Search results for "In vitro model"

Bioaccessibility of bioactive compounds after non-thermal processing of an exotic fruit juice blend sweetened with Stevia rebaudiana

2017

Abstract A comparative study of the bioaccessibility of bioactive compounds and antioxidant capacity in a fruit juice-Stevia rebaudiana mixture processed by pulsed electric fields (PEF), high voltage electrical discharges (HVED) and ultrasound (USN) technology at two equivalent energy inputs (32–256 kJ/kg) was made using an in vitro model. Ascorbic acid was not detected following intestinal digestion, while HVED, PEF and USN treatments increased total carotenoid bioaccessibility. HVED at an energy input of 32 kJ/kg improved bioaccessibility of phenolic compounds (34.2%), anthocyanins (31.0%) and antioxidant capacity (35.8%, 29.1%, 31.9%, for TEAC, ORAC and DPPH assay, respectively) compared…

DEVELOPMENT OF SECRETOR AND GESTATIONAL PATIENT-DERIVED ORGANOIDS FROM ADENOMYOSIS DISEASE AS AN IN VITRO MODEL TO STUDY ADENOMYOSIS-RELATED INFERTIL…

2021

The toxic effect of monodisperse amorphous silica particles studied on an in vitro model of the human air–blood barrier

2009

Caki-1 Cells Represent an in vitro Model System for Studying the Human Proximal Tubule Epithelium

2007

<i>Background/Aims:</i> The human proximal tubule (PT) epithelium is distinguished from other nephron segments via several unique characteristics. Studies assessing PT epithelium increasingly employ cell lines, bypassing the complexity of primary cell cultures. However, few human model systems exist for studying PT cells in vitro. The current work involves an intensive characterization of Caki-1 cells, a commercially available human renal cell line. <i>Methods:</i> Caki-1 cells were validated as a representative model system for PT cell research via morphological, physiological and biochemical investigations including light and transmission electron microscopy, trans…

Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.

2009

An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secondary to myelin damage, as generally assumed. SPMS serum affects the perme…

Comparative in vitro study of two methods for gingival biotype assessment

2018

Background The gingival thickness seems to have an important role in different dental treatments. There are different methods of quantifying this thickness, but it is not known which of them can be the most effective. The objective to assess the accuracy of two different methods for gingival thickness measurement: the transgingival needle probing (TGNP) and the tension-free caliper (TFC) in an in vitro model, by comparing them with direct physical measurements (reference standard). Material and methods Gingival thickness (GT) was evaluated in 27 female pigs with four implant sites 1, 2 and 3mm from the gingival margin with three different methods: 1) transgingival needle probing 2) tension-…

Iontophoretic in Vitro Release of Antimycotics from Hydrogels

1993

A new in vitro model for iontophoretic release from hydrogels was developed. It represents a modification of the rotary disk cell developed by Moll/Bender and can be used in a normal dissolution tester. The iontophoretic release from antimycotic hydrogels through an artificial membrane was investigated and different types of antimycotics were tested. The influence of current density, drug concentration and vehicle was determined.

A High-Throughput Mechanical Activator for Cartilage Engineering Enables Rapid Screening of in vitro Response of Tissue Models to Physiological and S…

2021

Articular cartilage is crucially influenced by loading during development, health, and disease. However, our knowledge of the mechanical conditions that promote engineered cartilage maturation or tissue repair is still incomplete. Current in vitro models that allow precise control of the local mechanical environment have been dramatically limited by very low throughput, usually just a few specimens per experiment. To overcome this constraint, we have developed a new device for the high throughput compressive loading of tissue constructs: the High Throughput Mechanical Activator for Cartilage Engineering (HiT-MACE), which allows the mechanoactivation of 6 times more samples than current tech…

Neuroprotective properties of xenon and helium in an in vitro model of traumatic brain injury: one small step or one big jump?

2008

Larval zebrafish as an in vitro model for evaluating toxicological effects of mycotoxins.

2020

The presence of mycotoxins in food has created concern. Mycotoxin prevalence in our environment has changed in the last few years maybe due to climatic and other environmental changes. Evidence has emerged from in vitro and in vivo models: some mycotoxins have been found to be potentially carcinogenic, embryogenically harmful, teratogenic, and to generate nephrotoxicity. The risk assessment of exposures to mycotoxins at early life stages became mandatory. In this regard, the effects of toxic compounds on zebrafish have been widely studied, and more recently, mycotoxins have been tested with respect to their effects on developmental and teratogenic effects in this model system, which offers …