Search results for "Indel"

Reliability of mitochondrial DNA in an acanthocephalan: The problem of pseudogenes

2006

The utility of mitochondrial DNA as a molecular marker for evolutionary studies is well recognized. However, several problems can arise when using mitochondrial DNA, one of which is the presence of nuclear mitochondrial pseudogenes, or Numts. Pseudogenes of cytochrome oxidase I were preferentially amplified from Acanthocephalus lucii (Acanthocephala) using a universal PCR approach. To verify the presence and abundance of pseudogenes, length heterogeneity analysis of the PCR fragments was performed. PCR products obtained with universal primers often contained fragments of different sizes. Cloned sequences from universal PCR products nearly always contained sequence abnormalities such as inde…

Viral Mutation Rates

2010

Accurate estimates of virus mutation rates are important to understand the evolution of the viruses and to combat them. However, methods of estimation are varied and often complex. Here, we critically review over 40 original studies and establish criteria to facilitate comparative analyses. The mutation rates of 23 viruses are presented as substitutions per nucleotide per cell infection (s/n/c) and corrected for selection bias where necessary, using a new statistical method. The resulting rates range from 108 to106 s/n/c for DNA viruses and from 106 to 104 s/n/c for RNA viruses. Similar to what has been shown previously for DNA viruses, there appears to be a negative correlation between mut…

P58 Differential molecular diagnosis of uterine leiomyomas and leiomyosarcomas using DNA and RNA sequencing

2019

Introduction/Background Nowadays, the absence of standardized criteria to identify and differentiate uterine leiomyomas (LM) and leiomyosarcomas (LMS) prior to surgery, cause a significant stress in the patient, leading to unnecessary invasive procedures and additional costs to the National Health System. As consequence, the development of an accurate and non-invasive differential diagnostic methods in patients with surgical indication is needed to avoid the potential dissemination of hidden LMS from morcellation. We aim to identify differential genetic targets in LMS vs LM using Next Generation Sequencing to advance our knowledge in their differential diagnosis. Methodology A total of 13 L…

Phragmén-Lindelöf's and Lindelöf's theorems

1985

Analytic solutions of the Navier-Stokes equations

2001

We consider the time dependent incompressible Navier-Stokes equations on an half plane. For analytic initial data, existence and uniqueness of the solution are proved using the Abstract Cauchy-Kovalevskaya Theorem in Banach spaces. The time interval of existence is proved to be independent of the viscosity.

The transcriptome of Spodoptera exigua larvae exposed to different types of microbes.

2012

We have obtained and characterized the transcriptome of Spodoptera exigua larvae with special emphasis on pathogen-induced genes. In order to obtain a highly representative transcriptome, we have pooled RNA from diverse insect colonies, conditions and tissues. Sequenced cDNA included samples from 3 geographically different colonies. Enrichment of RNA from pathogen-related genes was accomplished by exposing larvae to different pathogenic and non-pathogenic microbial agents such as the bacteria Bacillus thuringiensis, Micrococcus luteus, and Escherichia coli, the yeast Saccharomyces cerevisiae, and the S. exigua nucleopolyhedrovirus (SeMNPV). In addition, to avoid the loss of tissue-specific …

A new class of spaces with all finite powers Lindelof

2013

We consider a new class of open covers and classes of spaces defined from them, called "iota spaces". We explore their relationship with epsilon-spaces (that is, spaces having all finite powers Lindelof) and countable network weight. An example of a hereditarily epsilon-space whose square is not hereditarily Lindelof is provided.

Radó–Kneser–Choquet theorem

2014

We present a new approach to the celebrated theorem of Rado–Kneser–Choquet (RKC) on univalence of planar harmonic mappings. The novelty lies in establishing a continuous path (isotopy) from the given harmonic map to a conformal one. Along this path the mappings retain positive Jacobian determinant by virtue of so-called Minimum Principle. These ideas extend to nonlinear uncoupled systems of partial differential equations, as in Iwaniec, Koski and Onninen [‘Isotropic p-harmonic systems in 2D, Jacobian estimates and univalent solutions’, Rev. Mat. Iberoam, to appear]. Unfortunately, details of such digression would lead us too far afield. Nonetheless, one gains (in particular) the RKC-Theorem…

The Third Main Theorem

1998

The Second Main Theorem

1998