Search results for "Internal standard"
showing 4 items of 14 documents
Analyte estimation using the generalized H-point standard additions method and a new methodology for locating linear spectral intervals for unknown i…
1998
An improvement in the way to locate linear spectral intervals for unknown interferents which overlap the analyte signal in UV–visible spectrophotometry is offered as an alternative to the method previously described by Campins-Falco et al. (Anal. Chim. Acta, 302, 323 (1995)). In that report, quotients of the second-derivative spectra of the sample and analyte were used. The new method improves on the old one by using first- instead of second-derivative data, thus eliminating the possible divisions by zero (inflection points for the analyte) obtained in the previous procedure. The linear intervals are now obtained by plotting for each wavelength the differences in first-derivative data betwe…
The generalized H-point standard-additions method to determine analytes present in two different chemical forms in unknown matrix samples. Part I. Ge…
2000
This paper shows how the generalized H-point standard-additions method (GHPSAM) can be used to obtain the total concentration or concentrations of different chemical forms of an analyte when the matrix of the sample is completely unknown. The spectral regions where the interferent behaviour can be considered as linear are found and the analyte concentration free from bias error is estimated. This paper includes the already published features of the GHPSAM and a new modification of this method which allows the simultaneous determination of two chemical forms of an analyte in a sample.
Validation of a set of reference genes to study response to herbicide stress in grasses
2012
Abstract Background Non-target-site based resistance to herbicides is a major threat to the chemical control of agronomically noxious weeds. This adaptive trait is endowed by differences in the expression of a number of genes in plants that are resistant or sensitive to herbicides. Quantification of the expression of such genes requires normalising qPCR data using reference genes with stable expression in the system studied as internal standards. The aim of this study was to validate reference genes in Alopecurus myosuroides, a grass (Poaceae) weed of economic and agronomic importance with no genomic resources. Results The stability of 11 candidate reference genes was assessed in plants res…
A multiresidue analytical method for trace level determination of antibiotics and antiretroviral drugs in wastewater and surface water using SPE-LC-M…
2016
An analytical method for simultaneous determination of seven commonly used antibiotics and three antiretrovirals in surface water and urban wastewater at the ng L−1 level has been developed. The method involves concentration and clean-up by solid phase extraction (SPE) followed by identification and quantification by liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS). The use of matrix-matched calibration curves constructed by spiking surface water was evaluated for quantification and compared with the internal standard method using isotopically labelled compounds. The method gave absolute recoveries of 41–116% with most of the compounds having recoveries >50%. The LOQs ranged fr…