los cambios en la materia prima(mosto) debidos al cambio climático, de gran repercusión en la calidad y aceptación del vino. Para ello se exigen cambios en las prácticas enológicas como el desarrollo de cultivos iniciadores de levaduras adaptadas a las nuevas condiciones de fermentación como son las bajas temperaturas para obtener vinos más aromáticos o el desarrollo de levaduras que produzcan más aromas, mayor concentración de glicerol y menos etanol. S. cerevisiae se ha descrito como la especie predominante en la fermentación alcohólica; sin embargo, otras especies del género Saccharomy…
Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an -L-Rhamnosidase of enological in…
2003
Monoterpenes such as geraniol, linalool, and -terpineol present in grapes determine the varietal flavor properties of young quality wines made from Muscat varieties (for reviews, see references 19 and 21). Geraniol and linalool are considered to be the most important of the monoterpene alcohols, as they are present in greater concentrations and have lower flavor thresholds than other major wine monoterpenes. In particular, linalool is thought to be responsible for the grapelike aroma of wines produced from the Muscat variety. A large proportion of