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RESEARCH PRODUCT
Review: Determination of Vitamin D in Dairy Products by High Performance Liquid Chromatography
Amparo AlegríaSara PeralesReyes BarberáRosaura Farrésubject
Chromatography010405 organic chemistryChemistryGeneral Chemical Engineering010401 analytical chemistryExtraction (chemistry)Vitamina dMass spectrometry01 natural sciencesHigh-performance liquid chromatographyIndustrial and Manufacturing Engineering0104 chemical sciencesInfant formulaBy-productVitamin D and neurologySaponificationFood Sciencedescription
This work reviews the methods used for the determination of vitamin D in some dairy products (milk and infant formulas) by high performance liquid chromatography (HPLC). The low vitamin D contents and the presence of interfering compounds require sample treatment and purification of the extract. The advantages and drawbacks of hot and cold saponification, direct extraction and different types of extract purification are also discussed, taking into account the lack of vitamin D stability by heating, exposure to light and oxidation. With respect to chromatographic determination, the mode (normal or reverse phase), type of column (microcolumn or conventional) and detection system (UV, electrochemical and mass spectrometry) are overviewed, considering the vitamers to be determined.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-12-01 | Food Science and Technology International |