Search results for "Integument"

showing 10 items of 754 documents

Hope for Disease-Modifying Treatment of Systemic Sclerosis/Scleroderma

2014

Systemic sclerosis (SSc), or scleroderma, similar to many fibrotic disorders, lacks effective therapies. Current trials focus on anti-inflammatory drugs or targeted approaches aimed at one of the many receptor mechanisms initiating fibrosis. In light of evidence that a myocardin-related transcription factor (MRTF)–and serum response factor (SRF)–regulated gene transcriptional program induced by Rho GTPases is essential for myofibroblast activation, we explored the hypothesis that inhibitors of this pathway may represent novel antifibrotics. MRTF/SRF-regulated genes show spontaneously increased expression in primary dermal fibroblasts from patients with diffuse cutaneous SSc. A novel small-m…

MaleSerum Response Factormedicine.medical_specialtyOncogene Proteins FusionTranscription GeneticNipecotic AcidsDiseaseSclerodermaDrug Discovery and Translational MedicineInternal medicinemedicineAnimalsHumansMyofibroblastsskin and connective tissue diseasesPharmacologyScleroderma Systemicintegumentary systembusiness.industrymedicine.diseaseConnective tissue diseaseDermatologyRheumatologyDNA-Binding Proteinsstomatognathic diseasesMolecular MedicineFemalebusinessJournal of Pharmacology and Experimental Therapeutics
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Wistar rat skin as surrogate for human skin in nortriptyline hydrochloride patch studies

2009

Six different matrices were prepared containing nortriptyline hydrochloride (NTH) with hydroxypropyl-methyl-cellulose as polymer. A mixture of transdermal enhancers was included as part of the vehicle. Diffusion studies were carried out through Wistar rat full thickness skin using Franz cells. They were compared with previously determined human heat separated epidermis in order to test if this animal can be used as model for in vivo assays. A linear correlation was obtained between NTH diffusion coefficients through both skin types (r2=0.996).

MaleSkin AbsorptionPharmaceutical ScienceHuman skinNortriptylineIn Vitro TechniquesPharmacologyAdministration CutaneousDosage formIn vivoFull thickness skinAnimalsMedicineRats WistarSkinTransdermalChromatographyintegumentary systemEpidermis (botany)business.industryRatsNortriptyline HydrochlorideModels AnimalNortriptylinebusinessmedicine.drugInternational Journal of Pharmaceutics
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Global Lung Function Initiative 2012 reference values for spirometry in South Italian children

2017

Rationale Despite the widespread use of the Global Lung Function Initiative (GLI) 2012 reference values, there is still the need of testing their applicability in local areas. Objectives The aims of this study are to evaluate applicability of GLI reference equations in a large population-based sample of normal schoolchildren from Sicily, and to compare GLI and previous prediction equations in terms of spirometry test interpretation. Methods GLI equations were evaluated in 1243 normal schoolchildren, 49% males, aged 7–16 years, height 116–187 cm. Normality assumptions for the GLI z-scores (FEV1, FVC, FEV1/FVC) were tested, and bootstrap confidence intervals for the mean (0 expected) and …

MaleSpirometryPulmonary and Respiratory MedicinePediatricsmedicine.medical_specialtyPulmonary functionAdolescentmedia_common.quotation_subjectVital CapacityStandard scoreWhite PeoplePulmonary function testing03 medical and health sciencesFEV1/FVC ratio0302 clinical medicineReference ValuesForced Expiratory VolumemedicineHumans030212 general & internal medicineChildLungSicilyNormalityLung functionmedia_commonintegumentary systemmedicine.diagnostic_testbusiness.industryZ-scorerespiratory systemrespiratory tract diseasesAirway ObstructionItaly030228 respiratory systemSpirometryPaediatricReference valuesFemaleBootstrap confidence intervalbusinessReference equationcirculatory and respiratory physiologyDemography
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Angiogenesis in Wounds Treated by Microdeformational Wound Therapy.

2011

BACKGROUND:: Mechanical forces play an important role in tissue neovascularization and are a constituent part of modern wound therapies. The mechanisms by which vacuum assisted closure (VAC) modulates wound angiogenesis are still largely unknown. OBJECTIVE:: To investigate how VAC treatment affects wound hypoxia and related profiles of angiogenic factors as well as to identify the anatomical characteristics of the resultant, newly formed vessels. METHODS:: Wound neovascularization was evaluated by morphometric analysis of CD31-stained wound cross-sections as well as by corrosion casting analysis. Wound hypoxia and mRNA expression of HIF-1α and associated angiogenic factors were evaluated by…

MaleVascular Endothelial Growth Factor APathologymedicine.medical_specialtyWound therapyAngiogenesismedicine.medical_treatmentNeovascularization PhysiologicOcclusive DressingsArticleNeovascularizationMiceNegative-pressure wound therapymedicineAnimalsSkinWound Healingintegumentary systemReverse Transcriptase Polymerase Chain Reactionbusiness.industryGranulation tissueBiomechanical PhenomenaMice Inbred C57BLOxygenPlatelet Endothelial Cell Adhesion Molecule-1Occlusive dressingVascular endothelial growth factor Amedicine.anatomical_structureMicrovesselsGranulation TissueCancer researchSurgerymedicine.symptombusinessWound healingNegative-Pressure Wound Therapy
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Effects of massage on the expression of proangiogenic markers in rat skin

2018

Introduction. Massage is a physiotherapeutic treatment, commonly used in both therapy and restoration of normal body functions. The aim of this work was to determine the effects of skin massage on stimulating the expression of angiogenesis-initiating factors, i.e. VEGF-A, FGF-2 (bFGF) and CD34 and on skin regeneration processes. Material and methods. The study was conducted on 48 Buffalo strain rats, randomly divided into two groups. In the first group (M, the massaged group), massage was applied five times a week for 7 weeks. In the second study group (C, the control group), the massage was omitted. Massage consisted of spiral movements at the plantar surface of skin for 5 min on each rear…

MaleVascular Endothelial Growth Factor AskinHistologyCD34FGF-2massageReal-Time Polymerase Chain Reaction01 natural sciencesVEGF-APathology and Forensic MedicineAndrology03 medical and health sciences0302 clinical medicineDownregulation and upregulationGene expressionMedicineAnimalsHumansRegenerationratCell ProliferationMessenger RNAMassageintegumentary systembiology010405 organic chemistrybusiness.industryRegeneration (biology)General MedicineImmunohistochemistry0104 chemical sciencesRatsqPCRGene Expression Regulation030220 oncology & carcinogenesisKi-67biology.proteinImmunohistochemistryKi-67Fibroblast Growth Factor 2CD34businessBiomarkersIHCFolia Histochemica et Cytobiologica
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Avian mite dermatitis: an Italian case indicating the establishment and spread of Ornithonyssus bursa (Acari: Gamasida: Macronyssidae) (Berlese, 1888…

2015

Background Avian mite dermatitis is a skin disease caused in mammals by the incidental bites of blood-sucking mites which customarily parasitize wild and domestic birds. It manifests in the form of pruritic, erythematous, or urticarial papules, with a central sting mark, in skin regions normally covered by clothing. The species mainly implicated in human bite cases are Dermanyssus gallinae, Ornithonyssus sylviarum and, less frequently, Ornithonyssus bursa. The latter is mainly a tropical and subtropical mite and its – presumably transitory – presence has been recorded only once in Europe, in migratory birds. Case report We report a case of avian mite dermatitis in a 70-year-old man, an owne…

MaleVeterinary medicineMite InfestationsDermanyssus gallinaeDermatitisDermatologymedicine.disease_causeparasitic diseasesInfestationMitemedicineSettore MED/35 - Malattie Cutanee E VenereeAnimalsHumansAcariSicilyPoultry DiseasesAgedMitesintegumentary systembiologyOrnithonyssus bursa human infestation Alien speciesEuropebiology.organism_classificationArthropod mouthpartsStingMacronyssidaeOrnithonyssus bursaIntroduced SpeciesChickensInternational journal of dermatology
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GeneticParameters for Milk Somatic Cell Score and Relationships with Production Traits in Primparous Dairy Sheep

2007

A total of 13,066 first-lactation test-day records of 2,277 Valle del Belice ewes from 17 flocks were used to estimate genetic parameters for somatic cell scores (SCS) and milk production traits, using a repeatability test-day animal model. Heritability estimates were low and ranged from 0.09 to 0.14 for milk, fat, and protein yields, and contents. For SCS, the heritability of 0.14 was relatively high. The repeatabilities were moderate and ranged from 0.29 to 0.47 for milk production traits. The repeatability for SCS was 0.36. Flock-test-day explained a large proportion of the variation for milk production traits, but it did not have a big effect on SCS. The genetic correlations of fat and …

MaleVeterinary medicineSomatic cellyieldsselectionBiologyAnimal Breeding and Genomicsheritabilitydairy sheepgenetic parameterAnimal scienceAnimal modelPregnancyfatGenetic variationGeneticsAnimalsLactationFokkerij en Genomicamilk productionlactation curvesSheepModels Geneticintegumentary systemsomatic cell countGenetic VariationewesHeritabilityMilk productionDairyingParityMilkPhenotypenervous systemcountprotein percentagemastitis resistanceTraitWIASAnimal Science and ZoologyFemaleFlockanimal-modelSomatic cell counttissuesFood Science
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Xenon Improves Neurologic Outcome and Reduces Secondary Injury Following Trauma in an In Vivo Model of Traumatic Brain Injury*

2014

Objectives: To determine the neuroprotective efficacy of the inert gas xenon following traumatic brain injury and to determine whether application of xenon has a clinically relevant therapeutic time window. Design: Controlled animal study. Setting: University research laboratory. Subjects: Male C57BL/6N mice (n = 196). Interventions: Seventy-five percent xenon, 50% xenon, or 30% xenon, with 25% oxygen (balance nitrogen) treatment following mechanical brain lesion by controlled cortical impact. Measurements and Main Results: Outcome following trauma was measured using 1) functional neurologic outcome score, 2) histological measurement of contusion volume, and 3) analysis of locomotor functio…

MaleXenonINTRACRANIAL-PRESSURE1110 NursingCritical Care and Intensive Care MedicineGAIT ABNORMALITIESXenonGaitIntracranial pressureintegumentary systemBrainGLYCINE SITEINTRACEREBRAL-HEMORRHAGED-ASPARTATE RECEPTORNeuroprotective AgentsTreatment OutcomeAnesthesiahead traumaneuroprotectionLife Sciences & BiomedicinePOTASSIUM CHANNELSLocomotioncirculatory and respiratory physiologyinorganic chemicalsTraumatic brain injurychemistry.chemical_elementNeuroprotection1117 Public Health and Health ServicesHead traumaCritical Care MedicineIn vivoGeneral & Internal MedicineAdministration InhalationmedicineAnimalscardiovascular diseasesIntracerebral hemorrhageScience & Technologybusiness.industry1103 Clinical Sciencesbrain injurymedicine.diseaseCONTROLLED CORTICAL IMPACTCOMPETITIVE-INHIBITIONEmergency & Critical Care MedicineMice Inbred C57BLDisease Models AnimalCOGNITIVE DEFICITSchemistryBrain InjuriesClosed head injurybusinessCLOSED-HEAD INJURYinert gasesCritical Care Medicine
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Endurance Exercise and Conjugated Linoleic Acid (CLA) Supplementation Up-Regulate CYP17A1 and Stimulate Testosterone Biosynthesis

2013

A new role for fat supplements, in particular conjugated linoleic acid (CLA), has been delineated in steroidogenesis, although the underlying molecular mechanisms have not yet been elucidated. The aims of the present study were to identify the pathway stimulated by CLA supplementation using a cell culture model and to determine whether this same pathway is also stimulated in vivo by CLA supplementation associated with exercise. In vitro, Leydig tumour rat cells (R2C) supplemented with different concentrations of CLA exhibited increasing testosterone biosynthesis accompanied by increasing levels of CYP17A1 mRNA and protein. In vivo, trained mice showed an increase in free plasma testosterone…

Maleendocrine systemmedicine.medical_specialtyLinoleic acidConjugated linoleic acidlcsh:MedicineBiologyMicechemistry.chemical_compoundDownregulation and upregulationEndurance trainingIn vivoCell Line TumorPhysical Conditioning AnimalInternal medicinemedicineAnimalsLinoleic Acids ConjugatedTestosteroneRNA Messengerlcsh:ScienceTestosteroneMultidisciplinaryintegumentary systemSettore BIO/16 - Anatomia UmanaCholesterollcsh:RSteroid 17-alpha-Hydroxylasefood and beveragesRatsUp-RegulationEndocrinologychemistryCell cultureDietary SupplementsPhysical Endurancelcsh:Qlipids (amino acids peptides and proteins)testosterone CLAResearch ArticlePLoS ONE
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The differential influences of melanocortins on nociception in the formalin and tail flick tests

2006

Melanocortins exert multiple physiological effects that include the modulation of immune responses, inflammation processes, and pain transmission. In the present study we investigated the peripheral activity of natural melanocortins - alpha-, beta-, gamma1- and gamma2-melanocyte stimulating hormone (MSH) - and melanocortin receptor subtypes 3 and 4 (MC3/4 receptor) antagonist HS014 in pain (formalin and tail flick) tests after peptide subcutaneous administration in mice. In the formalin test, among all substances tested only alpha-MSH (1 micromol/kg) statistically significantly inhibited the formalin-induced first phase pain response, however, all tested peptides (except gamma1-MSH) at the …

Maleendocrine systemmedicine.medical_specialtyMelanocyte-stimulating hormonemedicine.drug_classClinical BiochemistryAnalgesicNitric OxideToxicologyPeptides CyclicBiochemistryMicegamma-MSHBehavioral NeuroscienceMelanocortin receptorInternal medicinebeta-MSHmedicineAnimalsBiological PsychiatryPain MeasurementMelanocortinsPharmacologyAnalgesicsMice Inbred ICRintegumentary systemChemistryReceptors MelanocortinAntagonistReceptor antagonistMelanocortinsNociceptionEndocrinologyalpha-MSHhormones hormone substitutes and hormone antagonistsTail flick testPharmacology Biochemistry and Behavior
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