Search results for "Insemination"
showing 10 items of 55 documents
Effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage
2020
The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of …
Parity and number of repeated doe-litter-separation treatments affect differently the reproductive performances of lactating does.
2010
[EN] Using data from three different experiments, the effects of oestrus synchronisation by doe-litter separation (DLS), applied 48 hours befare artificial insemination from day 9 to day 11 post partum, on productive performance of free nursing rabbit does and their litters in relation to parity and number of successive DLS treatments were evaluated. lnitially, 200 New Zealand White does of different parity were homogeneously and definitively distributed in two groups (DLS and Control). On the whole, DLS improved fertility (+23.9%, Ps0.001 ), but reduced the growth of young rabbits from day 9 to weaning (-1.7 g/d, Ps0.01) and their weaning weight (-38 g, Ps0.01) at day 35. DLS also increase…
EFFECT OF A 48H DELAYED INSEMINATION WITH OR WITHOUT A 48H DOE-LITTER SEPARATION ON PERFORMANCE OF NON-RECEPTIVE RABBIT DOES
2010
[EN] The effectiveness of delayed artificial insemination (DAI) of non-receptive (R-) does, with or without oestrus synchronisation by doe-litter separation (MLS), was studied and compared with receptive (R+) does. During 10 months, 105 NZW does, in three homogeneous groups (A, B, C), were artificially inseminated (Al) according to a 42-day reproduction rhythm in two batches. Every 21 days, two Al were effected, the second 48 hours alter the first. The R+ does of ali groups were inseminated al the first Al, while the R- does were treated, in relation to theirgroup, as follows: inseminated at the first Al (A); 48-hour DAI (B); 48-hour MLS by closing the nestbox before Al, when lactating, and…
Effect of controlled nursing with one-day fasting on rabbit doe performance.
2008
This work studied whether the expected better subsequent reproduction for permanent controlled nursing versus free nursing could be further improved by a caloric biostimulation with temporary fasting and re-feeding of does before artificial insemination (AI) and how it influences the development of current litters. A total of 240 females were randomly assigned at first day of lactation to one of three groups each with 80 does in a balanced manner according to the number of kits, litter weight, newborn kit weight after adjusting to 8 rabbits per litter, doe body weight and parity. Rabbits in the control group (C) were fed ad libitum and nursed freely up to weaning at 35 days of age. In the g…
Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination
2021
The aim of the study was to compare the morphometric features of sperm head sizeand shape from the Pietrain line and the Duroc×Pietrain boar crossbred terminal lines, and toevaluate their relationship with reproductive success after artificial insemination of sows producedfrom crossbreeding the York, Landrace and Pietrain breeds. Semen samples were collected from 11sexually mature boars. Only ejaculates with greater than 70% motility rate and <15% of abnormalsperm were used for artificial inseminations (AI) and included in the study. Samples were analyzedusing an ISAS®v1 computer-assisted sperm analysis system for eight morphometric parameters ofhead shape and size (CASA-Morph). Sub-populat…
Relationship between Fertility Traits and Kinematics in Clusters of Boar Ejaculates
2021
The aim was to determine the relationship between kinematic parameters of boar spermatozoa and fertility rates of sow, as well as to assess the effect of sperm clusters on the fertility capacity of the ejaculate. Semen samples were collected from 11 sexually mature boars. Samples were analyzed by an ISAS®v1 CASA-Mot system for eight kinematic parameters. Ejaculate clusters were characterized using multivariate procedures, such as principal factors (PFs) analysis and clustering methods (the k-means model). Four different ejaculate clusters were identified from two kinematic PFs which involved linear trajectory and velocity. There were differences (p 0.05) between dam lines and ejaculate clus…
Effects of a split 48-h doe-litter separation on productivity of free-nursing rabbit does and their litters
2004
To prevent the weight loss of litters, and the distress of both rabbit does and their litters due to doe-litter separation (DLS), without losing its beneficial effects on fertility, the DLS, lasting 48 h before artificial insemination (AI), was split into two periods of 24 h with a short controlled suckling. Initially, 105 does of different parity were divided into three groups. During the post partum, the free-nursing does were treated as follows: regular DLS (R-DLS), the nest-box was closed for 48 h, from days 9 to 11, before AI; split DLS (S-DLS), the nest-box was closed for 24 h and, after suckling, for other 24 h before AI; control, does had free access to the nest-box. Fertility impro…
The sperm of aging male bustards retards their offspring's development.
2015
Understanding whether the sperm of older males has a diminished capacity to produce successful offspring is a key challenge in evolutionary biology. We investigate this issue using 10 years of reproductive data on captive long-lived houbara bustards (Chlamydotis undulata), where the use of artificial insemination techniques means parents can only influence offspring quality via their gametes. Here we show that paternal aging reduces both the likelihood that eggs hatch and the rate at which chicks grow, with older males producing the lightest offspring after the first month. Surprisingly, this cost of paternal aging on offspring development is of a similar scale to that associated with mater…
Sperm competition accentuates selection on ejaculate attributes.
2019
Ejaculate attributes are important factors driving the probability of fertilizing eggs. When females mate with several males, competition between sperm to fertilize eggs should accentuate selection on ejaculate attributes. We tested this hypothesis in the North African houbara bustard ( Chlamydotis undulata undulata ) by comparing the strength of selection acting on two ejaculate attributes when sperm from single males or sperm from different males were used for insemination. In agreement with the prediction, we found that selection on ejaculate attributes was stronger when sperm of different males competed for egg fertilization. These findings provide the first direct comparison of the st…
New In-Depth Analytical Approach of the Porcine Seminal Plasma Proteome Reveals Potential Fertility Biomarkers
2018
A complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis. The SP proteomes of 26 boars showing significan…