Search results for "InSe"
showing 10 items of 3069 documents
Developmental profiles of epidermal mRNAs during the pupal-adult molt of Tenebrio molitor and isolation of a cDNA clone encoding an adult cuticular p…
1992
0012-1606 (Print) Journal Article; Changes in translatable mRNAs from the wing epidermis of the Coleoptera Tenebrio molitor have been investigated during metamorphosis by analysis of in vitro translated products. Striking differences between the patterns obtained from mRNAs extracted during pupal and adult cuticle secretion indicated that a drastic change in gene expression occurs during the pupal-adult transition. In addition to these stage-specific modifications, the mRNA patterns changed within each cuticular synthesis program (pupal or adult), especially at ecdysis. After tritiated leucine incorporation, some of the major radiolabeled cuticular proteins showed similar changes suggesting…
Effect of Cry Toxins on Xylotrechus arvicola (Coleoptera: Cerambycidae) Larvae
2022
Simple Summary Xylotrechus arvicola is a destructive pest in vineyards (Vitis vinifera) in the main wine-producing areas of the Iberian Peninsula. X. arvicola larvae bore into the grapevine wood-making galleries, thus damaging the plant both directly and indirectly. The susceptibility of X. arvicola larvae to five coleopteran toxic Cry proteins was evaluated under laboratory conditions in order to deepen the knowledge of the effect of these proteins on this insect throughout its biological development. The Cry proteins tested could be applied to control X. arvicola larvae since they were able to kill them and cause serious alterations in the larvae during the remaining months of development…
External and internal elimination of supernumerary larvae in the whitefly parasitoid Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae)
2008
Abstract The solitary parasitoid Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae) is one of the key biological control agents of the whitefly Bemisia argentifolii Bellows & Perring, also known as B. tabaci (Gennadius) B-biotype (Homoptera: Aleyrodidae). We present new information on its biology, and particularly about larval elimination during the first instar in cases of superparasitism. For the first time, physical elimination of supernumerary larvae was observed, both outside and inside the host. These findings are documented with confocal microscopy images and video recordings. We observed more than 350 B. argentifolii nymphs, parasitized with one, two or more than two larvae. Physi…
Neuropeptidome regulation after baculovirus infection. A focus on proctolin and its relevance in locomotion and digestion
2020
AbstractBaculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues. Some behavioral and physiological changes in lepidopteran larvae have been described following a baculovirus infection and those changes could be connected with alterations in the host’s central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters or neuromodulators. These peptides are involved in regulating animal physiology…
The evolutionary dynamics of adaptive virginity, sex-allocation and altruistic helping in haplodiploid animals
2018
In haplodiploids, females can produce sons from unfertilized eggs without mating. However, virgin reproduction is usually considered to be a result of a failure to mate, rather than an adaptation. Here, we build an analytical model for evolution of virgin reproduction, sex-allocation, and altruistic female helping in haplodiploid taxa. We show that when mating is costly (e.g., when mating increases predation risk), virginity can evolve as an adaptive female reproductive strategy. Furthermore, adaptive virginity results in strongly divergent sex-ratios in mated and virgin queen nests ("split sex ratios"), which promotes the evolution of altruistic helping by daughters in mated queen nests. H…
Nucleotide sequence of an adult-specific cuticular protein gene from the beetle Tenebrio molitor: effects of 20-hydroxyecdysone on mRNA accumulation.
1993
0962-1075 (Print) Journal Article Research Support, Non-U.S. Gov't; The accumulation of transcripts from two adult-specific cuticular genes (ACP-20 and ACP-22) is shown to be modified after addition of exogenous 20-hydroxyecdysone. In the continuous presence of high levels of the hormone, the expression of ACP-20 gene is significantly weaker than that of untreated controls, while ACP-22 expression is 2.5-fold increased. During active synthesis of the ACP messages, a 0.5 microg 20-hydroxyecdysone injection causes a rapid 2-fold increase in ACP-22 mRNA and is not able to repress ACP-20 mRNA accumulation. We conclude that these genes whose transcripts appear in an almost coordinated manner in …
A proctolin-like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion
2022
Baculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues, included the central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters, or neuromodulators. These peptides are involved in regulating animal physiology and behavior and could be altered after baculovirus infection. In this study, we have investigated the effect of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) infection …
A Protein Nuclear Extract fromD. melanogasterLarval Tissues
2008
Preparation of protein nuclear extracts is often the first step to study in vitro biological processes occurring in the nucleus of the eukaryotic cell. Nuclear extracts have been extensively used in different model organisms to identify and study protein function in nuclei. Drosophila embryos can be collected in large quantities and have been the source of choice for the production of protein nuclear extracts. However, most of Drosophila in vivo studies on protein function are conducted in larval tissues. Here we report a new method to produce highly stable large-scale protein nuclear extracts from whole Drosophila larvae that are suited for a variety of biochemical analyses.
Nuevos fármacos inspirados en Annonáceas
2014
Los metabolitos secundarios activos (MSA) juegan un papel importante en el descubrimiento de nuevos medicamentos. Moléculas naturales con esqueletos complejos, tales como las estatinas aisladas de Aspergillus terreus, o las acetogeninas específicas de la familia Annonaceae, no hubieran podido ser inventadas en ningún laboratorio. Los MSA aislados en Annonaceae, especialmente las acetogeninas y los alcaloides isoquinoleínicos, pueden ser considerados como fuente constante de inspiración para químicos, farmacólogos y para todos los investigadores interesados en el descubrimiento de una nueva familia de medicamentos. Active secondary metabolites (ASM) play a highly significant role in the drug…
On the design of optimally inserted transmembrane helices
2017
La célula es la unidad básica estructural y funcional de la vida y todas las células están rodeadas y delimitadas por sus membranas biológicas. Las membranas juegan un papel crucial en la existencia de las células delimitándolas y conectándolas con su entorno. Las membranas biológicas están formadas principalmente por lípidos y proteínas. Mientras que los lípidos forman una bicapa que impide la difusión libre de la mayoría de moléculas e iones, las proteínas controlan el tráfico molecular y el flujo de información a través de la membrana. A estas proteínas insertadas en la membrana biológica se les denomina proteínas de membrana, caracterizadas por la presencia de regiones adaptadas para in…