Search results for "ITO"

showing 10 items of 25573 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
researchProduct

Influence of salt of different origin on the microbiological characteristics, histamine generation and volatile profile of salted anchovies (Engrauli…

2018

Abstract The effect of six salts of different geographical areas on the quality of salted anchovies was evaluated. The crude salts were chemically characterized by determination of inorganic and volatile organic compounds (VOCs). Salted anchovies, corresponding to six experimental trials, were subjected to microbiological, chemical (including histamine content) and sensory analysis during the entire period of ripening (150 days). The salts were characterized by marked differences in terms of major cations and trace element amounts. Among the 27 VOCs detected, octadecane was the most abundant compound and the main differences of the salts were registered for alkanes and alcohols. During matu…

0106 biological sciences0301 basic medicineTasteSaltSettore AGR/13 - Chimica Agraria030106 microbiologySalt (chemistry)01 natural sciencesSensory analysis03 medical and health scienceschemistry.chemical_compoundEngraulis010608 biotechnologyAnchovieFood scienceSettore BIO/06 - Anatomia Comparata E CitologiaStaphylococcaceaechemistry.chemical_classificationbiologyChemistryVOCRipeningbiology.organism_classificationLactic acidComposition (visual arts)Bacterial communityHistamineSettore AGR/16 - Microbiologia AgrariaFood ScienceBiotechnologyFood Control
researchProduct

Contrasting biogeographical patterns in Margarella (Gastropoda: Calliostomatidae: Margarellinae) across the Antarctic Polar Front

2021

International audience; Members of the trochoidean genus Margarella (Calliostomatidae) are broadly distributed across Antarctic and sub-Antarctic ecosystems. Here we used novel mitochondrial and nuclear gene sequences to clarify species boundaries and phylogenetic relationships among seven nominal species distributed on either side of the Antarctic Polar Front (APF). Molecular reconstructions and species-delimitation analyses recognized only four species: M. antarctica (the Antarctic Peninsula), M. achilles (endemic to South Georgia), M. steineni (South Georgia and Crozet Island) and the morphologically variable M. violacea (=M. expansa, M. porcellana and M. pruinosa), with populations in s…

0106 biological sciences0301 basic medicineTime FactorsAntarctic Polar FrontGastropodalong-distance dispersalAntarctic RegionsBiology010603 evolutionary biology01 natural sciencesDNA MitochondrialPorcellanaCalliostomatidae03 medical and health sciencesSpecies SpecificityGastropodaGeneticsAnimals14. Life underwaterGlacial periodMargarellaSouthern OceanMolecular BiologyEcology Evolution Behavior and SystematicsPhylogenyPolar frontGenetic diversityPolymorphism GeneticEcologyBayes TheoremDNA15. Life on landSouth Americabiology.organism_classificationbenthic-protected developmentraftingPhylogeographyMESH: Océan Austral front polaire antarctique dispersion à longue distance développement protégé benthique incubation rafting Margarella030104 developmental biologyBiological dispersalTaxonomy (biology)[SDE.BE]Environmental Sciences/Biodiversity and Ecology
researchProduct

Testing the enemy release hypothesis: abundance and distribution patterns of helminth communities in grey mullets (Teleostei: Mugilidae) reveal the s…

2017

International audience; The abundance and aggregation patterns of helminth communities of two grey mullet hosts, Liza haematocheilus and Mugil cephalus, were studied across 14 localities in Atlantic and Pacific marine areas. The analysis matched parasite communities of (i) L. haematocheilus across its native and introduced populations (Sea of Japan and Sea of Azov, respectively) and (ii) the introduced population of L. haematocheilus with native populations of M. cephalus (Mediterranean, Azov-Black and Japan Seas). The total mean abundance (TMA), as a feature of the infection level in helminth communities, and slope b of the Taylor’s power law, as a measure of parasite aggregation at the in…

0106 biological sciences0301 basic medicineTotal mean abundancePopulationSpecies distributionIntroduced species010603 evolutionary biology01 natural sciencesHost SpecificityInvasive speciesFish Diseases03 medical and health sciencesSpecies SpecificityAbundance (ecology)AnimalsHelminths[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology14. Life underwatereducationeducation.field_of_studybiologyMugil cephalusEcologyMugilHost (biology)030108 mycology & parasitologybiology.organism_classificationTaylor’s lawSmegmamorphaInfectious DiseasesSpecies abundance distributionParasitologyLiza haematocheilusHelminthiasis AnimalIntroduced SpeciesAnimal Distribution
researchProduct

Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants

2018

Abstract As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel, 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. The European Commission has mandated EFSA to develop a technical note to the applicants on, and checking of, the quality of the methodology, analysis and reporting covering complete sequencing of the insert and flanking regions, insertion site analysis of the GM event, and generational stability and integrity. This Technical Note puts together requirements and r…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]2405 Parasitologymolecularcharacterisationnext‐generation sequencingContext (language use)Plant ScienceComputational biologyGenetically modified cropsBiology01 natural sciencesMicrobiologyGenomeInsert (molecular biology)DNA sequencing03 medical and health sciencessymbols.namesake1110 Plant Sciencegenetically modified organismgenetic stabilityDNA sequencing1106 Food ScienceSanger sequencinggenetically modified organisms2404 Microbiologyrisk assessmentmolecular characterisation10079 Institute of Veterinary Pharmacology and ToxicologyGenetically modified organism3401 Veterinary (miscellaneous)Scientific Opinion030104 developmental biologyNGSsymbols570 Life sciences; biologyAnimal Science and ZoologyParasitologynext-generation sequencing1103 Animal Science and Zoology010606 plant biology & botanyFood ScienceVerification and validation
researchProduct

Assessment of genetically modified cotton GHB614 × LLCotton25 × MON 15985 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFS…

2018

The three-event stack cotton GHB614 x LLCotton25 x MON 15985 was produced by conventional crossing to combine three single cotton events, GHB614, LLCotton25 and MON 15985. The EFSA GMO Panel previously assessed the three single events and did not identify safety concerns. No new data on the single events that could lead to modification of the original conclusions on their safety were identified. Based on the molecular, agronomic, phenotypic and compositional characteristics, the combination of the single events and of the newly expressed proteins in the three-event stack cotton did not give rise to food and feed safety or nutritional issues. Food and feed derived from cotton GHB614 x LLCott…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]Plant ScienceTP1-1185Biology01 natural sciencesMicrobiology03 medical and health sciencesEnvironmental safetyNPTIIGUSCry1AcTX341-641cotton GHB614 × LLCotton25 × MON 15985Cry2Ab2cotton GHB614 x LLCotton25 x MON 159852. Zero hungerAnimal healthbusiness.industryNutrition. Foods and food supplyGMOChemical technologyRegulation (EC) 1829/2003Food safetyGenetically modified organismBiotechnologySettore AGR/02 - Agronomia E Coltivazioni ErbaceeScientific Opinion030104 developmental biologySettore AGR/11 - Entomologia Generale E ApplicataAnimal Science and ZoologyParasitologyGMO; cotton GHB614 x LLCotton25 x MON 15985; Regulation (EC) 1829/2003; Cry1Ac; Cry2Ab2; GUS; NPTII; 2mEPSPS; PATbusiness2mEPSPSPAT010606 plant biology & botanyFood Science
researchProduct

Assessment of genetically modified maize MON 87403 for food and feed uses, import and processing, under Regulation (EC) No 1829/2003 (application EFS…

2018

GMO; maize (Zea mays); MON 87403; ear biomass; Regulation (EC) No 1829/2003; International audience; aize MON 87403 was developed to increase ear biomass at early reproductive phase through the expression of a modified AtHB17 gene from Arabidopsis thaliana, encoding a plant transcription factor of the HD-Zip II family. The molecular characterisation data and bioinformatic analyses did not identify issues requiring assessment for food and feed safety. No statistically significant differences in the agronomic and phenotypic characteristics tested between maize MON 87403 and its conventional counterpart were identified. The compositional analysis of maize MON 87403 did not identify differences…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]Plant ScienceTP1-1185GMO; maize (Zea mays); MON 87403; ear biomass; Regulation (EC) No 1829/2003Biologyear biomass01 natural sciencesMicrobiologyMON 87403[SHS]Humanities and Social Sciences03 medical and health sciencesEnvironmental safetyear bioma[SDV.IDA]Life Sciences [q-bio]/Food engineering[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyTX341-641MON 87403[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineeringmaize (Zea mays)2. Zero hungerGenetically modified maizeAnimal healthbusiness.industryNutrition. Foods and food supplyGMOChemical technologyfungiRegulation (EC) No 1829/2003maize (Zea mays)Food safetyBiotechnologySettore AGR/02 - Agronomia E Coltivazioni Erbacee030104 developmental biologyScientific OpinionSettore AGR/11 - Entomologia Generale E Applicata[SDE]Environmental SciencesAnimal Science and ZoologyParasitologybusiness010606 plant biology & botanyFood ScienceRegulation (EC) No 1829/2003
researchProduct

Gastrointestinal parasites of two populations of Arctic foxes (Vulpes lagopus) from north-east Greenland

2017

Parasitological examination of 275 faecal samples from Arctic foxes (Vulpes lagopus) collected at Zackenberg Valley and Karupelv Valley in north-east Greenland from 2006 to 2008 was conducted using sieving and microscopy. Overall, 125 (45.5%) samples contained parasite eggs of Taenia crassiceps, Taenia serialis, Toxascaris leonina, Eucoleus boehmi, Physalopteridae and Ancylostomatidae, and Strongyloides-like larvae. As long-term ecological studies are conducted at both sampling locations, the present findings constitute a baseline data set for further parasitological monitoring.

0106 biological sciences0301 basic medicineVulpesEucoleus boehmiZoologyOceanography010603 evolutionary biology01 natural sciencesToxascaris leoniana03 medical and health scienceslcsh:OceanographyEarth and Planetary Sciences (miscellaneous)Environmental Chemistry[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyToxascaris leonina14. Life underwaterlcsh:GC1-1581lcsh:Environmental sciencesGeneral Environmental ScienceTaenia crassicepslcsh:GE1-350Toxascaris leoninabiologyAncylostomatidaeTaeniaEcologyfaecal analysis030108 mycology & parasitologybiology.organism_classificationTaenia serialisArcticLagopusTaeniaArctic parasites[SDE.BE]Environmental Sciences/Biodiversity and Ecologycoprological examinationPolar Research
researchProduct

Symbiotic polydnavirus and venom reveal parasitoid to its hyperparasitoids

2018

Symbiotic relationships benefit organisms in utilization of new niches. In parasitoid wasps, symbiotic viruses and venom that are injected together with wasp eggs into the host caterpillar suppress immune responses of the host and enhance parasitoid survival. We found that the virus also has negative effects on offspring survival when placing these interactions in a community context. The virus and venom drive a chain of interactions that includes the herbivore and its food plant and attracts the hyperparasitoid enemies of the parasitoid. Our results shed new light on the importance of symbionts associated with their host in driving ecological interactions and highlight the intricacy of how…

0106 biological sciences0301 basic medicineWasps01 natural sciencesMultitrophic interactionParasitoidHerbivore-induced plant volatilesGene Expression Regulation PlantLaboratory of EntomologyTrophic levelPlant-mediatedLarvaMultidisciplinarybiologyPolydnavirusHerbivore-induced plant volatilePlantsBiological SciencesWaspPE&RCOrganische ChemieBiosystematiekInteraction networkinternationalLarvaSymbiosiButterfliesZoology010603 evolutionary biologyHost-Parasite Interactions03 medical and health sciencesMultitrophic interactionsSymbiosisButterflieAnimalsSymbiosisCaterpillarSalivaEcosystemHerbivoreParasitic waspVenomsHost (biology)AnimalOrganic ChemistryfungiPlantLaboratorium voor Entomologiebiology.organism_classificationVenom030104 developmental biologySettore AGR/11 - Entomologia Generale E ApplicataPolydnaviridaeHerbivoreBiosystematicsEPS
researchProduct

Living in a harsh habitat: epidemiology of the whale louse, Syncyamus aequus (Cyamidae), infecting striped dolphins in the Western Mediterranean

2017

Whale lice (Cyamidae) are ectoparasitic amphipods exclusive to cetaceans. Data on their epidemiology usually come from species infecting baleen whales, which are large, slow-moving hosts. In this study, we provide data on infection parameters, population structure and microhabitat selection in a whale louse exclusive to delphinids, Syncyamus aequus, with the aim of comparing them with those from cyamid species from large whales. A total of 176 striped dolphins Stenella coeruleoalba stranded along the Mediterranean coast of Spain during 1980–2016 were examined. The prevalence of S. aequus was 27.3% (95% CI: 20.9–34.3), and populations were sparse, with a mean number of whale lice per infecte…

0106 biological sciences0301 basic medicineWhalemedia_common.quotation_subjectStenella coeruleoalba030108 mycology & parasitologyBiologyWhale lousebiology.organism_classificationFecundity010603 evolutionary biology01 natural sciencesCompetition (biology)Sexual dimorphismFishery03 medical and health sciencesBaleenbiology.animalAnimal Science and ZoologyEcology Evolution Behavior and SystematicsSex ratiomedia_commonJournal of Zoology
researchProduct