Search results for "ISR"

Cell stimulation versus cell death induced by sequential treatments with pulsed electric fields and cold atmospheric pressure plasma

2018

Pulsed electric fields (PEFs) and cold atmospheric pressure plasma (CAP) are currently both investigated for medical applications. The exposure of cells to PEFs can induce the formation of pores in cell membranes and consequently facilitate the uptake of molecules. In contrast, CAP mainly acts through reactive species that are generated in the liquid environment. The objective of this study was to determine, if PEFs combined with plasma-treated cell culture medium can mutually reinforce effects on viability of mammalian cells. Experiments were conducted with rat liver epithelial WB-F344 cells and their tumorigenic counterpart WB-ras for a direct comparison of non-tumorigenic and tumorigenic…

An in vitro investigation on the cytotoxic and nuclear receptor transcriptional activity of the mycotoxins fumonisin B1 and beauvericin.

2016

Fumonisin B1 (FB1) and beauvericin (BEA) are secondary metabolites of filamentous fungi, which under appropriate temperature and humidity conditions may develop on various foods and feeds. To date few studies have been performed to evaluate the toxicological and endocrine disrupting effects of FB1 and BEA. The present study makes use of various in vitro bioassays including; oestrogen, androgen, progestagen and glucocorticoid reporter gene assays (RGAs) for the study of nuclear receptor transcriptional activity, the thiazolyl blue tetrazolium bromide (MTT) assay to monitor cytotoxicity and high content analysis (HCA) for the detection of pre-lethal toxicity in the RGA and Caco-2 human colon …

Bisphenolic compounds alter gene expression in MCF-7 cells through interaction with estrogen receptor α

2020

Plasticizers released from microplastic are increasingly viewed with concern. While adverse health effects induced by bisphenol A and its analogues on marine animals are well documented in the literature, the endocrine potential of bisphenolic compounds on human health remains elusive. We applied next generation sequencing (NGS) with the estrogen receptor α (ERα) positive human breast cancer cell line MCF-7 treated with 17-β-estradiol (E2), bisphenol A (BPA), bisphenol B (BPB), bisphenol Z (BPZ) and tetramethyl bisphenol A (4MeBPA). We used molecular docking, microscale thermophoresis, ERα activation assay, and cell cycle experiments on MCF-7 and ERα overexpressing HEK293 cells to verify th…

Regulatory identification of BPA as an endocrine disruptor : Context and methodology

2018

International audience; BPA is one of the most investigated substances for its endocrine disruptor (ED) properties and it is at the same time in the center of many ED-related controversies, the analysis on how BPA fits to the regulatory identification as an ED is a challenge in terms of methodology. It is also a great opportunity to test the regulatory framework with a uniquely data-rich substance and learn valuable lessons for future cases. From this extensive database, it was considered important to engage in a detailed analysis so as to provide specific and strong evidences of ED while reflecting accurately the complexity of the response as well the multiplicity of adverse effects. An ap…

The Role of the Bisphenol A in Diabetes and Obesity

2021

Bisphenol A is a compound commonly found in products meant for daily use. It was one of the first compounds to be identified as an endocrine disruptor that was capable of disrupting the endocrine system and producing very similar effects to those of metabolic syndrome. It has recently gained popularity in the scientific arena as a risk factor for obesity and diabetes due to its ability to imitate natural oestrogens and bind to their receptors. The aim was to study the possible relationship between the Bisphenol A endocrine disruptor with diabetes and obesity. The analysis of the articles allows us to conclude that Bisphenol A is an additional risk factor to consider in the development of di…

The moral work of becoming a professional

2021

Abstract In contemporary working life, art-based initiatives are increasingly used in organizational training and development. For artists, this has created new employment opportunities as creative entrepreneurs who provide specialist services for workplaces. In this article, we study the dynamics of such encounters through the narrated accounts of training professionals. Our data come from a professional mentoring program where the working pairs of artists and consultants shared stories about their customer projects. By using conversation analysis as a method, we analyze the way stories are interactionally accomplished in peer group sessions of the program. In particular, we analyze how pa…

Reviving the lost spaces under urban highways and bridges: an empirical study

2019

Purpose The fast development of urban movement infrastructures has created neglected urban places in cities. This study aims to provide users’ preferences for designing lost spaces that are a by-product of elevated urban highways (UHs) and bridges to develop a conceptual model for better environmental design. Design/methodology/approach This research is conducted by a combination of both qualitative and quantitative methods. In the first phase, to explore the citizen’s environmental preferences based on the Q-sort technique and in-depth interviews, the ideas of 50 users were considered up to data saturation. The preferences of people for designs under urban bridges were extracted by conten…

Reducing disruptive behaviours and improving classroom behavioural climate with class-wide positive behaviour support in middle schools

2017

Disruptive behaviour in classrooms is a significant challenge for learning in schools and a risk factor for students’ academic achievement and a significant source of teachers’ work-related stress. Earlier research shows that clear behavioural expectations, monitoring students’ adherence to them and behaviour-specific praise are effective practices to reduce disruptive behaviour. Although behaviour problems are common in middle schools, most of the interventions have been developed and studied in elementary schools. This randomised study evaluated the effects of a class-wide intervention on classroom behavioural climate and disruptive behaviour, on teacher-experienced stress and on the time…

Television musiikkiohjelmat 1958-1972 : televisio määrällisenä musiikkisivistäjänä

2008

Benefits of soil carbon: report on the outcomes of an international scientific committee on problems of the environment rapid assessment workshop

2014

The outcomes of the discussion in the four working sessions showed that although there is an urgent need to improve soil carbon management and stocks, and despite the existing knowledge about good agricultural practices to achieve this goal, these are not put into practice effectively and globally. The apparent contradiction has to do with a mismatch of policies at different societal and geographical scales, and the low policy profile of SOC. All participants agreed in the need to bring SOC into the core of environmental policies at all levels and to improve the governance of policy actions by addressing the stakeholders in a more effective way. Fil: Banwart, Steven. University of Sheffield…