Search results for "InSe"
showing 10 items of 3069 documents
Environmental quality assessment of the Sicilian coast using a multi-disciplinary approach
2018
A multidisciplinary approach for the assessment of the environmental quality of the Sicilian coast is presented. This approach can be adopted for any Mediterranean coastline. The territory was divided into elementary areas and for each of them we reported the most important taxa, belonging to fauna and vascular, bryophytic and algal flora, from a biogeographic point of view in order to evaluate punctual and general levels of biodiversity conservation. The comparative analysis showed that the main circumsicilian islands and the carbonate promontories of the Tyrrhenian coast present the highest degree of biodiversity. The coastal lagoons located in the south-eastern Sicily, even though charac…
Comparison of three adhesive systems in class II composite restorations in endodontically treated teeth : influence of Er:YAG laser conditioning and …
2018
Background Dental surface conditioning by Er:YAG laser is currently being investigated, as not all of the mechanisms and effects of this technique have been clearly studied. Thus, the aim of the present study was to assess the cervical microleakage of Class II resin composite restorations in endodontically treated teeth following either the respective conventional conditioning or additional Er:YAG laser conditioning, in association with varied adhesives. Material and methods Standardized mesial-occlusal-distal cavities (two gingival walls positioned in dentin and enamel, respectively) were created in 60 extracted human premolar teeth. Following the completion of the endodontic therapy, the …
Improvement in Nuclear Entry and Transgene Expression of Baculoviruses by Disintegration of Microtubules in Human Hepatocytes
2005
ABSTRACT Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), a potent virus for mammalian cell gene delivery, possesses an ability to transduce mammalian cells without viral replication. We examined the role of the cellular cytoskeleton in the cytoplasmic trafficking of viral particles toward the nucleus in human hepatic cells. Microscopic studies showed that capsids were found in the nucleus after either viral inoculation or cytoplasmic microinjection of nucleocapsids. The presence of microtubule (MT) depolymerizing agents caused the amount of nuclear capsids to increase. Overexpression of p50/dynamitin, an inhibitor of dynein-dependent endocytic trafficking from peripheral e…
Pathways of Cell Infection by Parvoviruses and Adeno-Associated Viruses
2004
Animal viruses have developed various strategies for infecting cells, and all begin with adsorption to cell surface receptors, penetration into the cytosol, uncoating or release of the viral genome, and targeting the genome and any required accessory proteins toward the correct cellular organelle or compartment for replication (26, 48, 63). Since genome delivery and release require the rearrangement of the viral structures, infection is normally a multistep process involving various viral and cellular components. Viruses that replicate in the nucleus must have mechanisms for transporting the genome and other components to the vicinity of the nuclear pore and into the nucleus (84). The endos…
A membrane-destabilizing peptide in capsid protein L2 is required for egress of papillomavirus genomes from endosomes
2005
ABSTRACT Papillomaviruses are internalized via clathrin-dependent endocytosis. However, the mechanism by which viral genomes pass endosomal membranes has not been elucidated. In this report we show that the minor capsid protein L2 is required for egress of viral genomes from endosomes but not for initial uptake and uncoating and that a 23-amino-acid peptide at the C terminus of L2 is necessary for this function. Pseudogenomes encapsidated by L1 and L2 lacking this peptide accumulated in vesicular compartments similar to that observed with L1-only viral particles, and these mutant pseudoviruses were noninfectious. This L2 peptide displayed strong membrane-disrupting activity, induced cytolys…
Structural and functional role of Domain I for the insecticidal activity of the Vip3Aa protein from Bacillus thuringiensis
2022
12 p.-4 fig.-2 tab.
Activation of Bacillus thuringiensis Cry1I to a 50 kDa stable core impairs its full toxicity to Ostrinia nubilalis
2021
Abstract Bacillus thuringiensis Cry1I insecticidal proteins are structurally similar to other three-domain Cry proteins, although their size, activity spectrum, and expression at the stationary phase are unique among other members of the Cry1 family. The mode of action of Cry1 proteins is not completely understood but the existence of an activation step prior to specific binding is widely accepted. In this study, we attempted to characterize and determine the importance of the activation process in the mode of action of Cry1I, as Cry1Ia protoxin or its partially processed form showed significantly higher toxicity to Ostrinia nubilalis than the fully processed protein either activated with …