Search results for "Infant Formula"
showing 10 items of 68 documents
Calcium bioavailability in human milk, cow milk and infant formulasâcomparison between dialysis and solubility methods
1999
Abstract The percentages of total, soluble and dialysable calcium of human milk, cow milk and milk and soy based infant formulas were determined in order to detect possible differences in the calcium bioavailability of the samples. For this purpose an in vitro method was applied to these four calcium sources. The ranking of the analysed samples in terms of calcium bioavailability depends on the criteria applied. Calcium ranked dialysis percentage was: cow milk>human milk>soy based formula>milk based formulas. Calcium ranked solubility percentage was: human milk>cow milk>soy-based formula>milk-based formulas. Comparison of the results of the in vitro assay with the information available on i…
Evaluation of nutritional parameters in infant formulas and powdered milk by Raman spectroscopy
2007
It has been made a critical evaluation of the application of near infrared Fourier transform-Raman spectroscopy for the simultaneous determination of the most important nutritional parameters such as energetic value, carbohydrate, protein and fat contents of infant formula and powdered milk samples based on the use of partial least squares (PLS) regression analysis. A highly heterogeneous population of 23 samples, covering a wide range of infant food formula and powdered milk, were obtained from the Spanish market. Raman spectra, obtained by excitation with a Nd:YAG laser at 1064 nm, show no disturbing fluorescence effects; therefore sample spectra can be recorded without any previous prepa…
Calcium dialysability as an estimation of bioavailability in human milk, cow milk and infant formulas
1999
Abstract The calcium dialysability of human milk, cow milk and infant formulas is estimated by applying the in vitro method (Miller, Schricker, Rasmussen, & Van Campen, (1981). American Journal of Clinical Nutrition, 34, 2248–2256) slightly modified, as a measure of its bioavailability. The influence of the protein composition of infant formula samples on calcium bioavailability is also studied. Whole cow milk, a pool of human milk and 18 commercial infant formulas of 6 different types are analysed. The highest values of calcium dialysability (mg Ca/100 ml infant formula) correspond to premature (13.6) and follow-up formulas (11.8) and the lowest ones to adapted formulas (3.7); the latter v…
Review: Determination of Vitamin D in Dairy Products by High Performance Liquid Chromatography
2005
This work reviews the methods used for the determination of vitamin D in some dairy products (milk and infant formulas) by high performance liquid chromatography (HPLC). The low vitamin D contents and the presence of interfering compounds require sample treatment and purification of the extract. The advantages and drawbacks of hot and cold saponification, direct extraction and different types of extract purification are also discussed, taking into account the lack of vitamin D stability by heating, exposure to light and oxidation. With respect to chromatographic determination, the mode (normal or reverse phase), type of column (microcolumn or conventional) and detection system (UV, electro…
High-performance liquid chromatographic determination of furfural compounds in infant formulas
2002
Furfural contents in adapted and follow-up infant formulas were measured by RP-HPLC. The evolution of furfural compound contents during storage (a year at 20 and 37 °C) was studied. 2-Furylmethylketone and 5-methyl-2-furaldehyde were not detectable in analysed samples. The differences in the furfural compounds at point zero between both infant formulas has to be ascribed to the differences in protein and iron contents. An increase in free 5-hydroxymethyl-2-furfuraldehyde (HMF), 2-furaldehyde (F) and HMF+F contents was observed in all samples, although the differences were not statistically significant. The storage temperature affected the total HMF content and the storage time affected the …
Stability of tocopherols in adapted milk-based infant formulas during storage
2004
Abstract An evaluation is made of the effects of added α -tocopherol ( α -T) or α -tocopherol acetate ( α -TAc) combined with iron lactate or sulfate, storage time (up to 17 months) and storage temperature (22°C and 37°C) on the α -TAc, α -T, γ -tocopherol ( γ -T) and δ -tocopherol ( δ -T) content of infant formulas. Tocopherols were measured by normal phase HPLC and fluorescence detection after direct extraction, which allows the determination of both α -TAc and α -T. The α -TAc content was not affected by temperature or storage time, although the α -, γ - and δ -T contents, which were lower at 37°C than at 22°C, were significantly affected. The type of iron salt added (lactate or sulfate)…
Casein phosphopeptides released by simulated gastrointestinal digestion of infant formulas and their potential role in mineral binding
2006
Abstract Adapted and follow-up milk-based infant formulas were subjected to gastrointestinal digestion simulating physiological conditions. The naturally occurring casein phosphopeptides (CPPs) generated were fractionated by anion exchange high-performance liquid chromatography and sequenced by tandem mass spectrometry. In both infant formula digests, a total of 19 CCPs from bovine casein were identified, of which 7 corresponded to α s1 -casein and 12 to α s2 -casein. Most CPPs had the cluster sequence SpSpSpEE, representing the binding sites for minerals. The distribution of calcium, iron and zinc content in CPP fractions eluted from the anion exchange column was also studied. The results …
Comparison of spectrophotometric and HPLC methods for determining sialic acid in infant formulas
2011
Abstract Two methods for determining sialic acid in infant formulas – spectrophotometry and HPLC with fluorescence detection – have been optimised and validated, the first one allows to determine total sialic acid while the second allows to differentiate the two main forms of sialic acid (N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc)). A common sample preparation procedure (hydrolysis and purification) for both methods has been proposed. The linearity (from 6 to 150 μg of total sialic acid in the assay for spectrophotometry, and from 12.5 to 250 ng and 1 to 5 ng of Neu5Ac and Neu5Gc, respectively, for HPLC) is adequate. The detection and quantification limits (0.29…
INDAGINI PRELIMINARI SULLA PRESENZA DI CRONOBACTER (ENTEROBACTER SAKAZAKII ) NEL LATTE IN POLVERE
2009
Cronobacter ( Enterobacter sakazakii ) is an emerging foodborne pathogen that causes neonatal meningitis , necrotizing enterocolitis and sepsis with case-fatality rates of 20- 50%. Contaminated infant formula has been epidemiologically identifi ed as the source of transmission, although this organism has been isolated from a wide range of foods, food ingredients or food production environments.
Development of a real-time PCR assay for detection and quantification of enterotoxigenic members of Bacillus cereus group in food samples
2009
A highly sensitive real-time PCR (qPCR) procedure, targeting the phosphatidylcholine-specific phospholipase C gene (pc-plc), was developed for specific detection and quantification of strains belonging to Bacillus cereus group. The target region was selected based on the enterotoxigenic profiles of 75 Bacillus strains. The inclusivity and exclusivity of the RTi-PCR assay were assessed with 59 isolates of the B. cereus group, 16 other Bacillus spp., and 4 non-Bacillus strains. The assay was also used to construct calibration curves for different food matrices, and it had a wide quantification range of 6 log units using both serial dilutions of purified DNA and calibrated cell suspensions of …